DNA methylation is associated with expression level changes of galectin gene in mantle wound healing process of pearl oyster, Pinctada fucata

Li, Yaoguo; Huang, Xiande; Guan, Yunyan; Shi, Yu; Zhang, Hua; He, Maoxian

doi:10.1016/j.fsi.2015.06.016

Cited by 22 publications

(6 citation statements)

References 39 publications

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“…Our study showed a relatively low level (12.0%–32.6%) of the total methylation in comparison with other organisms, whose methylation levels were estimated using the same method: 70% in Hordeum vulgare , 15.49%–46.10% in Arabidopsis thaliana , ~60.0% in Lagopus lagopus scotica , 23.3%–33.4% in Salmo trutta , 55.0%–67.0% in Xenostrobus secures and 63.5%–73.0% in Ficopomatus enigmaticus (Ardura, Zaiko, Morán, Planes, & Garcia‐Vazquez, ; Du & Wang, ; Morán et al., ; Wenzel & Piertney, ). Overall, the frequency of internal C methylation was higher than that of hemimethylation in C. savignyi , which was consistent to the Chinese shrimp ( Fenneropenaeus chinesis ), pearl oyster ( Pinctada fucata ), Zhikong scallop ( Chlamys farreri ) and brown trout ( Salmo trutta ) (He et al., ; Li et al., ; Morán et al., ; Sun et al., ). The higher frequency of internal C methylation suggests that CpG sequence context might be the dominant target of DNA methylation in C. savignyi , which was detected in its congener C. intestinalis (Feng et al., ).…”

Section: Discussionsupporting

confidence: 70%

Rapid response to changing environments during biological invasions: DNA methylation perspectives

Huang

et al. 2017

Molecular Ecology

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Dissecting complex interactions between species and their environments has long been a research hot spot in the fields of ecology and evolutionary biology. The well-recognized Darwinian evolution has well-explained long-term adaptation scenarios; however, "rapid" processes of biological responses to environmental changes remain largely unexplored, particularly molecular mechanisms such as DNA methylation that have recently been proposed to play crucial roles in rapid environmental adaptation. Invasive species, which have capacities to successfully survive rapidly changing environments during biological invasions, provide great opportunities to study molecular mechanisms of rapid environmental adaptation. Here, we used the methylation-sensitive amplified polymorphism (MSAP) technique in an invasive model ascidian, Ciona savignyi, to investigate how species interact with rapidly changing environments at the whole-genome level. We detected quite rapid DNA methylation response: significant changes of DNA methylation frequency and epigenetic differentiation between treatment and control groups occurred only after 1 hr of high-temperature exposure or after 3 hr of low-salinity challenge. In addition, we detected time-dependent hemimethylation changes and increased intragroup epigenetic divergence induced by environmental stresses. Interestingly, we found evidence of DNA methylation resilience, as most stress-induced DNA methylation variation maintained shortly (~48 hr) and quickly returned back to the control levels. Our findings clearly showed that invasive species could rapidly respond to acute environmental changes through DNA methylation modifications, and rapid environmental changes left significant epigenetic signatures at the whole-genome level. All these results provide fundamental background to deeply investigate the contribution of DNA methylation mechanisms to rapid contemporary environmental adaptation.

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Section: Discussionsupporting

confidence: 70%

Rapid response to changing environments during biological invasions: DNA methylation perspectives

Huang

et al. 2017

Molecular Ecology

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“…The reaction was performed at 37 °C for 2 min, and 85 °C for 5 min. The following transcriptase digestion reaction, dilution of the mixture, and mass spectra data analysis were similar to the report by Li et al [ 52 ].…”

Section: Methodssupporting

confidence: 79%

Sequence, Expression, and Anti-GCRV Function of the Ferritin from the Grass Carp, Ctenopharyngodon idellus

