DNA probes detect genomic diversity in Theileria parva stocks

Conrad, Patricia A.; Iams, Keith P.; Brown, Wendy C.; Sohanpal, Baljinder K.; ole-MoiYoi, Onesmo K.

doi:10.1016/0166-6851(87)90085-5

Cited by 117 publications

(80 citation statements)

References 24 publications

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“…We have used a physical mapping approach because no experimental system exists for genetic studies. The map is being used to study the basis of polymorphism among strains (5,14,15) and to locate markers for detecting and analyzing sexual recombination in this parasite. Of particular interest will be the extent of recombination affecting genes encoding epitopes involved in immune protection.…”

Section: Discussionmentioning

confidence: 99%

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Restriction mapping of the genome of the protozoan parasite Theileria parva.

Morzaria¹,

Young²

1992

Proc. Natl. Acad. Sci. U.S.A.

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We have used a modified linking clone strategy and pulsed-field gel electrophoresis to derive a map of the 29 Sfi I and 4 Not I sites in the 10 million base pawr genome of the protozoan parasite Theienaparva. This was achieved in the absence of classical genetic information. The map reveals four chromosomes. Several genes, including those for parasite antigens, have been located on the map, as has the single locus carrying the major repetitive sequence in this organism. The map forms the basis for a study of sexual recombination in T. parva, which will be of importance in the application of present methods ofimmunization. Aspects ofthe mapping strategy may be useful in the study of other small eukaryotic genomes.

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Section: Discussionmentioning

confidence: 99%

“…Of the 33 Sfi I fragments, the orientation of 31 is known in that at least one end is identified by a linking clone or a telomere. We have located several cloned genes of T. parva, including a major repetitive sequence (14,15) on the map (Fig. 7).…”

mentioning

confidence: 99%

Restriction mapping of the genome of the protozoan parasite Theileria parva.

Morzaria¹,

Young²

1992

Proc. Natl. Acad. Sci. U.S.A.

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“…Do these complex parasites mix at random in the tick and give rise to new parasite types through recombination or are they structured into a few strains that develop clonally? Why, for instance, are cattle parasites so homogeneous in the highveldt of Zimbabwe, while cattle-derived parasites in eastern Africa are generally heterogeneous (Conrad et al 1987;Allsopp & Allsopp 1988)? Are these generally more heterogeneous mixtures because of the closer association with buffalo and or because of more rapid cycling?…”

Section: Epidemiology Of Diseases Caused By Theileria Parva In Eastermentioning

confidence: 99%

Dogmas and misunderstandings in East Coast fever

Dolan¹

1999

Tropical Med Int Health

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SummaryEast Coast fever (ECF) is the most important tick-borne disease in eastern, central and southern Africa and caused an estimated loss of US $186 million in 1989 in the 11 countries where it occurs. It was brought to southern Africa with cattle from Tanzania in 1901 and, over the next 3 years, devastated the cattle that had survived the rinderpest pandemic of the 1890s. Chemical control of ticks using arsenical compounds was introduced in the early 1900s and became the main control measure for both ticks and the diseases they transmit. This method of control has become less reliable over the last 30 years for many reasons, including reduced government spending on livestock and extension, the cost of acaricides, acaricide resistance, poor management of dips and spray races, and poor application of cattle movement control and quarantine. Significant advances in immunization and treatment have been made in the last 30 years, and more robust integrated strategies combining immunization, reduced frequency of chemical control and treatment are being adopted or considered. Throughout its history, ECF has been a source of great anxiety and cost to farmers, and of intense interest to research workers. Many dogmas and misconceptions have become established, some of which still flourish while others took years to demolish. This paper briefly reviews these as well as the history of the disease and explores recent epidemiological findings and their relevance to applying effective control.

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“…Ben Miled, L., Dellagi, K., Bernardi, G., Melrose, T. R., Darghouth, authors found a large degree of polymorphism (Allsopp and M., Bouattour, A., Kinnaird, J., Shiels, B., Tait, A., and Brown, C. Allsopp 1988;Conrad et al 1987;Morzaria et al 1990), G. D. 1994.…”

Section: Figmentioning

confidence: 97%