DNA hybridization sensor based on AlGaN/GaN HEMT

Alur, Siddharth; Thapa, Resham; Gnaprakasa, Tony; Wang, Y.; Sharma, Yogesh; Javalosa, Edritz; Smith, Elizabeth; Ahyi, Claude; Simonian, Aleksandr; Bozack, Michael J.; Williams, John R.; Park, Minseo

doi:10.1002/pssc.201001164

Cited by 7 publications

(7 citation statements)

References 8 publications

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“…Salmonella are typically found in the intestinal tracts of humans and other animals, and infection occurs through consumption of food contaminated with fecal matter or through unsafe food preparation methods. 137 Pathogenic Salmonella has been detected using antibodies, 124,[138][139][140][141] antimicrobial peptides, 127,128 bacteriophages, 85,142 and DNA probes 81,[143][144][145] coupled with optical, 124,127,128,138,145 electrochemical, 81,141,144 and mass sensitive 85,139,142 transduction techniques. Nyquist-Battie et al examined the effects of low-shear modeled microgravity (LSMMG) on antibodies and the growth of Salmonella, for monitoring food and water during spaceflight.…”

Section: Salmonellamentioning

confidence: 99%

Biosensor technology: recent advances in threat agent detection and medicine

et al. 2013

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Biosensors are of great significance because of their capability to resolve a potentially large number of analytical problems and challenges in very diverse areas such as defense, homeland security, agriculture and food safety, environmental monitoring, medicine, pharmacology, industry, etc. The expanding role of biosensing in society and a real-world environment has led to an exponential growth of the R&D efforts around the world. The world market for biosensor devices, according to Global Industry Analysts, Inc., is expected to reach $12 billion by 2015. Such expedient growth is driven by several factors including medical and health problems, such as a growing population with a high risk of diabetes and obesity, and the rising incidence of chronic diseases such as heart disease, stroke, cancer, chronic respiratory diseases, tuberculosis, etc.; significant problems with environmental monitoring; and of course serious challenges in security and military applications and agriculture/food safety. A review paper in the biosensor technology area may be structured based on (i) the principles of detection, such as the type of transducer platform, bioanalytical principles (affinity or kinetic), and biorecognition elements origin/properties (i.e. antibodies, enzymes, cells, aptamers, etc.), and (ii) the application area. This review follows the latter strategy and focuses on the applications. This allows discussion on how different sensing strategies are brought to bear on the same problem and highlights advantages/disadvantages of these sensing strategies. Given the broad range of biosensor related applications, several particularly relevant areas of application were selected for review: biological threat agents, chemical threat agents, and medicine.

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Section: Salmonellamentioning

confidence: 99%

Biosensor technology: recent advances in threat agent detection and medicine

et al. 2013

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“…Because of this, there is a critical need to develop and improve novel diagnostic techniques that are cost effective and relatively easy to employ in the field. Recently, biosensors have been used for the detection of a wide variety of analytes for various applications 28–33 . Biosensors can be used for health care‐related applications, such as the detection of microorganisms for disease diagnostics 34 .…”

Section: Discussionmentioning

confidence: 99%

Development of a novel immunoFET technology‐based POC assay for detection of Leishmania donovani and Leishmania major

