DNA damage in bone marrow cells of mouse males in vivo after exposure to the pheromone: Comet assay

Даев, Е. В.; Петрова, М. В.; Онопа, Л. С.; Shubina, V. A.; Glinin, T. S.

doi:10.1134/s1022795417100027

Cited by 5 publications

(5 citation statements)

References 26 publications

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“…Probably, the detected effect is caused by the non-adaptation of the animals to the chemosignal used at the level of the bone marrow interphase nuclei. It is also possible that the damaged cells will accumulate after 24 h due to their inability to enter mitosis [37,38]. Accordingly, comet assay was used to evaluate DNA damages in apoptotic cells due to its easy implementation and low costs.…”

Section: Olive Tail Momentmentioning

confidence: 99%

Assessment of Genotoxicity of Citrobacter freundii Bacteriocin on Bone Marrow Cells in Albino Mice

Hammad

Obaid

2020

eijs

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Genotoxic effects of crude bacteriocin extracted from Citrobacter freundii were detected on albino mice bone marrow cells in vivo, using micronucleus (MN) and comet assay. The mice were administered intraperitoneally with 37.5, 75, 150 and 300 mg/kg of the extract for 24 hours. C. freundii was isolated from patients suffering from urinary tract infections (UTI). The bacteriocin producing isolates were determined using cup assayand the most efficient bacteriocin producers were selected. Bacteriocin was extracted from the efficient isolates via the induction with Mitomycin-C (2 mg/ml). Bacteriocin activity (320 U/ml) was determined by well diffusion method, while the protein concentration (2900µg/ml) was estimated by Lowery method. The results showed an acute dose-dependent toxic effect of the crude bacteriocin ; The higher doses (150 and 300 mg/kg) caused a significant increase (P≤0.05) in the micronuclei frequency in the bone marrow cells (4.62 and 5.37%, respectively (. Furthermore, DNA damage increased significantly (P≤0.05) and proportionally to higher bacteriocin doses (75, 150 and 300 mg/kg), as demonstrated by increased values of tail length (145.18, 267.73 and 295.08 %,( %DNA in tail (8.05, 13.87 and 14.31 %(, and olive tail moment (13.25, 22.72 and 25.85 % , respectively.

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Section: Olive Tail Momentmentioning

confidence: 99%

Assessment of Genotoxicity of Citrobacter freundii Bacteriocin on Bone Marrow Cells in Albino Mice

Hammad

Obaid

2020

eijs

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“…For the comet assay, the cell suspension was dissolved to the final concentration of ~10 5 cells/mL. The alkaline comet assay was performed in accordance with the standard procedure with small modifications (Daev et al, 2017). The cell suspension (150 μL per specimen) was mixed with an equal volume of 37 °С 1 % solution of low-melting agarose (t m < 42 °C) in microcentrifuge tubes.…”

Section: Comet Assaymentioning

confidence: 99%

Genome response of hippocampal cells to stress in male rats with different excitability of the nervous system

Shcherbinina

Vaido

Khlebaeva

et al. 2022

BioComm

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Changes of genome stability in hippocampal cells of male rats with hereditary high and low thresholds of nervus tibialis response to electric stimuli (HT and LT strains, respectively) were studied in unstressed and stressed animals. HT and LT originated from Wistar strain, males of which were also used as a control. The comet assay was used after prolonged emotional painful stressor action. There were no interstrain differences in the spontaneous percentage of DNA in comet tails (tDNA). However, the prolonged emotional pain stressor induced genome instability differently in animals of different strains. The highest level of DNA damage in hippocampal cells was shown in highly sensitive animals of LT strain. Males of Wistar strain had intermediate levels of tDNA, while HT animals had the lowest stress reactivity.

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“…При этом в сперма-тоцитах нарушается процесс мейотической конъюгации при образовании бивалентов [195], а в клетках кост-ного мозга стрессированных хемосигналами мышей методом ДНК-комет выявлено усиление фрагмента-ции ДНК [199].…”

Section: Genetic Basis Of Ecosystems Evolutionunclassified

“…Вероятно, именно конформационные изменения хроматина приводят к появлению множест-ва щелочнолабильных сайтов и усилению фрагментации ДНК при, например, стрессе у мышей индуцированном феромонами [199]. Последний усиливает также процес-сы активации промутагенов в печени мышей, что свя-зано с изменением активности ферментов системы ци-тохромов Р450 (скорее всего, монооксигеназ CYP1A2 и CYP1B1) [215].…”

Section: возможные механизмы структурной нестабильности генома при стunclassified

Genome and stress-reaction in animals and humans

Dyuzhikova

Алековна²,

Даев

et al. 2018

Ecological genetics

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Current data on the effects of stress at the level of the cell genomes of the central nervous system and peripheral organs in animals are discussed. Regulatory and structural genomic changes in the cells of the central nervous system under stress are considered as a mechanism for regulating the functions of the brain and peripheral organs that form the organism manifestations of stress. Based on the Yu.Ya. Kerkis and M.E. Lobashev point of view, we consider stress as a special physiological state of the nervous system, affecting the work and integrity of the genome in target cells in animals, and thus playing a major role in microevolutionary transformations.

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DNA damage in bone marrow cells of mouse males in vivo after exposure to the pheromone: Comet assay

Cited by 5 publications

References 26 publications

Assessment of Genotoxicity of Citrobacter freundii Bacteriocin on Bone Marrow Cells in Albino Mice

Assessment of Genotoxicity of Citrobacter freundii Bacteriocin on Bone Marrow Cells in Albino Mice

Genome response of hippocampal cells to stress in male rats with different excitability of the nervous system

Genome and stress-reaction in animals and humans

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