DNA methylation‐derived systemic inflammation indices and their association with oropharyngeal cancer risk and survival

Abstract

Background Head and neck squamous cell carcinomas are associated with systemic inflammation (SI). We evaluated whether DNA methylation‐derived SI (mdSI) indices are associated with oropharyngeal cancer risk and survival. Methods Ninety‐four oropharyngeal squamous cell carcinoma (OPSCC) cases and 57 controls with DNA methylation data were included. Logistic regression analysis and survival analysis were performed to test the association of mdSI indices with OPSCC risk and survival. Results Higher methylation‐derived neutrophil‐to‐lymphocyte ratio (mdNLR) was associated with increased risk of OPSCC (OR = 1.21, 95%CI: 1.11–1.40) while no association was found with methylation‐derived lymphocyte‐to‐monocyte ratio (mdLMR). For 5‐year overall survival, higher mdLMR was significantly associated with decreased risk of death (HR = 0.25, 95%CI: 0.10–0.64) while the converse was observed for mdNLR (HR = 2.48, 95%CI: 1.04–5.92). Conclusion We observed an association between mdSI indices and OPSCC risk and 5‐year overall survival. It is possible to use mdLMR as an independent prognostic factor for OPSCC.

Hypermethylation of nc886 in HPV-positive oropharyngeal cancer and its clinical implications: An epigenome-wide association study

“…The patients in the external validation stage were derived from a case control study of HNSCC in the greater Boston area, and the patient recruitment and data collection process was described in detail previously. 65 , 66 This study was approved by the institutional review board of MDACC. All patients provided informed consent.…”

“…The whole-epigenome CpG site methylation profiling for both the discovery and the internal and external validation stages was performed using Illumina human MethylationEPIC BeadChip as previously described. 66 , 67 The MethylationEPIC BeadChip contains more than 850,000 CpG sites across the human epigenome, which is enriched for CpG sites located in gene promoter regions (54%), gene bodies (30%), and CpG islands (19%). 68 Briefly, genomic DNA was treated with sodium bisulfite using the EZ DNA Methylation-Gold Kit (Zymo Research, Irvine, CA) and hybridized to the BeadChips.…”

The Prognostic Role of miR-31 in Head and Neck Squamous Cell Carcinoma: Systematic Review and Meta-Analysis with Trial Sequential Analysis

“…At the base of the etiopathogenetic processes of cancerization, we found genetic alterations that involve mutations of the main oncogenes (APC, p53, NF1, VHL, Rb BCL2, BRCA2, PTCH CD95, ST5, SWI/SNF, p16, YPEL3, ST7, and ST14) and oncogenes (Ras, raf, gsp, jun, fas, erbA, abl, sis erbB fms). In addition, events affecting cell cycle progression and proliferation, such as DNA methylation [7], histone modifications, and non-coding alterations of RNA [8] (such as microRNAs), can influence, cancer progression, stemness and resistance of cancer cells to therapeutics [9][10][11][12][13][14][15][16][17][18].…”