DNA looping-mediated repression by histone-like protein H-NS: specific requirement of Eσ<sup>70</sup> as a cofactor for looping

Shin, Minsang; Song, Mi-Ryoung; Rhee, Joon Haeng; Hong, Yeongjin; Kim, You-Jin; Seok, Yeong‐Jae; Ha, Kwon‐Soo; Jung, Se-Hui; Choy, Hyon E.

doi:10.1101/gad.1316305

Cited by 149 publications

(114 citation statements)

References 66 publications

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“…ment with a previous report (39). When the assays were performed using late-stationary-phase cells (overnight cultures), the activity found was about 10-fold higher than that in exponential-phase cells (data not shown).…”

Section: Vol 190 2008supporting

confidence: 62%

“…Regulation of the hdeAB operon. The figure has been produced using data from the literature (8,13,16,20,22,24,26,27,30,33,37,39,42,43,44,46) as well as the results obtained in this study. Thick arrows represent genes.…”

Section: Resultsmentioning

confidence: 99%

“…In parentheses are the number of nucleotides that match the consensus sequence/number of nucleotides of the consensus sequence. Even though hdeAB is transcribed from RNA polymerase containing 70 or 38 (39), its promoter does not have the Ϫ10 sequence (CTATACT) found in many promoters regulated by 38 that replaces the Ϫ10 sequence (XTATAAT) found in 70 promoters (12). TSS, transcription start site.…”

Section: Vol 190 2008mentioning

confidence: 99%

“…hdeAB REPRESSION BY MarA 1291 have a major effect on the expression of hdeAB (exponential versus stationary phase and pH 7.0 versus pH 5.5 [19,39,43,44]) and on the physiology of the HdeA and HdeB proteins (LB rich medium [28]). In a ⌬marRAB strain bearing an hdeABp-lacZ chromosomal reporter, the expression of hdeAB at pH 7.0 increased 1.7-fold from exponential phase to early stationary phase (Fig.…”

Section: Vol 190 2008mentioning

confidence: 99%

“…The hdeAB operon is induced in stationary phase (39,44) and at acid pH (19,43). Transcription of hdeAB depends on complex circuits of regulation involving RpoD ( 70 ), RpoS ( 38 ), H-NS, cyclic AMP receptor protein, GadE, GadX, GadW, GadY, EvgAS, YdeO, Lrp, MarA, SoxRS, TorRS, and TrmE, among other regulators (see Fig.…”

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confidence: 99%

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Role of the Multidrug Resistance Regulator MarA in Global Regulation of the hdeAB Acid Resistance Operon in Escherichia coli

2008

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MarA, a transcriptional regulator in Escherichia coli, affects functions such as multiple-antibiotic resistance (Mar) and virulence. Usually an activator, MarA is a repressor of hdeAB and other acid resistance genes. We found that, in wild-type cells grown in LB medium at pH 7.0 or pH 5.5, repression of hdeAB by MarA occurred only in stationary phase and was reduced in the absence of H-NS and GadE, the main regulators of hdeAB. Moreover, repression of hdeAB by MarA was greater in the absence of GadX or Lrp in exponential phase at pH 7.0 and in the absence of GadW or RpoS in stationary phase at pH 5.5. In turn, MarA enhanced repression of hdeAB by H-NS and hindered activation by GadE in stationary phase and also reduced the activity of GadX, GadW, RpoS, and Lrp on hdeAB under some conditions. As a result of its direct and indirect effects, overexpression of MarA prevented most of the induction of hdeAB expression as cells entered stationary phase and made the cells sevenfold more sensitive to acid challenge at pH 2.5. These findings show that repression of hdeAB by MarA depends on pH, growth phase, and other regulators of hdeAB and is associated with reduced resistance to acid conditions.

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Section: Vol 190 2008supporting

confidence: 62%

Section: Resultsmentioning

confidence: 99%

Section: Vol 190 2008mentioning

confidence: 99%

Section: Vol 190 2008mentioning

confidence: 99%

mentioning

confidence: 99%

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Role of the Multidrug Resistance Regulator MarA in Global Regulation of the hdeAB Acid Resistance Operon in Escherichia coli

2008

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Biofilm Development and Stress Response in the Cholera Bacterium

Silva

Benítez

2016

Stress and Environmental Regulation of Gene Expression and Adaptation in Bacteria

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Probing bacterial nucleoid structure with optical tweezers

Dorman

2007

BioEssays

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The H-NS protein is a major component of the nucleoid in Gram-negative bacterial cells. It is a global regulator of transcription that affects the expression of many genes, including virulence genes in pathogenic species. At a local level, it facilitates the formation of nucleoprotein structures that repress transcriptional promoter function. H-NS can form bridges between different DNA molecules or between different sections of the same molecule, allowing it to compact and impose structure on the nucleoid. A recent paper by Dame et al.1 reports new insights into H-NS-mediated DNA bridging that were obtained using an optical tweezers device.

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DNA looping-mediated repression by histone-like protein H-NS: specific requirement of Eσ⁷⁰ as a cofactor for looping

Cited by 149 publications

References 66 publications

Role of the Multidrug Resistance Regulator MarA in Global Regulation of the hdeAB Acid Resistance Operon in Escherichia coli

Role of the Multidrug Resistance Regulator MarA in Global Regulation of the hdeAB Acid Resistance Operon in Escherichia coli

Biofilm Development and Stress Response in the Cholera Bacterium

Probing bacterial nucleoid structure with optical tweezers

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DNA looping-mediated repression by histone-like protein H-NS: specific requirement of Eσ70 as a cofactor for looping

Cited by 149 publications

References 66 publications

Role of the Multidrug Resistance Regulator MarA in Global Regulation of the hdeAB Acid Resistance Operon in Escherichia coli

Role of the Multidrug Resistance Regulator MarA in Global Regulation of the hdeAB Acid Resistance Operon in Escherichia coli

Biofilm Development and Stress Response in the Cholera Bacterium

Probing bacterial nucleoid structure with optical tweezers

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DNA looping-mediated repression by histone-like protein H-NS: specific requirement of Eσ⁷⁰ as a cofactor for looping