DNA damage kinetics (micronucleus and comet assay) in buccal mucosa cells of subjects after metal fixed appliances

Fracasso, Maria Enrica; Doria, Denise; Cerpelloni, Marzia; Scotton, A.; Marchiori, Monique; Faccioni, Fiorenzo

doi:10.1016/j.toxlet.2011.05.245

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“…The assays were conducted in the laboratory of the Section of Pharmacology, University of Verona, Italy ( Faccioni et al 2003 ; Fracasso et al 2009 , 2010 , 2011 ).…”

Section: Methodsmentioning

confidence: 99%

Outdoor Formaldehyde and NO ₂ Exposures and Markers of Genotoxicity in Children Living Near Chipboard Industries

Marcon

Fracasso

Marchetti

et al. 2014

Environ Health Perspect

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Background: Industrial air pollution is a public health hazard. Previous evidence documented increased respiratory symptoms and hospitalizations in children who live near the factories in the largest chipboard manufacturing district in Italy (Viadana).Objectives: We evaluated the association of outdoor exposure to formaldehyde and nitrogen dioxide (NO2) with markers of early genotoxic damage in oral mucosa cells of randomly selected children (6–12 years of age) living in Viadana.Methods: In 2010–2011, DNA strand breaks and nuclear abnormalities were evaluated in exfoliated buccal cells by the comet and micronucleus assays, respectively, and formaldehyde and NO2 were monitored by passive sampling. Annual exposure estimates to pollutants were assigned to children’s houses by spatial interpolation.Results: Of 656 children, 413 (63%) participated. Children living near (< 2 km) the chipboard industries had the highest average exposure to formaldehyde and NO2 (p < 0.001). A 1-SD increase in formaldehyde (0.20 μg/m3) was associated with a 0.13% (95% CI: 0.03, 0.22%) higher comet tail intensity, a 0.007 (95% CI: 0.001, 0.012) higher tail moment, and a 12% relative increase [relative risk (RR) = 1.12; 95% CI: 1.02, 1.23] in nuclear buds. A 1-SD NO2 increase (2.13 μg/m3) was associated with a 0.13% (95% CI: 0.07, 0.19%) increase in binucleated cells and a 16% relative increase (RR = 1.16; 95% CI: 1.06, 1.26) in nuclear buds.Conclusions: Exposure to pollutants was associated with markers of genotoxicity in exfoliated buccal cells of children living in a region with chipboard industries. These findings, combined with previously reported associations between chipboard industrial activities and respiratory outcomes in children, add to concerns about potential adverse effects of industry-related exposures in the Viadana district.Citation: Marcon A, Fracasso ME, Marchetti P, Doria D, Girardi P, Guarda L, Pesce G, Pironi V, Ricci P, de Marco R. 2014. Outdoor formaldehyde and NO2 exposures and markers of genotoxicity in children living near chipboard industries. Environ Health Perspect 122:639–645; http://dx.doi.org/10.1289/ehp.1307259

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“…The assays were conducted in the laboratory of the Section of Pharmacology, University of Verona, Italy ( Faccioni et al 2003 ; Fracasso et al 2009 , 2010 , 2011 ).…”

Section: Methodsmentioning

confidence: 99%

Outdoor Formaldehyde and NO ₂ Exposures and Markers of Genotoxicity in Children Living Near Chipboard Industries

Marcon

Fracasso

Marchetti

et al. 2014

Environ Health Perspect

View full text Add to dashboard Cite

show abstract

DNA damage kinetics (micronucleus and comet assay) in buccal mucosa cells of subjects after metal fixed appliances

Cited by 1 publication

References 0 publications

Outdoor Formaldehyde and NO ₂ Exposures and Markers of Genotoxicity in Children Living Near Chipboard Industries

Outdoor Formaldehyde and NO ₂ Exposures and Markers of Genotoxicity in Children Living Near Chipboard Industries

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DNA damage kinetics (micronucleus and comet assay) in buccal mucosa cells of subjects after metal fixed appliances

Cited by 1 publication

References 0 publications

Outdoor Formaldehyde and NO 2 Exposures and Markers of Genotoxicity in Children Living Near Chipboard Industries

Outdoor Formaldehyde and NO 2 Exposures and Markers of Genotoxicity in Children Living Near Chipboard Industries

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Outdoor Formaldehyde and NO ₂ Exposures and Markers of Genotoxicity in Children Living Near Chipboard Industries

Outdoor Formaldehyde and NO ₂ Exposures and Markers of Genotoxicity in Children Living Near Chipboard Industries