DNA electrotransfer into the skin using a combination of one high- and one low-voltage pulse

Local and systemic antitumor effect of intratumoral and peritumoral IL-12 electrogene therapy on murine sarcoma

“…22 Comparison of therapeutic efficiency of intratumoral versus intradermal EGT was carried out based on the fact that intradermal gene electrotransfer can result in either local or systemic transgene expression. [39][40][41] Successful intradermal IL-12 gene electrotransfer has already been achieved, resulting in as high as a 10-fold increase of systemic concentrations of IFNγ, compared to control groups, 42 whereas data on possible systemic production of IL-12 after intratumoral delivery is ambiguous. Based on these facts we assumed that with intradermal EGT positioned into the peritumoral region, we will combine highly efficient production of IL-12 both at the systemic and local intratumoral level.…”

Control by pulse parameters of DNA electrotransfer into solid tumors in mice

“…[10][11][12][13][14][15][16] A new protocol with high transfection efficacy in muscle and skin has recently been described, consisting of a combination of one high-field (HV, B1000 V cm À1 ) and one (or several) low-field (LV, B100 V cm À1 ) pulse. [17][18][19][20][21][22][23] For simplicity, we called all along the paper 'field' what indeed is the voltage to electrode width ratio, that is, an experimentally controlled parameter. In these studies, the authors suggested two functions of these electric pulses.…”

Optimization of cutaneous electrically mediated plasmid DNA delivery using novel electrode

“…For electroporation gene delivery to skin, plasmid DNA may be injected intradermally [1][2][3][4][5][6][7][8][9][10] or biolistically. 11 Pulses are applied to the skin surface or through penetrating (needle) electrodes.…”

“…Caliper, plate, tweezer or clip type electrodes grip a fold of skin. 1,[4][5][6]9,10 The meander 7,11 and the N1/ N2 electrode 8 are simply pressed against the injected region. The applied pressure may vary.…”