DNA probes for the detection of Fasciola hepatica in snails

Heussler, Volker; Kaufmann, H.; Strahm, Dora; Liz, Jorge S.; Dobbelaere, Dirk

doi:10.1006/mcpr.1993.1039

Cited by 18 publications

(10 citation statements)

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“…The enzyme electrophoresis in starch gel is a very cheap and simple biochemical method that could be used in a very Abrous et al (1999Abrous et al ( , 2000, Mage et al (2002) c Vignoles et al (2002) d Barthe (1982, 1987) e Van Aken (1982), Van Aken and Brandt (1987) f Shubkin et al (1992), Rognlie et al (1994Rognlie et al ( , 1996 g Heussler et al (1993), Kaplan et al (1995Kaplan et al ( , 1997 h Krämer and Schnieder (1998), Mostafa et al (2003), Velusamy et al (2004), Cucher et al (2006), Caron et al (2007) i Magalhaes et al (2004) j Heussler et al (1993), Schweizer et al (2007) modest laboratory for a preliminary selection of possible intermediate hosts in a particular epidemiological context but the check out can be difficult (enzyme choice, temperature, chemical revelation). The specificity and sensitivity of the technique is poor when compared with DNA/RNA-based techniques and consequently it is not used anymore.…”

Section: Advantages and Disadvantages Of Screening Methodsmentioning

confidence: 99%

“…Real time PCR is difficult to perform particularly with regard to the determination of the experimental conditions of the Abrous et al (1999Abrous et al ( , 2000, Mage et al (2002) c Vignoles et al (2002) d Barthe (1982, 1987) e Van Aken (1982), Van Aken and Brandt (1987) f Shubkin et al (1992), Rognlie et al (1994Rognlie et al ( , 1996 g Heussler et al (1993), Kaplan et al (1995Kaplan et al ( , 1997 h Krämer and Schnieder (1998) Schweizer et al (2007) reaction (design of the TaqMan probe, optimization of E PCR ) and result interpretation. It is an expensive technique because of the TaqMan probe (about 150 Euros), and the price of the fluorescence detection system (about 20,000 Euros).…”

Section: Advantages and Disadvantages Of Screening Methodsmentioning

confidence: 99%

“…The first DNA-based technique that aimed to detect specifically F. hepatica in snails was described in 1993 (Heussler et al 1993). F. hepatica DNA was extracted and digested with Bam HI.…”

Section: Rna-based Blottingmentioning

confidence: 99%

“…and showed over 90% homology with the 18S rRNA genes of trematodes such as Opisthorchis viverrini and various Schistosoma sp. (Heussler et al 1993). The probes were not tested for F. magna nor Paramphistomum sp.…”

Section: Rna-based Blottingmentioning

confidence: 99%

“…Recently, a quantitative real time PCR assay was designed and evaluated in naturally infected G. truncatula (Schweizer et al 2007). Primers and probe were designed to amplify an 86-pb-long target of a repetitive 449-bp-long genomic DNA fragment cloned during a previous study (clone Fhr-II, Genbank accession number, S67037) (Heussler et al 1993). The sensitivity of the detection of F. hepatica with this technique was not evaluated (Schweizer et al 2007).…”

Section: Real Time Pcrmentioning

confidence: 99%

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The detection and quantification of a digenean infection in the snail host with special emphasis on Fasciola sp.

2008

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In this review, ten methods used to study digenean infections in their intermediate hosts were compared to determine which one should be used either in the field or in the lab to establish the prevalence and intensity of infections in snails. Snail crushing and snail dissection allow quick establishing of prevalence in natural or experimental infections, whereas histology is considered as the most accurate approach to assess the intensity of infection. The follow-up of cercarial shedding only gave an idea on cercarial production. Among recently developed techniques, polymerase chain reaction (PCR) brings the most accurate information and shows high sensitivity and specificity levels when compared to blotting techniques. The easiness and relatively low cost of the basic PCR protocol make it interesting to investigate the epidemiology of the liver fluke in a lab with limited financial resources. Nevertheless, if this technique allows a relatively good estimation of the prevalence, information concerning the intensity of infection is best obtained through real time PCR. However, at the time being this technique is too expensive to be used routinely in the field. The choice between classical or new techniques is usually based on a compromise, as each technique has its advantages and drawbacks.

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Section: Advantages and Disadvantages Of Screening Methodsmentioning

confidence: 99%

Section: Advantages and Disadvantages Of Screening Methodsmentioning

confidence: 99%

“…The first DNA-based technique that aimed to detect specifically F. hepatica in snails was described in 1993 (Heussler et al 1993). F. hepatica DNA was extracted and digested with Bam HI.…”

Section: Rna-based Blottingmentioning

confidence: 99%

Section: Rna-based Blottingmentioning

confidence: 99%

Section: Real Time Pcrmentioning

confidence: 99%

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The detection and quantification of a digenean infection in the snail host with special emphasis on Fasciola sp.

2008

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Plant-Borne Trematode Zoonoses: Fascioliasis and Fasciolopsiasis

Mas‐Coma

Bargues

Valero

2007

World Class Parasites

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A DNA probe for the detection of Dicrocoelium dendriticum in ants of Formica spp. and Lasius spp.

Heussler

Kaufmann

Glaser³

et al. 1998

Parasitol Res

Self Cite

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Repetitive DNA sequences present in the genome of Dicrocoelium dendriticum were identified by hybridization of genomic DNA that had been digested with different restriction enzymes with 32P-labeled genomic D. dendriticum DNA. DNA fragments containing repetitive sequences were isolated from PstI-digested D. dendriticum DNA and were subcloned into a plasmid vector. Plasmids containing repetitive sequences were identified by colony hybridization. One of these plasmids, designated Ddr-IV, was isolated and used as a probe in further studies. Ddr-IV is specific for D. dendriticum since it does not hybridize to DNA isolated from other trematodes. In addition, Ddr-IV was capable of detecting D. dendriticum metacercariae in ants (Formica cunicularia, F. rufibarbis, and Lasius sp.), which act as second intermediate hosts in the parasite's life cycle. Since metacercariae constitute the infectious stage of the parasite for grazing animals, Ddr-IV will provide a useful tool for epidemiology studies of dicrocoeliosis.

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DNA probes for the detection of Fasciola hepatica in snails

Cited by 18 publications

References 0 publications

The detection and quantification of a digenean infection in the snail host with special emphasis on Fasciola sp.

The detection and quantification of a digenean infection in the snail host with special emphasis on Fasciola sp.

Plant-Borne Trematode Zoonoses: Fascioliasis and Fasciolopsiasis

A DNA probe for the detection of Dicrocoelium dendriticum in ants of Formica spp. and Lasius spp.

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