Doni

Bertani

Volpedo

et al. 2023

Parasite Immunology

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Leishmaniasis is considered as one of the 20 neglected tropical diseases. Current methods of leishmanial diagnosis depend on conventional laboratory-based techniques, which are time-consuming, costly and require special equipment and trained personnel. In this context, we aimed to provide an immuno field effect transistors (ImmunoFET) biosensor that matches the conventional standards for point-of-care (POC) monitoring and detection of Leishmania (L.) donovani/Leishmania major. Crude antigens prepared by repeated freeze thawing of L. donovani/L. major stationary phase promastigotes were used for ELISA and ImmunoFETs. Lesishmania-specific antigens were serially diluted in 1Â PBS from a concentration of 10 6 -10 2 parasites/ mL. A specific polyclonal antibody-based sandwich ELISA was established for the detection of Leishmania antigens. An immunoFET technology-based POC novel assay was constructed for the detection of Leishmania antigens. Interactions between antigen-antibody at the gate surface generate an electrical signal that can be measured by semiconductor field-effect principles. Sensitivity was considered and measured as the change in current divided by the initial current. The final L. donovani/ L. major crude antigen protein concentrations were measured as 1.50 mg/mL. Sandwich ELISA against the Leishmania 40S ribosomal protein detected Leishmania antigens could detect as few as 100 L. donovani/L. major parasites. An immunoFET biosensor was constructed based on the optimization of aluminium gallium nitride/ gallium nitride (AlGaN/GaN) surface oxidation methods. The device surface was composed by an AlGaN/GaN wafer with a 23 nm AlGaN barrier layer, a 2 μm GaN layer on the silicon carbide (SiC) substrate for Leishmania binding, and coated with a specific antibody against the Leishmania 40S ribosomal protein, which was successfully detected at concentrations from 10 6 to 10 2 parasites/mL in 1Â PBS. At the concentration of 10 4 parasites, the immunoFETs device sensitivities were 13% and 0.052% in the sub-threshold regime and the saturation regime, respectively. Leishmania parasites were successfully detected by the ImmunoFET biosensor at a diluted concentration as low as 150 ng/mL. In this study, the developed ImmunoFET biosensor performed well. ImmunoFET biosensors can be used as an alternative diagnostic

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“…In this regard, hybridization based electrical DNA detection techniques implementing field-effect transistors (FET) are very convenient for diagnostic and comparative sequencing. [3][4][5][6] Aluminum gallium nitride/gallium nitride (AlGaN/GaN) heterostructure with their superior material characteristics such as non-toxicity to the living cells, higher chemical and thermal stability, higher sensitivity, higher energy bandgap, etc. presents an attractive platform for the fabrication of the FET based biosensor.…”

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“…Among many strategies for the probe DNA immobilization, attachment of thiolated probe DNA on the gold gate electrode of the FET has been used widely. [3][4][5][6]9,10 Though very sensitive, this immobilization chemistry involves the substantial rearrangement of DNA film structure (possibly changing from virtually flat to the tethering by one or two bases, in addition to the thiol-Au bonding on the Au gate electrode of the FET (Ref. 11)), thereby arbitrarily changing the surface potential over the time.…”

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confidence: 99%

“…11)), thereby arbitrarily changing the surface potential over the time. Kang et al 4 reported the increase in the drain current while Wang and Lu 12 and Alur et al 6 showed the decrease in the drain current upon immobilization of the thiolated probe on the HEMT. In addition, this approach potentially presents higher possibility of nonspecific binding due to the charged biomolecules interacting very closely with metal surface.…”

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confidence: 99%

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Biofunctionalized AlGaN/GaN high electron mobility transistor for DNA hybridization detection

Thapa

Alur

Kim

et al. 2012

Applied Physics Letters

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Label-free electrical detection of deoxyribonucleic acid (DNA) hybridization was demonstrated using an AlGaN/GaN high electron mobility transistor (HEMT) based transducer with a biofunctionalized gate. The HEMT DNA sensor employed the immobilization of amine-modified single strand DNA on the self-assembled monolayers of 11-mercaptoundecanoic acid. The sensor exhibited a substantial current drop upon introduction of complimentary DNA to the gate well, which is a clear indication of the hybridization. The application of 3 base-pair mismatched target DNA showed little change in output current characteristics of the transistor. Therefore, it can be concluded that our DNA sensor is highly specific to DNA sequences. V

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DNA hybridization sensor based on AlGaN/GaN HEMT

Cited by 7 publications

References 8 publications

Biosensor technology: recent advances in threat agent detection and medicine

Biosensor technology: recent advances in threat agent detection and medicine

Development of a novel immunoFET technology‐based POC assay for detection of Leishmania donovani and Leishmania major

Biofunctionalized AlGaN/GaN high electron mobility transistor for DNA hybridization detection

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