DNA-Mikroarrays

Niemeyer, Christof M.; Blohm, Dietmar

doi:10.1002/(sici)1521-3757(19991004)111:19<3039::aid-ange3039>3.0.co;2-0

Cited by 51 publications

(15 citation statements)

References 90 publications

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“…The hybridization of oligonucleotides in solution to surface‐immobilized DNA is of crucial importance to microarray‐based genetic diagnostic devices 1–3. Most microarray‐based approaches rely on the labeling of samples with a fluorophore or a radioactive tag.…”

Section: The Dna Sequences and Their Nomenclaturesmentioning

confidence: 99%

Label‐Free Detection of DNA Hybridization at the Nanoscale: A Highly Sensitive and Selective Approach Using Atomic‐Force Microscopy

et al. 2003

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Section: The Dna Sequences and Their Nomenclaturesmentioning

confidence: 99%

Label‐Free Detection of DNA Hybridization at the Nanoscale: A Highly Sensitive and Selective Approach Using Atomic‐Force Microscopy

et al. 2003

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“…The photolithographic technology used to make that array was well discussed in an earlier review 2. Three books on DNA microarrays have been published,3 and the area has been reviewed recently,4,5 and discussed in the popular science press 6…”

Section: Introductionmentioning

confidence: 99%

How to Make a DNA Chip

Pirrung

2002

Angewandte Chemie International Edition

441

117

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Microarrays are one of the hottest areas in biological research today. Microarrays have been mostly applied to nucleic acid analysis, specifically to the assessment of which genes are being expressed and at what level. Early microarrays were prepared by using photolithographic methods, which were more commonly used for integrated circuit (“computer chip”) production. Hence the colloquial term “DNA chip” came into being. The completion of the sequencing of the human genome and that of many other organisms makes the determination of gene function an important next step in understanding the role of DNA in the processes of life. DNA microarrays are an excellent tool to address this question because their numerous probe sites enable the analysis of many genes simultaneously. With good experience in this initial use, many further applications of microarrays are being developed, including genotyping in research and genetic diagnosis in medicine. DNA microarrays have made abundantly clear the power of vast parallelism in biological analysis, which is raising interest in other types of microarrays (small‐molecule, protein). Many applications for DNA microarrays have been developed and clearly many more will emerge through the creativity of the scientists who use them. In early studies, users produced their own microarrays. The apparent power of microarrays has demanded improvements in production methods, and technologies from physical sciences and engineering are now being applied to DNA chips. Many branches of chemistry can contribute to improved methods: from synthetic chemistry (to attach or prepare DNA), to the physical chemistry of surfaces, to analytical chemistry (to assess surface reactions).

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“…16, 255 Die Oligonucleotid‐dirigierte Immobilisierung von DNA‐Protein‐Konjugaten zeichnet sich durch eine hohe Stabilität und Selektivität aus. Ferner sind DNA‐Chips leicht zugänglich 27. 28 Schwierig ist jedoch die selektive Anbindung von Oligonucleotiden an große Proteine.…”

Section: Strategien Zur Proteinimmobilisierungunclassified

“…Proteinarrays werden in der Regel durch Aufbringen von Proteinen mit Kontakt‐ oder Nichtkontakt‐Druckverfahren, die aus der gängigen Hochdurchsatzproduktion von DNA‐Mikroarrays bekannt sind, auf chemisch aktivierte Glasobjektträger hergestellt 27. 28 Für die Erzeugung von Proteinmustern oder nanometergroßen Proteinarrays wurden komplexere Methoden zur Musterbildung entwickelt, deren Herkunft im Bereich der Nanotechnologie und der Materialtechnik liegt 29.…”

Section: Introductionunclassified

Chemische Verfahren zur Herstellung von Proteinbiochips

et al. 2008

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Proteinbiochips sind integrale Bestandteile einer wachsenden Zahl medizinischer und bioanalytischer Anwendungen, weshalb das Interesse an Methoden zur Proteinimmobilisierung für die Herstellung solcher Chips in den letzten Jahren stark gestiegen ist. Dieser Aufsatz gibt einen Überblick über chemische Verfahren zur kovalenten und nichtkovalenten Anbindung von Proteinen auf Oberflächen, wobei ein spezielles Augenmerk auf chemische Selektivität und die Erhaltung der Proteinfunktion bei der Immobilisierung gelegt wird. Ferner werden Strategien zur Herstellung strukturierter Proteinoberflächen umrissen. Abschließend wird ein Ausblick auf mögliche Entwicklungsrichtungen im Bereich der Proteinbiochipforschung und ‐anwendung gegeben.

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DNA-Mikroarrays

Cited by 51 publications

References 90 publications

Label‐Free Detection of DNA Hybridization at the Nanoscale: A Highly Sensitive and Selective Approach Using Atomic‐Force Microscopy

Label‐Free Detection of DNA Hybridization at the Nanoscale: A Highly Sensitive and Selective Approach Using Atomic‐Force Microscopy

How to Make a DNA Chip

Chemische Verfahren zur Herstellung von Proteinbiochips

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