DNA delivery with hyperbranched polylysine: A comparative study with linear and dendritic polylysine

Kadlecova, Zuzana; Rajendra, Yashas; Matasci, Mattia; Baldi, Lucia; Hacker, David L.; Wurm, Florian Μ.; Klok, Harm‐Anton

doi:10.1016/j.jconrel.2013.01.019

Cited by 116 publications

(114 citation statements)

References 49 publications

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“…Hyperbranched PLL is an attractive synthetic vector that has been recently synthesized, and produced at a large scale. In addition, it is completely biodegradable, which can prevent toxicity [49]. Hyperbranched PLL was shown to have in vitro transfection abilities close to PEI [49].…”

Section: The Use Of Poly-l-lysine In Gene Deliverymentioning

confidence: 99%

“…In addition, it is completely biodegradable, which can prevent toxicity [49]. Hyperbranched PLL was shown to have in vitro transfection abilities close to PEI [49]. Amino acids such as arginine or lysine, localized in the hydrophilic head group have an impact on the effectiveness of amino acid-based cationic complexes [50].…”

Section: The Use Of Poly-l-lysine In Gene Deliverymentioning

confidence: 99%

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Synthetic vectors for gene delivery: An overview of their evolution depending on routes of administration.

Belmadi

Midoux

Loyer

et al. 2015

Biotechnology Journal

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Nucleic acid delivery constitutes an emerging therapeutic strategy to cure various human pathologies. This therapy consists of introducing genetic material into the whole body or isolated cells to correct a cellular abnormality or disfunction. As with any drug, the main objective of nucleic acid delivery is to establish optimal balance between efficacy and tolerance. The methods of administration and the vectors used are selected depending on whether the goal of treatment is the production of an active protein; the replacement of a missing or inactive gene; or the combat of acquired diseases, such as cancer or AIDS. In that sense, synthetic vectors represent a valuable solution because they are well characterized, their structure can be fine tuned, and their potential toxicity can be reduced, since toxicity depends on the composition of the formulations. Here we review various synthetic vectors for gene delivery and address the question of their biodistribution as a function of the route of administration. We highlight the modifications to vectors structure and formulations necessary to overcome the major hurdles limiting the effectiveness of nucleic acid therapies.

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Section: The Use Of Poly-l-lysine In Gene Deliverymentioning

confidence: 99%

Section: The Use Of Poly-l-lysine In Gene Deliverymentioning

confidence: 99%

Synthetic vectors for gene delivery: An overview of their evolution depending on routes of administration.

Belmadi

Midoux

Loyer

et al. 2015

Biotechnology Journal

View full text Add to dashboard Cite

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“…However, in vivo use of such complexes may be restricted by toxic effects caused by polymer-induced aggregation of erythrocytes and complex formation with anionic blood proteins. The most frequently used polymers include polylysine and polyethyleneimine (PEI) which are both available in linear and branched versions (Kadlecova et al 2013). Highly branched polymers provide increased flexibility and stable complex formation but are thought also to display higher cytotoxic effects due to their increased affinity for the cell membrane leading to damage of the membrane (Fischer et al 2003;Kadlecova et al 2012).…”

Section: Delivery Of Plasmid Dna Wrapped In Synthetic Carrier Materiamentioning

confidence: 99%

Nonviral Gene Therapy—The Challenge of Mobilizing DNA

Mikkelsen

2015

Somatic Genome Manipulation

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“…The resultant RGDK/ R interact with NRP-1, enhancing the tumor permeability and the tumor accumulation of coadministrated drugs such as nab-paclitaxel (ABX), doxorubicin (DOX), DOX liposomes, and trastuzumab. [16] Polypeptides have found wide uses in biomedical application such as drug/gene delivery, [22][23][24][25][26] tissue engineering, [27,28] diagnostics, [29] and biosensors. [30] Polypeptides can be synthesized by the ring-opening polymerization (ROP) of N-carboxy anhydrides (NCA) of a-amino acids, which has become the most common technique used for large scale preparation of polypeptides with high molecular weight.…”

Section: Introductionmentioning

confidence: 99%

Poly(ornithine‐co‐arginine‐co‐glycine‐co‐aspartic Acid): Preparation via NCA Polymerization and its Potential as a Polymeric Tumor‐Penetrating Agent

Tang

Zhang

et al. 2015

Macromolecular Bioscience

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A novel random copolypeptide of ornithine, arginine, glycine, and aspartic acid [Poly(ornithine-co-arginine-co-glycine-co-aspartic acid), Poly(O,R,G,D)] has been prepared through ring-opening polymerization of N-δ-carbobenzoxy-l-ornithine N-carboxyanhydride [Orn(Cbz)-NCA)], l-glycine N-carboxyanhydride (Gly-NCA) and β-benzyl l-aspartate N-carboxyanhydride [Asp(Bn)-NCA], following by subsequent deprotection and guanidization. The structure of Poly(O,R,G,D) was confirmed by nuclear magnetic resonance (NMR) spectroscopy and gel permeation chromatography (GPC). Low cytotoxicity of Poly(O,R,G,D) was confirmed from MTT assay. The Poly(O,R,G,D) contain some internal sequences of RXXR (X = O, R, G, or D) that could be proteolytically cleaved to expose the cryptic CendR element and bind to Neuropilin-1. This would lead to vascular and tissue permeabilization. Therefore trypsin-cleaved Poly(O,R,G,D) increase the vascular leakage of Evans blue from dermal microvessels at the injection site in vivo skin permeability assay. The intratumoral injection of the Poly(O,R,G,D) significantly enhanced the concentration of cisplatin-loaded nanoparticles in MCF-7 solid tumors. These results show that Poly(O,R,G,D) could increase the vascular leakage and tissue penetration of nanoparticles in a solid tumor and can be used as a potential polymeric tumor-penetrating agent.

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DNA delivery with hyperbranched polylysine: A comparative study with linear and dendritic polylysine

Cited by 116 publications

References 49 publications

Synthetic vectors for gene delivery: An overview of their evolution depending on routes of administration.

Synthetic vectors for gene delivery: An overview of their evolution depending on routes of administration.

Nonviral Gene Therapy—The Challenge of Mobilizing DNA

Poly(ornithine‐co‐arginine‐co‐glycine‐co‐aspartic Acid): Preparation via NCA Polymerization and its Potential as a Polymeric Tumor‐Penetrating Agent

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