DNA polymorphism of HLA class II genes in pauciarticular juvenile rheumatoid arthritis

Abstract

We investigated the DNA restriction fragment length polymorphism (RFLP) of the major histocompatibility complex (MHC) class II genes: HLA-DRB, -DQA, -DQB, DPA, and -DPB in 54 patients with pauciarticular juvenile rheumatoid arthritis (PJRA) and in healthy Danes. The frequencies of DNA fragments associated with the following HLA class II genes were increased in PJRA when compared to normal controls: DRB1*08 (DRw8) (35.2% vs 10.3%, RR = 4.6, p less than 10(-3), DRB3*01/02/03 (DRw52) (76.3% vs 48.1%, RR 3.5, p less than 10(-3)), DQA1*0401 (41.0% vs 7.4%, RR = 7.9, p less than 10(-3)), DQA1*0501 (55.6% vs 29.7%, RR = 3.0, p less than 10(-2), DQB1*0301 (DQw7) (46.2% vs 17.5%, RR = 4.0; p less than 10(-2)), DPA1*0201 (44.2% vs 7.9%, RR = 8.7, p less than 10(-5)), and DPB1*02 (DPw2) (40.7% vs 7.1%, RR = 8.5, p less than 10(-6)). The frequency of DRB1*11 was not significantly increased. The frequencies of DNA fragments associated with the following HLA class II genes were decreased in PJRA although not statistically significantly so after 'correction' of p values: DRB1*04 (14.8% vs 40.2%, RR = 0.27; p less than 10(-3)), DRB1*07 (0% vs 25.9%, RR = 0.04, p less than 10(-3)), DRB4*0101 (DRw53) (25.9% vs 53.6%, RR = 0.31, p less than 10(-3)), DQA1*0102 (11.6% vs 36.0%, RR = 0.25, p less than 10(-4)), and DQA1*0201 (2.6% vs 34.2%, RR = 0.05, p less than 10(-2)).(ABSTRACT TRUNCATED AT 250 WORDS)

Immunogenetics of multiple sclerosis and optic neuritis: DNA polymorphism of HLA class II genes

“…The enzymes were Bam HI, Bgl II, Eco RI, Eco RV, Hind III, Msp I, Pst I, Rsa I, and Taq I for initial screening for polymorphism. The DNA probes used were: an 800 base pair (bp) Sac I/Hind III fragment of a DRB1 cDNA clone; a 770 bp Apa I fragment of a DQA1 cDNA clone; a 1080 bp Pst I fragment of a DQB1 cDNA clone; a 4.8 kb Hind III fragment of a genomic DPA1 clone; a 640 bp Pst I fragment of a DPB1 cDNA clone (Morling et al 1991 Statistical methods. Fisher's exact text was used for the comparison of frequencies of HLA class II DNA fragments.…”

“…The combined presence of different HLA class II DRB, DQA, DQB, DPA, DPB DNA fragments, including those listed in Table 1, were analyzed as previously described (Morling et al 1991) in both MS and ON. No additive effect (synergism or interaction) of the combined presence of two or more of these genetic markers was demonstrated in MS and ON.…”

“…The data were analyzed for additive effect (synergism or interaction) of the combined presence of two or more of the genetic markers by 2 x 2 comparisons as previously described (Morling et al 1991). No such effects could be demonstrated.…”

Immunogenetic features of juvenile rheumatoid arthritis

“…Серия первых иммуногенетических работ выяви ла повышение HLA В27 у детей с ЮРА, что свиде тельствовало о фундаментальных различиях между ЮРА и РА взрослых, который, как оказалось, ассо циирован с HLA-DR4 [21,22]. Появилась надежда, что изучение особенностей иммуногенетического профиля поможет реклассифицировать хроничес кий артрит у детей.…”

“…Большинство исследователей сообщали о наиболее частых ассоциациях ЮРА с антигенами гистосовместимости HLA DR-5, DR-8, DR-1, при этом Dw-2 и Dw-7 указываются как антигены II класса, встречающиеся достоверно реже у боль ных ЮРА, чем в популяции [21]. Интерпретация данных об ассоциациях антигенов II класса с ЮРА неполная и не окончательная.…”