DNA methylation and mRNA expression of COL6A3 in antler mesenchyme of female and male reindeer

Zhai, Jiancheng; Han, Ruobing; Wang, Shengnan; Wang, Qiang‐Hui; Xia, Yanling; Liu, Weishi; Yin, Yulong; Li, Heping

doi:10.1007/s13258-019-00829-3

Cited by 5 publications

(3 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Genomic studies in Arabidopsis reveal that many endogenous genes are methylated in their promoter or coding regions, and gene methylation levels are strongly correlated with transcription levels (Vaughn et al, 2007;Cokus et al, 2008). DNA methylation occurs mainly in the CpG island-rich promoter region, which may hinder the binding of transcription factors to the promoter, thereby inhibiting gene transcription (Zhai et al, 2019). The methylation pattern of exogenous genes reveals that the methylation rate of the CG site of the Gus promoter (−280 to −85 region) is 0-14.28% and that of the CHG sites is 33.33-66.67%.…”

Section: Cytosine Methylation Patterns and Stability Of Exogenous Genesmentioning

confidence: 99%

DNA Methylation Silences Exogenous Gene Expression in Transgenic Birch Progeny

Chen

Yin

et al. 2020

Front. Plant Sci.

View full text Add to dashboard Cite

The genetic stability of exogenous genes in the progeny of transgenic trees is extremely important in forest breeding; however, it remains largely unclear. We selected transgenic birch (Betula platyphylla) and its hybrid F1 progeny to investigate the expression stability and silencing mechanism of exogenous genes. We found that the exogenous genes of transgenic birch could be transmitted to their offspring through sexual reproduction. The exogenous genes were segregated during genetic transmission. The hybrid progeny of transgenic birch WT1×TP22 (184) and WT1×TP23 (212) showed higher Bgt expression and greater insect resistance than their parents. However, the hybrid progeny of transgenic birch TP23×TP49 (196) showed much lower Bgt expression, which was only 13.5% of the expression in its parents. To elucidate the mechanism underlying the variation in gene expression between the parents and progeny, we analyzed the methylation rates of Bgt in its promoter and coding regions. The hybrid progeny with normally expressed exogenous genes showed much lower methylation rates (0–29%) than the hybrid progeny with silenced exogenous genes (32.35–45.95%). These results suggest that transgene silencing in the progeny is mainly due to DNA methylation at cytosine residues. We further demonstrated that methylation in the promoter region, rather than in the coding region, leads to gene silencing. We also investigated the relative expression levels of three methyltransferase genes: BpCMT, BpDRM, and BpMET. The transgenic birch line 196 with a silenced Gus gene showed, respectively, 2.54, 9.92, and 4.54 times higher expression levels of BpCMT, BpDRM, and BpMET than its parents. These trends are consistent with and corroborate the high methylation levels of exogenous genes in the transgenic birch line 196. Therefore, our study suggests that DNA methylation in the promoter region leads to silencing of exogenous genes in transgenic progeny of birch.

show abstract

Section: Cytosine Methylation Patterns and Stability Of Exogenous Genesmentioning

confidence: 99%

DNA Methylation Silences Exogenous Gene Expression in Transgenic Birch Progeny

Chen

Yin

et al. 2020

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…The methylation of Sca-1 promoter was detected by bisulfite sequencing PCR (BSP) 28 . Briefly, genome DNA extraction kit (Takara, Japan) was used to extract genome DNA from EPCs.…”

Section: Methylation Of Sca-1 Promotermentioning

confidence: 99%

Cigarette smoke extract affects methylation status and attenuates Sca-1 expression of mouse endothelial progenitor cell <i>in vitro</i>

He¹,

Chen²,

Chen³

et al. 2021

Tob. Induc. Dis.

