DNA Double-strand Break Induction in Yeast by X-rays and α-particles Measured by Pulsed-field Gel Electrophoresis

Löbrich, Markus; Ikpeme, S.; Haub, P.; Weber, Klaus; Kiefer, Jürgen

doi:10.1080/09553009314551751

Cited by 26 publications

(15 citation statements)

References 35 publications

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“…Experimental details of both methods have been published (Akpa et al 1992;L6brich et al 1993). While the first one is a well-known standard procedure, the second is rather new and deserves a few words of explanation.…”

Section: Determination Of Induced Double-strand Breaksmentioning

confidence: 99%

“…For particulate radiation, the yield of DSB s per unit dose has been found to increase with LET up to about 150 keV/gm in yeast cells (Frankenberg et al 1981;Akpa et al 1992;L6brich et al 1993) as well as in earlier studies with mammalian cells (Kampf 1982;Kampf and Eichhorn 1983). Such a LET-RBE (relative biological effectiveness) relationship is consistent with computational Monte-Carlo simulations Chatterjee and Holley 1991).…”

Section: Introductionmentioning

confidence: 95%

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Heavy ion-induced DNA double-strand breaks with yeast as a model system

Ikpeme

Löbrich

Akpa

et al. 1995

Radiat Environ Biophys

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Cells of diploid yeast, Saccharomyces cerevisiae, were exposed to a variety of energetic heavy ions (provided by the UNILAC facility at the Gesellschaft für Schwerionenforschung, GSI), 241Am alpha-particles and 80-keV x-rays after which they were assessed for DNA double-strand breaks (DSB) using either the neutral sedimentation or the pulsed-field gel electrophoresis (PFGE) technique. Both yielded comparable results. The DSB production cross-sections are compared with inactivation studies performed for the same cells under identical conditions. The measurements show that with lighter ions DSB induction cross-sections increase with linear energy transfer (LET), but the situation is less clear with the heavier ions. A close parallelism was found between DSB induction and cell inactivation in these yeast cells.

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Section: Determination Of Induced Double-strand Breaksmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

Heavy ion-induced DNA double-strand breaks with yeast as a model system

Ikpeme

Löbrich

Akpa

et al. 1995

Radiat Environ Biophys

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“…The initial yield of DSBs per unit dose has been found to depend on the quality of the radiation. For a particles, an increase in the yield of DSBs with linear energy transfer (LET) up to about 150 keV/pm has been found using some experimental systems (4)(5)(6), including mammalian cells in early studies (7,8). Such a behavior is also predicted by Monte Carlo simulations in computational studies (9,10).…”

Section: Introductionmentioning

confidence: 97%

DNA Double-Strand Breaks Induced by High-Energy Neon and Iron Ions in Human Fibroblasts. I. Pulsed-Field Gel Electrophoresis Method

Cooper¹,

Rydberg²,

Löbrich³

1994

Radiation Research

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The relative effectiveness of high-energy neon and iron ions for the production of DNA double-strand breaks was measured in one transformed and one nontransformed human fibroblast cell line using pulsed-field gel electrophoresis. The DNA released from the gel plug (fraction of activity released: FAR) as well as the size distribution of the DNA entering the gel were used to compare the effects of the heavy-ion exposure with X-ray exposure. Both methods gave similar results, indicating similar distributions of breaks over megabase-pair distances for the heavy ions and the X rays. The relative biological effectiveness (RBE) compared to 225 kVp X rays of initially induced DNA double-strand breaks was found to be 0.85 for 425 MeV/u neon ions (LET 32 keV/microns) and 0.42-0.55 for 250-600 MeV/u iron ions (LET 190-350 keV/microns). Postirradiation incubation showed less efficient repair of breaks induced by the neon ions and the 600 MeV/u iron ions compared to X rays. Survival experiments demonstrated RBE values larger than one for cell killing by the heavy ions in parallel experiments (neon: RBE = 1.2, iron: RBE = 2.3-3.0, based on D10 values). It is concluded that either the initial yield of DNA double-strand breaks induced by the high-energy particles is lower than the yield for X rays, or the breaks induced by heavy ions are present in clusters that cannot be resolved with the technique used. These results are confirmed in the accompanying paper (M. Löbrich, B. Rydberg and P. Cooper, Radiat. Res. 139, 142-151, 1994).

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“…Switching time was ramped from 30 s to 100 s over a 24 h running time. After electrophoresis, the gels were stained and destained following a procedure described by Lobrich et al (1993). The gel image was photographed under UV light with a polaroid camera.…”

Section: Genetic Assaysmentioning

confidence: 99%

Genetic and physiological alterations occurring in a yeast population continuously propagated at increasing temperatures with cell recycling

Souza

Thomaz

Cides

et al. 2007

World J Microbiol Biotechnol

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This work investigated the effects of increasing temperature from 30°C to 47°C on the physiological and genetic characteristics of Saccharomyces cerevisiae strain 63M after continuous fermentation with cell recycling in a system of five reactors in series. Steady state was attained at 30°C, and then the temperature of the system was raised so it ranged from 35°C in the last reactor to 43°C in the first reactor or feeding reactor with a 2°C difference between reactors. After 15 days at steady state, the temperature was raised from 37°C to 45°C for 25 days at steady state, then from 39°C to 47°C for 20 days at steady state. Starter strain 63M was a hybrid strain constructed to have a MAT a/α, LYS/lys, URA/ura genotype. This hybrid yeast showed vigorous growth on plates at 40°C, weak growth at 41°C, positive assimilation of melibiose, positive fermentation of galactose, raffinose and sucrose. Of 156 isolates obtained from this system at the end of the fermentation process, only 17.3% showed the same characteristics as starter strain 63M. Alterations in mating type reaction and in utilization of raffinose, melibiose, and sucrose were identified. Only 1.9% of the isolates lost the ability to grow at 40°C. Isolates showing requirements for lysine and uracil were also obtained. In addition, cell survival was observed at 39-47°C, but no isolates showing growth above 41°C were obtained.

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DNA Double-strand Break Induction in Yeast by X-rays and α-particles Measured by Pulsed-field Gel Electrophoresis

Cited by 26 publications

References 35 publications

Heavy ion-induced DNA double-strand breaks with yeast as a model system

Heavy ion-induced DNA double-strand breaks with yeast as a model system

DNA Double-Strand Breaks Induced by High-Energy Neon and Iron Ions in Human Fibroblasts. I. Pulsed-Field Gel Electrophoresis Method

Genetic and physiological alterations occurring in a yeast population continuously propagated at increasing temperatures with cell recycling

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