DNA hypomethylation concomitant with the overproduction of ROS induced by naphthoquinone juglone on tobacco BY-2 suspension cells

Pobořilová, Zuzana; Ohlsson, Anna B.; Berglund, Torkel; Vildova, Alena; Provazník, Ivo; Babula, Petr

doi:10.1016/j.envexpbot.2015.01.005

Cited by 26 publications

(14 citation statements)

References 90 publications

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“…In growth analyses, the treatment usually lasted several weeks and the effect was harmful [19,21,28]. In experiments in which the physiological response or gene expression was investigated, the treatment with juglone lasted only hours [3,4,29,30]. Therefore, it is difficult to compare our results with most of the literature data.…”

Section: Discussioncontrasting

confidence: 52%

Morpho-histological analysis of tomato (Solanum lycopersicum L.) plants after treatment with juglone

et al. 2017

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Juglone is a substance that limits plant growth and has a toxic effect on plant development. In this study, we analyzed the influence of juglone at two different concentrations (10 −3 M and 10 −4 M), which were applied to different parts of Solanum lycopersicum L. plants (root system, stem after decapitation, and surface of a younger leaf or after autografting) for a short period of time (7 days), on the morphology and histology of stems. At a lower concentration, juglone had positive effects on plant growth, which resulted in an increase in interfascicular cambial cell divisions, faster development of a continuous cambium layer along the stem circumference, and development of fibers. Additionally, under the influence of juglone, the number of developing leaves increased and adventitious roots developed. The results are discussed based on the current literature concerning the reaction of plants to juglone and to stress conditions.

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Section: Discussioncontrasting

confidence: 52%

Morpho-histological analysis of tomato (Solanum lycopersicum L.) plants after treatment with juglone

et al. 2017

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“…These results indicate a crucial role of interspecies differences in plant response to the presence of juglone and differences in the reaction of a given species depending on the used concentration of this allelochemical. It has been reported that the investigated allelochemical induced circumstantial disturbances in cell division, elongation processes, and cell death [16,[29][30]. Terzi and Kocaçalişkan [15] revealed that exogenous application of JU (1 mM) markedly declined the length of wheat roots and shoots by 80% and 62%, respectively.…”

Section: Discussionmentioning

confidence: 99%

Juglone-Triggered Oxidative Responses in Seeds of Selected Cereal Agrosystem Plant Species

Sytykiewicz¹,

Kozak²,

Łukasik³

et al. 2019

Pol. J. Environ. Stud.

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Juglone (JU; 5-hydroxy-1,4-naphthoquinone) is an allelochemical synthetized by a wide range of Juglandaceae members. This compound evokes a wide array of detrimental effects in susceptible acceptor plants, including growth inhibition and impairments in the course of biochemical and physiological processes. Therefore, juglone may be considered as a potential bioherbicide in weed control in cereal crops. The purpose of our study was to determine the influence of JU treatment on the content of superoxide anion radical (O 2 •−)-sensitive and early marker of oxidative stress, and the activity of superoxide dismutase (SOD)-enzyme that functions as a crucial component of the first line antioxidative defense in seed tissues. In the study we tested seeds of four selected plant species of cereal agrosystem: corn poppy (Papaver rhoeas L.), corn cockle (Agrostemma githago L.), spring wheat (Triticum aestivum L., cv. Nawra), and spring oat (Avena sativa L., cv. Maczo). Additionally, the allelopathic influence of JU on seed germination of the studied plant species was investigated. The seeds were exposed to three examined juglone concentrations: 0.001, 0.1, and 10 mM (4% methanoldeionized water solutions), whereas the control seeds were treated with aqueous methanolic solutions (4%). Obtained results evidenced much more severe suppression of germination process and robust generation of superoxide anion radical in the tested weeds, compared to the cereals, and the crucial role of superoxide dismutase in limiting the juglone-induced oxidative stress in the studied seeds.

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“…The production of ROS, especially of hydroxyl radicals, was determined by 2´,7´-dichlorodihydrofluorescein diacetate (H 2 DCF DA, 492–495/517–527 nm, Life Technologies, Carlsbad, CA, USA), in compliance with a procedure described by Poborilova et al [ 49 ]. 3′-(p-aminophenyl) fluorescein (APF, 490/515 nm, Life Technologies, Carlsbad, CA, USA) was used to visualize hydroxyl radicals, peroxynitrite and hypochlorite anions.…”

Section: Methodsmentioning

confidence: 99%

“…There are many studies that use the BY-2 cells to evaluate toxic effects of heavy metals, different types of chemicals, pharmaceuticals and also different types of nanoparticles [ 45 , 46 , 47 , 48 ]. One of the most recent works used BY-2 cells to evaluate the phytotoxicity of naphthoquinones, mainly in connection with reactive oxygen species and changes in DNA methylation [ 49 ]. BY-2 cells are also easily cultivated in laboratory.…”

Section: Introductionmentioning

confidence: 99%

Zinc Oxide Nanoparticles Damage Tobacco BY-2 Cells by Oxidative Stress Followed by Processes of Autophagy and Programmed Cell Death

2020

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Nanomaterials, including zinc oxide nanoparticles (ZnO NPs), have a great application potential in many fields, such as medicine, the textile industry, electronics, and cosmetics. Their impact on the environment must be carefully investigated and specified due to their wide range of application. However, the amount of data on possible negative effects of ZnO NPs on plants at the cellular level are still insufficient. Thus, we focused on the effect of ZnO NPs on tobacco BY-2 cells, i.e., a widely accepted plant cell model. Adverse effects of ZnO NPs on both growth and biochemical parameters were observed. In addition, reactive oxygen and nitrogen species visualizations confirmed that ZnO NPs may induce oxidative stress. All these changes were associated with the lipid peroxidation and changes in the plasma membrane integrity, which together with endoplasmatic reticulum and mitochondrial dysfunction led to autophagy and programmed cell death. The present study demonstrates that the phytotoxic effect of ZnO NPs on the BY-2 cells is very complex and needs further investigation.

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DNA hypomethylation concomitant with the overproduction of ROS induced by naphthoquinone juglone on tobacco BY-2 suspension cells

Cited by 26 publications

References 90 publications

Morpho-histological analysis of tomato (Solanum lycopersicum L.) plants after treatment with juglone

Morpho-histological analysis of tomato (Solanum lycopersicum L.) plants after treatment with juglone

Juglone-Triggered Oxidative Responses in Seeds of Selected Cereal Agrosystem Plant Species

Zinc Oxide Nanoparticles Damage Tobacco BY-2 Cells by Oxidative Stress Followed by Processes of Autophagy and Programmed Cell Death

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