DNA-mediated assembly of a gold-nanoparticle film with controllable sonic behavior detected by novel electric-induced ultrasound

Abstract

Two-dimensional gold assembled nanomaterials have garnered considerable interests in biomedical application such as wearable sensors and flexible devices. The assembly can be accomplished via attractive interactions between gold nanoparticles (GNPs)...

Single-virus-sensitive barcode qPCR mediated by the aggregation of gold nanoparticle probes

“…[3][4][5] However, when analysing throat swab samples from infected people within 1 day of viral infection, it is often difficult to obtain accurate results using qPCR due to the presence of only a few viral particles. 6,7 Gold nanoparticles (GNPs) show advantages such as being readily available and robust and displaying excellent biocompatibility and cost-effectiveness, 8 and, they have demonstrated high sensitivity and specificity [9][10][11][12] in the detection of DNA in biological species. To this end, based on our previous research on DNA-functionalized GNPs for DNA detection [13][14][15] and the ease of separating GNPs after they aggregate, we developed a GNP-mediated barcode qPCR strategy with sensitivity for a single virus particle for the detection of influenza virus H3N2, a single-stranded RNA virus like COVID-19.…”