DNA Microarray Analysis of Plant Seeds Irradiated by Active Oxygen Species in Oxygen Plasma

Hayashi, Nobuya; Ono, Reoto; Nakano, Riku; Shiratani, Masaharu; Tashiro, Kosuke; Kuhara, Satoru; Yasuda, Kaori; Hagiwara, Hiroko

doi:10.1615/plasmamed.2016018933

Cited by 19 publications

(26 citation statements)

References 2 publications

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“…These changes by the plasma treatment in the short-term may be due to epigenetics such as regulating DNA cytosine methylation, which inactivates gene expression [74,184].…”

Section: Epigenetics and Geneticsmentioning

confidence: 99%

Mechanisms of Plasma-Seed Treatments as a Potential Seed Processing Technology

2021

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Plasma treatments are currently being assessed as a seed processing technology for agricultural purposes where seeds are typically subjected to pre-sowing treatments to improve the likelihood of timely and uniform germination. The aim of this review is to summarize the hypotheses and present the evidence to date of how plasma treatments affect seeds, considering that there is difficulty in standardizing the methodology in this interdisciplinary field given the plethora of variables in the experimental setup of the plasma device and handling of biological samples. The ever increasing interest for plasma agriculture drives the need for a review dedicated to seeds, which is understandable to an interdisciplinary audience of biologists and plasma physicists. Seeds are the first step of the agricultural cycle and at this stage, the plant can be given the highest probability of establishment, despite environmental conditions, to exploit the genetic potential of the seed. Furthermore, seedlings seem to be too sensitive to the oxidation of plasma and therefore, seeds seem to be the ideal target. This review intentionally does not include seed disinfection and sterilization due to already existing reviews. Instead, a summary of the mechanisms of how plasma may be affecting the seed and its germination and developmental properties will be provided and discussed.

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“…These changes by the plasma treatment in the short-term may be due to epigenetics such as regulating DNA cytosine methylation, which inactivates gene expression [74,184].…”

Section: Epigenetics and Geneticsmentioning

confidence: 99%

Mechanisms of Plasma-Seed Treatments as a Potential Seed Processing Technology

2021

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“…After NTP treatment of seeds, the expression of several genes changed. Hayashi et al [ 26 ] found the upregulation of genes involved in increasing energy production by photosynthesis by upregulating enzymes such as RuBisCO, increasing carbon fixation and production of the plant hormone auxin. Nakano et al [ 27 ] also noted changes in the synthesis of several proteins, but it appears that there were no mutagenic effects on seed embryos in genes associated with plant growth, as the growth increase was not inherited from the first plant generation.…”

Section: Nonthermal Plasma Effects On Seedsmentioning

confidence: 99%

“…It is important to know whether the effects have an impact on seeds and plants of future generations. Hayashi et al [ 26 ] reported that the growth of second-generation plants was similar to that of seeds not treated with NTP, indicating that no mutation of growth-related genes occurred during the NTP treatment of first-generation seeds. Gene expression analysis of Arabidopsis thaliana seeds, however, showed a different gene expression pattern in the second-generation compared to the first-generation seeds.…”

Section: Nonthermal Plasma Effects On Seedsmentioning

confidence: 99%

“…Although authors used a variety of experimental setups, the most common methods for NTP generation used for seed treatment are presented in Figure 2 and are based on atmospheric pressure plasma jet discharge (APPJ) [ 34 , 45 , 46 ], dielectric barrier discharge (DBD) [ 13 , 18 , 19 , 20 , 21 , 23 , 24 , 25 , 28 , 32 , 33 , 36 , 39 , 40 , 42 , 44 ] and radio-frequency discharge (RF) [ 13 , 22 , 26 , 27 , 29 , 37 , 38 , 43 , 47 , 48 , 49 , 50 ]. The type of discharge depends on the frequency of the power source (AC, DC), the ambient gas pressure (low and atmospheric pressure) and the exact shape of the electrodes [ 51 ].…”

Section: Introductionmentioning

confidence: 99%

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Effects of Nonthermal Plasma on Morphology, Genetics and Physiology of Seeds: A Review

Starič

Vogel‐Mikuš

Mozetič

et al. 2020

Plants

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Nonthermal plasma (NTP), or cold plasma, has shown many advantages in the agriculture sector as it enables removal of pesticides and contaminants from the seed surface, increases shelf life of crops, improves germination and resistance to abiotic stress. Recent studies show that plasma treatment indeed offers unique and environmentally friendly processing of different seeds, such as wheat, beans, corn, soybeans, barley, peanuts, rice and Arabidopsis thaliana, which could reduce the use of agricultural chemicals and has a high potential in ecological farming. This review covers the main concepts and underlying principles of plasma treatment techniques and their interaction with seeds. Different plasma generation methods and setups are presented and the influence of plasma treatment on DNA damage, gene expression, enzymatic activity, morphological and chemical changes, germination and resistance to stress, is explained. Important plasma treatment parameters and interactions of plasma species with the seed surface are presented and critically discussed in correlation with recent advances in this field. Although plasma agriculture is a relatively new field of research, and the complex mechanisms of interactions are not fully understood, it holds great promise for the future. This overview aims to present the advantages and limitations of different nonthermal plasma setups and discuss their possible future applications.

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“…Recently, useful effects of plants such as enhancements of germination, growth and antioxidative activity have been investigated, which are induced by active particles in plasmas [1][2][3][4][5][6][7][8][9][10][11]. Enhancements of root and stem lengths and leaf area have been observed [1][2][3][4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Variations in Plant Growth Characteristics due to Oxygen Plasma Irradiation on Leaf and Seed

Hayashi¹,

Yamamoto²

2021

Preprint

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Gene expression variations of plant leaf are investigated by irradiating seed and leaf with oxygen or air plasmas. Enhancement of leaf growth is induced by oxygen plasma irradiation on seeds, which is supported by increased gene expression for protein synthesis, oxidative-reduction reactions and decreased gene expression concerning DNA methylation and histone modification. Suppression of leaf growth is observed by the oxygen plasma, which would be owing to increased gene expression concerning heat shock protein and redox reaction, and decreased expression of photosynthesis and glycoprotein. Also, gene expression variation due to air plasma irradiation is almost same as that of oxygen plasma. Active oxygen species are major factors in both oxygen and air plasmas for the variation of gene expressions in plant.

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DNA Microarray Analysis of Plant Seeds Irradiated by Active Oxygen Species in Oxygen Plasma

Cited by 19 publications

References 2 publications

Mechanisms of Plasma-Seed Treatments as a Potential Seed Processing Technology

Mechanisms of Plasma-Seed Treatments as a Potential Seed Processing Technology

Effects of Nonthermal Plasma on Morphology, Genetics and Physiology of Seeds: A Review

Variations in Plant Growth Characteristics due to Oxygen Plasma Irradiation on Leaf and Seed

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