DNA hybridization detection in a microfluidic channel using two fluorescently labelled nucleic acid probes

Chen, Lingxin; Lee, Sangyeop; Lee, Moonkwon; Lim, Chaesung; Choo, Jaebum; Park, Joong Yull; Lee, Sanghoon; Joo, Sang Woo; Lee, Kyeong Hee; Choi, Young Wook

doi:10.1016/j.bios.2008.02.013

Cited by 56 publications

(24 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All of these are not favourable for the development of miniaturized, low-cost or disposable and rapid diagnostic devices. With the rapid growth of the microfluidic technology, microarrays and microfluidic biochips have been extensively employed as promising tools for DNA analysis over the past decade (Berti et al 2009;Cao et al 2010;Chen et al 2008;Heule and Manz 2004;Javanmard and Davis 2011;Mai et al 2007;Mulvaney et al 2007;Jin et al 2009;Kwakye and Baeumner 2003;Li and He 2009;Senapati et al 2009). The advantages of applying microfluidics platform in DNA hybridization have been demonstrated in many representative works (Fan et al 1999;Erickson et al 2004;Ali et al 2003;Sun and Kwok 2006;Ferguson et al 2009).…”

Section: Introductionmentioning

confidence: 99%

“…By using the microfluidic technology, the complete DNA hybridization assay-including sampling, hybridization reaction, incubation and washing-can be conducted directly on a microfluidic platform in a liquid flow format (i.e., the probe-target reaction (hybridization) occurs in the flowing liquid). Kim et al (2007) and Chen et al (2008) developed a rapid DNA assay in a microfluidic chip of alligator teeth-shaped microchannel network, as shown in Fig. 1.…”

Section: Introductionmentioning

confidence: 99%

“…The dimensions of the microchannels were illustrated in the picture (unit: mm). The four rectangular areas were the fluorescence detection sites (Chen et al 2008) Fig. 2 Schematic and a picture of the electrochemical DNA sensor.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Microfluidic DNA hybridization assays

Weng

Jiang

2011

Microfluid Nanofluid

View full text Add to dashboard Cite

DNA hybridization is one of the most powerful techniques applied in diagnostic assays. Microfluidics provides a promising means to analyse small sample volumes, reduce reagent consumption and cost, shorten processing time as well as develop fast, sensitive and portable diagnostic tools. By coupling with the microfluidic technology, DNA hybridization assay can achieve high sensitivity, enhance hybridization kinetics and decrease the non-specific target-probe binding. The microfluidic-based DNA hybridization technology has a great potential for developing low-cost, rapid, automatic and point-of-care diagnostic devices. In this article, we provide an overview and summarize the recent advances on the merging of microfluidics to DNA hybridization assays. The advantages and disadvantages of various methods are discussed. Potential improvements required for these technologies are proposed as well.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Microfluidic DNA hybridization assays

Weng

Jiang

2011

Microfluid Nanofluid

View full text Add to dashboard Cite

show abstract

“…Upon reasonable design, such conformational change may be applied for varying the local environment of fluorophores or nanomaterials, and therefore altering the emission properties of fluorescent systems. Compared to colorimetry, fluorescence approaches show more promising applications in measuring bio-interactions in aquatic environment because of the flexible ways in quantitative analysis with high sensitivity and wide response range (Chen et al, 2008b;Kim et al, 2007;Jung et al, 2007). Traditional organic dyes associate with the problem of narrow absorption, broad emission, photobleaching and so on, unable to meet the expectations.…”

Section: Fluorometric Assaymentioning

confidence: 99%

Nanomaterial-assisted aptamers for optical sensing

Wang

Chen

et al. 2010

Biosensors and Bioelectronics

Self Cite

242

View full text Add to dashboard Cite

a b s t r a c tAptamers are single-strand DNA or RNA selected in vitro that bind specifically with a broad range of targets from metal ions, organic molecules, to proteins, cells and microorganisms. As an emerging class of recognition elements, aptamers offer remarkable convenience in the design and modification of their structures, which has motivated them to generate a great variety of aptamer sensors (aptasensors) that exhibit high sensitivity as well as specificity. On the other hand, the development of nanoscience and nanotechnology has generated nanomaterials with novel properties compared with their counterparts in macroscale. By integrating their strengths of both fields, recently, versatile aptamers coupling with novel nanomaterials for designing nanomaterial-assisted aptasensors (NAAs) make the combinations universal strategies for sensitive optical sensing. NAAs have been considered as an excellent sensing platform and found wide applications in analytical community. In this review, we summarize recent advances in the development of various optical NAAs, employing various detection techniques including colorimetry, fluorometry, surface-enhanced Raman scattering (SERS), magnetic resonance imaging (MRI) and surface plasmon resonance (SPR).

show abstract

“…DNA hybridization assays 59 provide more sensitive, specific, and rapid detection of target nucleic acids when compared to conventional antibody-based assays. Many microfluidic DNA-based probes coupled with techniques such as fluorescence resonance energy transfer (FRET), 60 conductance impedance, 61 and surface plasmon resonance imaging (SPRi) 62 have been reported. Peptide nucleic acid (PNA) is a DNA mimic and has a peptide backbone instead of a sugar phosphate backbone.…”

Section: Antibodies Antibody Alternatives and Dnabased Assaysmentioning

confidence: 99%

Development and Applications of Portable Biosensors

2015

View full text Add to dashboard Cite

The significance of microfluidics-based and microelectromechanical systems-based biosensors has been widely acknowledged, and many reviews have explored their potential applications in clinical diagnostics, personalized medicine, global health, drug discovery, food safety, and forensics. Because health care costs are increasing, there is an increasing need to remotely monitor the health condition of patients by point-of-care-testing. The demand for biosensors for detection of biological warfare agents has increased, and research is focused on ways of producing small portable devices that would allow fast, accurate, and on-site detection. In the past decade, the demand for rapid and accurate on-site detection of plant disease diagnosis has increased due to emerging pathogens with resistance to pesticides, increased human mobility, and regulations limiting the application of toxic chemicals to prevent spread of diseases. The portability of biosensors for onsite diagnosis is limited due to various issues, including sample preparation techniques, fluid-handling techniques, the limited lifetime of biological reagents, device packaging, integrating electronics for data collection/analysis, and the requirement of external accessories and power. Many microfluidic, electronic, and biological design strategies, such as handling liquids in biosensors without pumps/valves, the application of droplet-based microfluidics, paper-based microfluidic devices, and wireless networking capabilities for data transmission, are being explored.

show abstract

DNA hybridization detection in a microfluidic channel using two fluorescently labelled nucleic acid probes

Cited by 56 publications

References 31 publications

Microfluidic DNA hybridization assays

Microfluidic DNA hybridization assays

Nanomaterial-assisted aptamers for optical sensing

Development and Applications of Portable Biosensors

Contact Info

Product

Resources

About