DNA integrity, growth pattern, spindle formation, chromosomal constitution and imprinting patterns of mouse oocytes from vitrified pre-antral follicles

Trapphoff, Tom; Hajj, Nady El; Zechner, Ulrich; Haaf, Thomas; Eichenlaub-Ritter, Ursula

doi:10.1093/humrep/deq278

Cited by 97 publications

(85 citation statements)

References 107 publications

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“…Using long-term IVM and supraphysiological recombinant FSH (rFSH), imprinted methylation perturbations were observed in pools of GV oocytes with H19 GOM (1/7 pools), Peg1 LOM (1/7 pools), and Igf2r LOM (6/7 pools) (Kerjean et al 2003; Table 2). By comparison, normal H19 (0/39 strands) and Igf2r methylation (0/15 strands) was found in pooled GV oocytes following longterm IVM with physiological rFSH, although LOM was observed at Snrpn (2/47 DNA strands) (Trapphoff et al 2010). Additionally, independent studies with long-term IVM using physiological or supraphysiological rFSH reported normal Snrpn, Igf2r, Peg3, and H19 methylation in MII oocyte pools (Anckaert et al 2009a(Anckaert et al , 2009b(Anckaert et al , 2010.…”

Section: In Vitro Oocyte Maturationmentioning

confidence: 90%

“…Ultra-rapid vitrification of mouse preantral follicles followed by long-term IVM to GV oocytes led to Snrpn LOM (1/50 strands) but not Igf2r LOM (0/15 strands) or H19 GOM (0/58 strands) in pooled vitrified oocytes (Trapphoff et al 2010). In the second study, mice produced following whole ovary cryopreservation maintained normal H19 and Kcnq1ot1 methylation ratios (Sauvat et al 2008).…”

Section: Analysis Of Imprinting Stability During Artsmentioning

confidence: 94%

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Genomic imprints as a model for the analysis of epigenetic stability during assisted reproductive technologies

Denomme¹,

Mann²

2012

Reproduction

112

118

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Gamete and early embryo development are important stages when genome-scale epigenetic transitions are orchestrated. The apparent lack of remodeling of differential imprinted DNA methylation during preimplantation development has lead to the argument that epigenetic disruption by assisted reproductive technologies (ARTs) is restricted to imprinted genes. We contend that aberrant imprinted methylation arising from assisted reproduction or infertility may be an indicator of more global epigenetic instability. Here, we review the current literature on the effects of ARTs, including ovarian stimulation, in vitro oocyte maturation, oocyte cryopreservation, IVF, ICSI, embryo culture, and infertility on genomic imprinting as a model for evaluating epigenetic stability. Undoubtedly, the relationship between impaired fertility, ARTs, and epigenetic stability is unquestionably complex. What is clear is that future studies need to be directed at determining the molecular and cellular mechanisms giving rise to epigenetic errors.

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Section: In Vitro Oocyte Maturationmentioning

confidence: 90%

Section: Analysis Of Imprinting Stability During Artsmentioning

confidence: 94%

Genomic imprints as a model for the analysis of epigenetic stability during assisted reproductive technologies

Denomme¹,

Mann²

2012

Reproduction

112

118

View full text Add to dashboard Cite

show abstract

“…Some studies have demonstrated that vitrification does not significantly alter gene methylation patterns in oocytes [110,115,119] and blastocysts [112]. In contrast, vitrification has been found to reduce gene methylation in mouse oocytes [113], embryos [112], and fetuses [117].…”

Section: Molecular Effects Of Cryopreservationmentioning

confidence: 99%

Cryopreservation of mammalian oocytes and embryos: current problems and future perspectives

Moussa

Shen

Zhang

et al. 2014

Sci. China Life Sci.

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Cryopreservation techniques for mammalian oocytes and embryos have rapidly progressed during the past two decades, emphasizing their importance in various assisted reproductive technologies. Pregnancies and live births resulting from cryopreserved oocytes and embryos of several species including humans have provided proof of principle and led to the adoption of cryopreservation as an integral part of clinical in vitro fertilization. Considerable progress has been achieved in the development and application of the cryopreservation of mammalian oocytes and embryos, including preservation of the reproductive potential of patients who may become infertile, establishment of cryopreserved oocyte banks, and transport of oocytes and embryos internationally. However, the success rates are still far lower than those obtained with fresh oocytes and embryos, and there are still obstacles that need to be overcome. In this review, we address the major obstacles in the development of effective cryopreservation techniques. Such knowledge may help to eliminate these hurdles by revealing which aspects need improvement. Furthermore, this information may encourage further research by cryobiologists and increase the practical use of cryopreservation as a major part of assisted reproductive technologies for both humans and animal species. cryopreservation, mammals, oocytes, embryos, cryoinjuries Citation:Moussa M, Shu J, Zhang XH, Zeng FY. Cryopreservation of mammalian oocytes and embryos: current problems and future perspectives. Sci China Life Sci, 2014, 57: 903 -914,

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“…We have developed two different multiplex assays, each with four genes, for studying imprinted gene methylation in the murine 25 and bovine model, 26 respectively. Outer …”

confidence: 99%

Limiting dilution bisulfite (pyro)sequencing reveals parent-specific methylation patterns in single early mouse embryos and bovine oocytes

et al. 2011

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DNA integrity, growth pattern, spindle formation, chromosomal constitution and imprinting patterns of mouse oocytes from vitrified pre-antral follicles

Abstract: DNA breaks appear to be rapidly repaired and vitrification of oocytes inside pre-antral follicles by the Cryo-Top method does not appear to increase risks of abnormal imprinting or disturbances in spindle formation and chromosome segregation.

Cited by 97 publications

References 107 publications

Genomic imprints as a model for the analysis of epigenetic stability during assisted reproductive technologies

Genomic imprints as a model for the analysis of epigenetic stability during assisted reproductive technologies

Cryopreservation of mammalian oocytes and embryos: current problems and future perspectives

Limiting dilution bisulfite (pyro)sequencing reveals parent-specific methylation patterns in single early mouse embryos and bovine oocytes

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