Xiao

Tang

et al. 2022

IJMS

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Ferritin possesses an immune function to defend against pathogen infection. To elucidate the immunity-protecting roles of ferritin from Ctenopharyngodon idellus (Ciferritin) against virus infection, the cDNA and promoter sequences of Ciferritin were determined, and the correlations between Ciferrtin expressions and promoter methylation levels were analyzed. In addition, the functional role of Ciferrtin on GCRV (grass carp reovirus) infection was assessed. The full-length cDNA of Ciferritin is 1053 bp, consists of a 531 bp open-reading frame, and encodes 176 amino acids. Ciferritin showed the highest sequence identity with the ferritin middle subunit of Mylopharyngodon piceus (93.56%), followed by the subunits of Megalobrama amblycephala and Sinocyclocheilus rhinocerous. Ciferritin contains a conserved ferritin domain (interval: 10–94 aa), and the caspase recruitment domain (CARD) and Rubrerythrin domain were also predicted. In the spleen and kidney, significantly higher Ciferritin expressions were observed at 6, 12, 24, or 168 h post GCRV infection than those in the PBS injection group (p < 0.05). The Ciferrtin expression level in the progeny of maternal-immunized grass carp was significantly higher than that in the progeny of common grass carp (p < 0.05). Ciferritin promoter methylation level in the progeny from common grass carp was 1.27 ± 0.15, and in the progeny of the maternal-immunized group was 1.00 ± 0.14. In addition, methylation levels of “CpG9” and “CpG10” loci were significantly lower in the progeny of maternal-immunized fish than those in the common group. Except for the “CpG5”, methylation levels of all other detected “CpG” loci negatively correlated with Ciferritin expression levels. Furthermore, the total methylation level of “CpG1–10” negatively correlated with the Ciferritin expressions. The Ciferritin expression level was significantly up-regulated, and the VP7 protein levels were significantly reduced, at 24 h post GCRV infection in the Ciferritin over-expression cells (p < 0.05). The results from the present study provide sequence, epigenetic modification and expression, and anti-GCRV functional information of Ciferritin, which provide a basis for achieving resistance to GCRV in grass carp breeding.

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“…These results suggest that the hemocytes might assist the mantle tissue in shell regeneration by accelerating the calcium transportation when stressors were detected. Interestingly, the injurious notching operation also activated some immune genes, for example, galectin, AIF and macroglobulin, which play important roles in wound repair in mollusks 40 – 42 . The cooperative responses to LPS and notching stimulation of these genes related to immunity and calcium metabolism reveal that they may share some points in molecular regulation pathways.…”

Section: Discussionmentioning

confidence: 99%

Hemocytes in the extrapallial space of Pinctada fucata are involved in immunity and biomineralization

Huang

Liu

et al. 2018

Sci Rep

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In bivalves, the mantle tissue secretes organic matrix and inorganic ions into the extrapallial space (EPS) to form the shells. In addition, more and more evidences indicate the participation of hemocytes in shell mineralization, but no direct evidence has been reported that verifies the presence of hemocytes in the EPS, and their exact roles in biomineralization remain uncertain. Here, we identified hemocytes from the EPS of Pinctada fucata. Numerous components involved in cellular and humoral immunity were identified by proteome analysis, together with several proteins involved in calcium metabolism. The hemocytes exerted active phagocytosis and significantly upregulated the expression of immune genes after immune stimulation. A group of granulocytes were found to contain numerous calcium-rich vesicles and crystals, which serve as a calcium pool. During shell regeneration, some genes involved in calcium metabolism are upregulated. Strikingly, most of the shell matrix proteins were absent in the hemocytes, suggesting that they might not be solely responsible for directing the growth of the shell. Taken together, our results provided comprehensive information about the function of hemocytes in immunity and shell formation.

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DNA methylation is associated with expression level changes of galectin gene in mantle wound healing process of pearl oyster, Pinctada fucata

Cited by 22 publications

References 39 publications

Rapid response to changing environments during biological invasions: DNA methylation perspectives

Rapid response to changing environments during biological invasions: DNA methylation perspectives

Sequence, Expression, and Anti-GCRV Function of the Ferritin from the Grass Carp, Ctenopharyngodon idellus

Hemocytes in the extrapallial space of Pinctada fucata are involved in immunity and biomineralization

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