DNA double strand breaks and Hsp70 expression in proton irradiated living cells

Fiedler, Anja; Reinert, Tilo; Tanner, Judith; Butz, Tilman

doi:10.1016/j.nimb.2007.02.020

Cited by 7 publications

(4 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among these are: LIPSION at the University of Leipzig (Germany), which has a “hit accuracy” better than 0.5 µm [12]; the PTB (Physikalisch-Technische Bundesanstalt) microbeam in Braunscweig (Germany), which has a beam diameter of ~2 µm (fwhm) [13]; and the particle irradiation system SPICE (single particle irradiation to cell) at the National Institute of Radiological Sciences (NIRS) in Chiba (Japan) that can produce a beam spot approximately 10 µm in diameter [14]. …”

Section: Discussionmentioning

confidence: 99%

The Columbia University sub-micron charged particle beam

Randers-Pehrson

Johnson

Marino

et al. 2009

Nuclear Instruments and Methods in Physics Research Section A:

View full text Add to dashboard Cite

A lens system consisting of two electrostatic quadrupole triplets has been designed and constructed at the Radiological Research Accelerator Facility (RARAF) of Columbia University. The lens system has been used to focus 6-MeV 4He ions to a beam spot in air with a diameter of 0.8 µm. The quadrupole electrodes can withstand voltages high enough to focus 4He ions up to 10 MeV and protons up to 5 MeV. The quadrupole triplet design is novel in that alignment is made through precise construction and the relative strengths of the quadrupoles are accomplished by the lengths of the elements, so that the magnitudes of the voltages required for focusing are nearly identical. The insulating sections between electrodes have had ion implantation to improve the voltage stability of the lens. The lens design employs Russian symmetry for the quadrupole elements.

show abstract

Section: Discussionmentioning

confidence: 99%

The Columbia University sub-micron charged particle beam

Randers-Pehrson

Johnson

Marino

et al. 2009

Nuclear Instruments and Methods in Physics Research Section A:

View full text Add to dashboard Cite

show abstract

“…Focused spots of 1 μm or less on the samples are however achievable with a very low fraction of particles reaching the targets being scattered. Successful charged particle microbeams based on focus systems have been developed and are routinely used in Germany at the PTB [34], the GSI [35], the University of Munich [31] and Leipzig,[36], in Japan at the NIRS [37] and at Columbia University (USA) [32]. …”

Section: Current Radiation Microbeamsmentioning

confidence: 99%

Radiation microbeams as spatial and temporal probes of subcellular and tissue response

Schettino

Rashid

Prise

2010

Mutation Research/Reviews in Mutation Research

View full text Add to dashboard Cite

Understanding the effects of ionising radiations are key to determining their optimal use in therapy and assessing risks from exposure. The development of microbeams where radiations can be delivered in a highly temporal and spatially constrained manner has been a major advance. Several different types of radiation microbeams have been developed using X-rays, charged particles and electrons. For charged particles, beams can be targeted with sub-micron accuracy into biological samples and the lowest possible dose of a single particle track can be delivered with high reproducibility. Microbeams have provided powerful tools for understanding the kinetics of DNA damage and formation under conditions of physiological relevance and have significant advantages over other approaches for producing localised DNA damage, such as variable wavelength laser beam approaches. Recent studies have extended their use to probing for radiosensitive sites outside the cell nucleus, and testing for mechanisms underpinning bystander responses where irradiated and non-irradiated cells communicate with each other. Ongoing developments include the ability to locally target regions of 3-D tissue models and ultimately to target localised regions in vivo. With future advances in radiation delivery and imaging microbeams will continue to be applied in a range of biological studies.

show abstract

“…Although no online cell recognition is done at this time, the LIPSION system has been irradiating cells, with micrometer scale patterns of 2.35-MeV protons and 2-MeV helium nuclei, since 2003 (e.g., [44]). Particle counting is achieved by placing a movable p-i-n diode after the cells.…”

Section: E Lipsion (Leipzig Germany)mentioning

confidence: 99%

Single-Particle/Single-Cell Ion Microbeams as Probes of Biological Mechanisms

Bigelow

Brenner

Garty

et al. 2008

IEEE Trans. Plasma Sci.

View full text Add to dashboard Cite

Abstract-An ion microbeam is a very narrow beam of charged particles, typically protons, alpha particles, or heavier, of micrometer/submicrometer size, corresponding to cellular/ subcellular dimensions. Together with integrated techniques for locating live cellular or subcellular targets, they allow rapid sequential irradiation of these targets. This review covers both the technology involved in modern single-cell microbeams, as well as some current applications. The recent explosion of interest in microbeams was initially driven by interest in the domestic radon problem, in which target cells are exposed either to zero or one alpha particle. Microbeams allow cells to be individually irradiated with exact numbers of particles. As microbeams were built, refined, and used, the biological questions that were addressed with them have considerably broadened, to encompass many aspects of damage signal transduction. Two areas in particular have attracted much interest: One is the use of microbeams to address the sensitivity of subcellular targets, such as the cytoplasm or mitochondria. The other reflects the ability of the microbeam to irradiate some cells, but not others, allowing a direct investigation of the so-called bystander effect, where signals from irradiated cells can apparently cause biological responses in neighboring unirradiated cells.

show abstract

DNA double strand breaks and Hsp70 expression in proton irradiated living cells

Cited by 7 publications

References 20 publications

The Columbia University sub-micron charged particle beam

The Columbia University sub-micron charged particle beam

Radiation microbeams as spatial and temporal probes of subcellular and tissue response

Single-Particle/Single-Cell Ion Microbeams as Probes of Biological Mechanisms

Contact Info

Product

Resources

About