DNA damage levels in electronics workers in Southern China: A micro-whole blood comet assay

Zhao, Zhiqiang; Xing, Xiumei; Liu, Xinxia; Zhou, Ridong; Zhang, Huimin; Liu, Yang; Zhuang, Zhixiong; Su, Xiaolin; Lu, Yao; Jiang, Jun; Yang, Yarui; Dong, Chao; He, Yun

doi:10.1016/j.mrfmmm.2017.07.005

Cited by 10 publications

(4 citation statements)

References 36 publications

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“…An increasing number of human biomonitoring studies with the comet assay have used whole blood, thus avoiding the separation procedure [149][150][151]. In this case only nucleated cells, i.e.…”

Section: Different Blood-derived Preparations and Cell Typesmentioning

confidence: 99%

Application of the comet assay in human biomonitoring: An hCOMET perspective

Azqueta

Ladeira

Giovannelli

et al. 2020

Mutation Research/Reviews in Mutation Research

118

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The comet assay is a well-accepted biomonitoring tool to examine the effect of dietary, lifestyle, environmental and occupational exposure on levels of DNA damage in human cells. With such a wide range of determinants for DNA damage levels, it becomes challenging to deal with confounding and certain factors are interrelated (e.g. poor nutritional intake may correlate with smoking status). This review describes the effect of intrinsic (i.e. sex, age, tobacco smoking, occupational exposure and obesity) and extrinsic (season, environmental exposures, diet, physical activity and alcohol consumption) factors on the level of DNA damage measured by the standard or enzyme-modified comet assay. Although each factor influences at least one comet assay endpoint, the collective evidence does not indicate single factors have a large impact. Thus, controlling for confounding may be necessary in a biomonitoring study, but none of the factors is strong enough to be regarded a priori as a confounder. Controlling for confounding in the comet assay requires a case-by-case approach. Inter-laboratory variation in levels of DNA damage and to some extent also reproducibility in biomonitoring studies are issues that have haunted the users of the comet assay for years. Procedures to collect specimens, and their storage, are not standardized. Likewise, statistical issues related to both sample-size calculation (before sampling of specimens) and statistical analysis of the results vary between studies. This review gives guidance to statistical analysis of the typically complex exposure, co-variate, and effect relationships in human biomonitoring studies.

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Section: Different Blood-derived Preparations and Cell Typesmentioning

confidence: 99%

Application of the comet assay in human biomonitoring: An hCOMET perspective

Azqueta

Ladeira

Giovannelli

et al. 2020

Mutation Research/Reviews in Mutation Research

118

View full text Add to dashboard Cite

show abstract

“…In many cases, subjects were exposed to mixtures of different organic solvents or mixtures of solvents and other toxicants such as heavy metals, PAHs, or pesticides. Around 40% of the studies (n = 20) evaluated workers in factories (plastics, polymers, shoemaking and others) [ 96 , 97 , 363 , 365 , 368 , 372 , 373 , 374 , 375 , 376 , 378 , 379 , 380 , 384 , 385 , 387 , 393 , 394 , 395 , 397 ], a quarter (n = 12; 24.0%) assessed gas station and petrochemical industry workers [ 70 , 369 , 371 , 377 , 381 , 387 , 388 , 389 , 390 , 391 , 392 , 396 ] and fewer studies addressed painters (n = 6; 12.0%) [ 96 , 364 , 366 , 382 , 383 , 386 ], dry cleaners (n = 2; 4.0%) [ 66 , 362 ], biomedical laboratory workers (n = 1; 2.0%) [ 385 ], sewage workers (n = 1; 2.0%) [ 41 ], and employees in biomass fuel burning (n = 1; 2.0%) [ 144 ]. Nine studies (18.0%) evaluated exposure to pollutants in adults [ 49 , 104 , 121 , ...…”

Section: Resultsmentioning

confidence: 99%

The Comet Assay as a Tool in Human Biomonitoring Studies of Environmental and Occupational Exposure to Chemicals—A Systematic Scoping Review

Ladeira,

Møller,

Giovannelli

et al. 2024

Toxics

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Biomonitoring of human populations exposed to chemical substances that can act as potential mutagens or carcinogens, may enable the detection of damage and early disease prevention. In recent years, the comet assay has become an important tool for assessing DNA damage, both in environmental and occupational exposure contexts. To evidence the role of the comet assay in human biomonitoring, we have analysed original research studies of environmental or occupational exposure that used the comet assay in their assessments, following the PRISMA-ScR method (preferred reporting items for systematic reviews and meta-analyses extension for scoping reviews). Groups of chemicals were designated according to a broad classification, and the results obtained from over 300 original studies (n = 123 on air pollutants, n = 14 on anaesthetics, n = 18 on antineoplastic drugs, n = 57 on heavy metals, n = 59 on pesticides, and n = 49 on solvents) showed overall higher values of DNA strand breaks in the exposed subjects in comparison with the unexposed. In summary, our systematic scoping review strengthens the relevance of the use of the comet assay in assessing DNA damage in human biomonitoring studies.

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“…They can cause many pathological effects such as carcinogenesis and reproductive system problems [ 6–8 ]. It is now recognized that genotoxicity caused by exogenous factors is a significant health threat [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Investigation of genotoxic effect of octyl gallate used as an antioxidant food additive in in vitro test systems

2023

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Several antioxidant food additives are added to oils, soups, sauces, chewing gum, potato chips etc. One of them is octyl gallate. The purpose of this study was to evaluate the potential genotoxicity of octyl gallate in human lymphocytes, using in vitro chromosomal abnormalities (CA), sister chromatid exchange (SCE), cytokinesis block micronucleus cytome (CBMN-Cyt), micronucleus-FISH (MN-FISH), and comet tests. Different concentrations (0.50, 0.25, 0.125, 0.063, and 0.031 μg/mL) of octyl gallate were used. A negative (distilled water), a positive (0.20 μg/mL Mitomycin-C), and a solvent control (8.77 μL/mL ethanol) were also applied for each treatment. Octyl gallate did not cause changes in chromosomal abnormalities, micronucleus, nuclear bud (NBUD) and nucleoplasmic bridge (NPB) frequency. Similarly, there was no significant difference in DNA damage (comet assay), percentage of centromere positive and negative cells (MN-FISH test) compared to the solvent control. Moreover, octyl gallate did not affect replication and nuclear division index. On the other hand, it significantly increased the SCE/cell ratio in three highest concentrations compared to solvent control at 24 h treatment. Similarly, at 48 h treatment, the frequency of SCE raised significantly compared to solvent controls at all the concentrations (except 0.031 μg/mL). An important reduce was detected in mitotic index values in highest concentration at 24 h treatment and almost all concentrations (except 0.031 and 0.063 µg / mL) at 48 h treatment. The results obtained suggest that octyl gallate has no an important genotoxicological action on human peripheral lymphocytes at the concentrations applied in this study.

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DNA damage levels in electronics workers in Southern China: A micro-whole blood comet assay

Cited by 10 publications

References 36 publications

Application of the comet assay in human biomonitoring: An hCOMET perspective

Application of the comet assay in human biomonitoring: An hCOMET perspective

The Comet Assay as a Tool in Human Biomonitoring Studies of Environmental and Occupational Exposure to Chemicals—A Systematic Scoping Review

Investigation of genotoxic effect of octyl gallate used as an antioxidant food additive in in vitro test systems

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