DNA damage under simulated extraterrestrial conditions in bacteriophage T7

Fekete, Andrea; Módos, Károly; Hegedüs, M.; Kovács, G.; Rontó, Gy.; Péter, Á.; Lämmer, H.; Panitz, Corinna

doi:10.1016/j.asr.2005.06.076

Cited by 12 publications

(7 citation statements)

References 29 publications

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“…Concerning Uracil thin film as detector, it has been shown that the evaporated polycrystalline substance can function correctly both on the Earth's surface (Bérces et al 1999; Munakata et al 2000) and in simulated space conditions (Fekete et al 2005); however, because of the high vacuum in space, the sample holders containing the evaporated uracil samples were vacuum-tightly closed.…”

Section: Discussionmentioning

confidence: 99%

“…https://doi.org/10.1017/S1473550414000287 (Fekete et al 2005); however, because of the high vacuum in space, the sample holders containing the evaporated uracil samples were vacuum-tightly closed. The dark samples, more exactly, their spectra or second derivatives either for uracil or for phage T7, did not show significant changes indicating that no or very few destructions in the uracil ring or of the phage DNA were induced by cosmic ray particles during the 2 years long flight.…”

Section: Discussionmentioning

confidence: 99%

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The PUR Experiment on the EXPOSE-R facility: biological dosimetry of solar extraterrestrial UV radiation

Bérces

Egyeki

Fekete

et al. 2014

International Journal of Astrobiology

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The aim of our experiment Phage and Uracil Response was to extend the use of bacteriophage T7 and uracil biological dosimeters for measuring the biologically effective ultraviolet (UV) dose in the harsh extraterrestrial radiation conditions. The biological detectors were exposed in vacuum-tightly cases in the European Space Agency (ESA) astrobiological exposure facility attached to the external platform of Zvezda (EXPOSE-R). EXPOSE-R took off to the International Space Station (ISS) in November 2008 and was installed on the External platform of the Russian module Zvezda of the ISS in March 2009. Our goal was to determine the dose-effect relation for the formation of photoproducts (i.e. damage to phage DNA and uracil, respectively). The extraterrestrial solar UV radiation ranges over the whole spectrum from vacuum-UV (λ < 200 nm) to UVA (315 nm < λ < 400 nm), which causes photolesions (photoproducts) in the nucleic acids/their components either by photoionization or excitation. However, these wavelengths cause not only photolesions but in a wavelength-dependent efficiency the reversion of some photolesions, too. Our biological detectors measured in situ conditions the resultant of both reactions induced by the extraterrestrial UV radiation. From this aspect the role of the photoreversion in the extension of the biological UV dosimetry are discussed.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The PUR Experiment on the EXPOSE-R facility: biological dosimetry of solar extraterrestrial UV radiation

Bérces

Egyeki

Fekete

et al. 2014

International Journal of Astrobiology

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“…Both the phage and DNA films are attached so strongly to the quartz plates, that they remained totally adsorbed during the vacuum treatment. The samples contracted and supposedly the distance between helices decreased, but the thin layers still remained homogeneously covered [20].…”

Section: Effect Of Vacuummentioning

confidence: 97%

Response of bacteriophage T7 biological dosimeter to dehydration and extraterrestrial solar UV radiation

Hegedüs¹,

Fekete²,

Módos³

et al. 2007

Acta Astronautica

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“…The effects caused by the selected space parameters in the bacteriophage T7 and uracil thin film respectively, have been investigated with several methods: counting the survivor phage particles on infected Escherichia coli B host cells, determination of the UV absorption spectrum and the quantitative characteristics of the spectra [26], quantitative PCR (QPCR), enzymatic digestion, electrophoretic pattern [27,28], in addition, thin layer chromatography (TCL), mass spectroscopy of the photoproducts.…”

Section: Methodsmentioning

confidence: 99%

Biological ultraviolet Dosimetry in Low Earth’s Orbit

Egyeki¹

2013

Astrobiol Outreach

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The biological dosimetry of the solar UV radiation functioned correctly on the Earth's surface. The aim of the present studies was to extend the dosimetry to the extraterrestrial solar radiation in LEO. Similar to the Earth's surface bacteriophage T7 and polycrystalline uracil thin layers were used as detectors and exposed to the simulated and to the real space environmental parameters aiming to perform the in situ biological UV dosimetry in the space, more exactly on the external pallet of the ISS. The UV detectors have been used in specific cases in thin layer form. In contrast to the Earth's surface the extraterrestrial solar UV radiations contains wavelength components (λ ~ 190-200 nm), which cause photolesions (photoproducts) in the nucleic acids/their components similar to the UV-B photons. However, these wavelengths cause not only photolesions but with a wavelength-dependent efficiency the reversion of some photolesion, too. Our biological detectors measured either in simulation or in situ conditions the resultant of both reactions induced by the extraterrestrial UV radiation. From this aspect the role of the photoreversion in the extension of the biological UV dosimetry and in the survival of the living systems in the space are discussed.

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DNA damage under simulated extraterrestrial conditions in bacteriophage T7

Cited by 12 publications

References 29 publications

The PUR Experiment on the EXPOSE-R facility: biological dosimetry of solar extraterrestrial UV radiation

The PUR Experiment on the EXPOSE-R facility: biological dosimetry of solar extraterrestrial UV radiation

Response of bacteriophage T7 biological dosimeter to dehydration and extraterrestrial solar UV radiation

Biological ultraviolet Dosimetry in Low Earth’s Orbit

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