View full text Add to dashboard Cite

INTRODUCTION Endothelial dysfunction appears in many smoking-related diseases, it is also an important pathophysiological feature. Endothelial progenitor cells (EPCs) are precursors of endothelial cells and have a crucial effect on the repair and maintenance of endothelial integrity. Sca-1 is not only common in bone marrow-derived hematopoietic stem cells (HSCs), but it is also expressed in nonhematopoietic organs by tissue-resident stem and progenitor cells. The aim of this study is to investigate the impact of cigarette smoke extract (CSE) on the function of bone marrow-derived EPCs and the expression level of Sca-1 in EPCs, and also whether the methylation of Sca-1 is involved in EPC dysfunction. METHODS We measured EPC capacities including adhesion, secretion and proliferation, the concentration of endothelial nitric oxide synthase (eNOS) and apoptosis-inducing factor (AIF) in cell culture supernatant, and also Sca-1 expression and promoter methylation in EPCs induced by CSE. Decitabine (Dec) was applied to test whether it could alter the impact caused by CSE. RESULTS The adhesion, proliferation and secretion ability of EPCs can be induced to be decreased by CSE in vitro , accompanied by decreased concentrations of AIF and eNOS in cell culture supernatant and decreased Sca-1 expression in EPCs. In addition, Dec could partly attenuate the impact described above. There were no significant differences in the quantitative analysis of Sca-1 promoter methylation among different groups. CONCLUSIONS The decreased Sca-1 expression was related to EPC dysfunction induced by CSE. EPC dysfunction resulting from CSE may be related to methylation mechanism, but not the methylation of Sca-1 promoter.

show abstract

“…Ectopic expression of E2F1 increases the LMCD1-AS1 level [34]. Alpha 3(VI) collagen gene (COL6A3) [35] is an extracellular matrix protein [36] that forms a microfibrillar network associated with the structural integrity [35] and is usually found in most connective tissues, including muscle, skin, tendon and vessels [36]. COL6A3 is strongly related to many diseases [37].…”

Section: Lmcd1-as1 In Cholangiocarcinomamentioning

confidence: 99%

LMCD1 antisense RNA 1 is a newly identified long noncoding RNA

Yalman

2021

Anti-Cancer Drugs

View full text Add to dashboard Cite

Long noncoding RNAs (lncRNAs) are one of the interesting fields in cancer researches. LncRNAs are generally dysregulated in many diseases. LMCD1 antisense RNA 1 (LMCD1-AS1) is a newly identified lncRNA with protumorigenic functions on tumor cells. LMCD1-AS1 expression is increased in hepatocellular carcinoma (HCC). LMCD1-AS1 is a sponge of miR-106b-5p activity. LMCD1-AS1 modulates the survival of osteosarcoma via targeting miR-106b-5p. LMCD1-AS1 and Sp1 are highly expressed in osteosarcoma. SP1 can bind to the promoter region of LMCD1-AS1, resulting in its overexpression in osteosarcoma. GLI2 is shown to bind to the LMCD1-AS1 promoter and is transcriptionally activated by LMCD1-AS1. LMCD1 acts as a miR-1287-5p sponge to increase GLI2 expression. LMCD1 is abundantly expressed in kidney tissue. Moreover, it is functionally involved in protein-protein interactions with transcriptional co-repressor activity, including regulation of the calcineurin-NFAT signaling cascade known to play a critical role in recovery from acute kidney injury (AKI). The E2F1/LMCD1-AS1/miR-345-5p/COL6A3 axis is a newly identified regulatory mechanism, which has a function in cholangiocarcinoma (CCA) tumorigenesis and progression and provides potential therapeutic targets for CCA. Also, LMCD1-AS1 functions in thyroid cancer (THCA) development. LMCD1-AS1 is overexpressed in THCA cells, and LMCD1-AS1 knockdown suppresses the malignant phenotypes of THCA cells. In THCA development, LMCD1-AS1 exerts protumorigenic function through sponging miR-1287-5p to increase GLI2 expression, constituting a feedback loop of LMCD1-AS1/miR-1287-5p/ GLI2. In this review, the author focuses on the molecular mechanisms of newly identified long noncoding RNA LMCD1 antisense RNA 1 (LMCD1-AS1). Anti-Cancer Drugs

show abstract

DNA methylation and mRNA expression of COL6A3 in antler mesenchyme of female and male reindeer

Cited by 5 publications

References 34 publications

DNA Methylation Silences Exogenous Gene Expression in Transgenic Birch Progeny

DNA Methylation Silences Exogenous Gene Expression in Transgenic Birch Progeny

Cigarette smoke extract affects methylation status and attenuates Sca-1 expression of mouse endothelial progenitor cell <i>in vitro</i>

LMCD1 antisense RNA 1 is a newly identified long noncoding RNA

Contact Info

Product

Resources

About