DNA Profiles from Fingerprint Lifts—Enhancing the Evidential Value of Fingermarks Through Successful DNA Typing

Subhani, Zuhaib; Daniel, Barbara; Frascione, Nunzianda

doi:10.1111/1556-4029.13830

Cited by 20 publications

(8 citation statements)

References 28 publications

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“…Nowadays, among several LFPs enhancement techniques, depending on the greater surface area of deposition and fluorescence abilities, the role of nanoscience and nanotechnology become an inevitable enhancing agent. In addition to the enhancement, it has also been used to explore genetic profiles, sex, and illicit drug used by perpetrators having little DNA quantities during the deposition of FPs on some surface [2,3]. The deposited FPs at the crime scene is categorized into three parts: (i) the visible (positive/negative), (ii) indented (molded/plastic), and (iii) the latent/invisible prints.…”

Section: Introductionmentioning

confidence: 99%

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Latent Fingerprint Enhancement Techniques: A Review

et al. 2020

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Fingerprint (FP) is a global mark used for personal identification. This study reviews recent latent fingerprint (LFP) enhancement techniques including metal oxides, multi-metal deposition (MMD-I/II/SMD/Au-ASP), optical, chemical, physical, and physicochemical. Furthermore, analytical techniques involved in identification, evaluation, and determination of pieces of evidence, and perpetrators including the SEM, TEM, UV-Vis, IR/NIR, SERS, SKP, DLS, MALDI-MSI, and TD analysis were also discussed. Among numerous LFP enhancement techniques, the application of chemical methods in combination with optical techniques has a greater place to recover FPs with sufficient quality. However, instead of using such a complex and costly enhancing agent, nowadays nanotechnology is using specific techniques used for visualizing, inspecting, gathering, and analyzing trace evidence at the scene of a crime. To indicate using simple metal oxides such as ZnO that have superior fluorescent properties and also that consider both the surface and cost of the materials, enhancement of the LFP is functioning.

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Section: Introductionmentioning

confidence: 99%

“…Detection of fingermarks by colloidal gold (MMD/SMD)-beyond the pH 3 limit. Forensic science international, 219(1)(2)(3),39-49. [11] Choi, M. J., McDonagh, A. M.,Maynard, P., nanoparticles and nano-structured particles in fingermark detection.…”

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confidence: 99%

Latent Fingerprint Enhancement Techniques: A Review

et al. 2020

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“…DNA quantification was performed using the Qubit ds-DNA Assay Kit (Life Technologies, Carlsbad, CA) along with the Qubit Fluorometer 3.0, according to the manufacturer's protocol and previously published protocol [10]. Quantified DNA was measured in units of concentration by ng/mL.…”

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confidence: 99%

“…It seems swabbing should be the preferred technique to optimize the recovery of DNA, particularly touch DNA. The nondestructive nature of swabbing also allows for potential recovery of other type of evidence, such as fingerprints, as described on previous studies [10,11]. The nature of these samples must also be considered, contact lenses, eyeglasses, and earphones might be worn for the entire day by an individual, therefore, high rates of DNA recovery in comparison to other items are expected [2,12].…”

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confidence: 99%

From your eyes only: Efficiency of nuclear and mitochondrial DNA isolation from contact lenses at crime scenes

et al. 2020

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Recovering appropriate evidence from crime scenes to identify the perpetrator, or in certain cases, the victim, is one of the main goals of forensic investigation. The current practice includes collection of objects from which "trace" or "touch" DNA may be retrieved. Contact lenses, worn by the perpetrator/victim, represent a potentially good source of DNA. This is based on their exposure to touch DNA and the release and regeneration of epithelial cells from the eye. This study takes these premises into account with the objective of analyzing the efficiency of human nuclear and mitochondrial DNA isolation from contact lenses. DNA was collected using a non-destructive technique (double-swab) and subsequently isolated using a silica-based methodology. Although DNA quantification was variable among subjects and contact lenses, it was sufficient to amplify some STRs and successfully sequence mtDNA. The findings from this research provide a proof-of-concept that it is possible to recover, isolate, amplify human nuclear DNA, and characterize mitochondrial DNA from contact lenses left at crime scenes. These results show the potential importance of recovering unusual items from crime scenes as a potential source of DNA to identify the victim and/or suspect.

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“…The effect of fingerprint powders on subsequent DNA analysis has been well documented over the last 15 years . The majority of studies have looked at the impact of fingerprint powders on subsequent DNA analysis, with a focus on: the effect of various substrates ; the quality of fingerprints ; the effect of time ; the effect of materials routinely used in forensic investigations ; and various fingerprint treatments . Another study looked at investigating the ability of recovering DNA from a gel lifter instead of sampling the fingerprint in situ .…”

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confidence: 99%

Evaluation of DNA Extraction Methods for Processing Fingerprint Powder‐Coated Forensic Evidence

Cornwell

Tay

Allan

et al. 2019

Journal of Forensic Sciences

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In unison, fingerprinting and DNA analysis have played a pivotal role in forensic investigations. Fingerprint powders that are available on the market can come in a range of colors and with specific properties. This study evaluated the efficiency of DNA extraction from samples coated with 3 brands of fingerprint powders: Lightning, Sirchie, and SupraNano, covering a range of colors and properties. A total of 23 fingerprint powders were tested using the Chelex, Promega DNA IQ TM , and Applied Biosystems TM PrepFiler TM DNA extraction protocols. The DNA IQ TM and PrepFiler TM methods extracted higher yields of DNA in comparison to Chelex, which also accounted for better quality of PowerPlexâ 21 DNA profiles recovered. There were no signs of degradation or inhibition in the quantification data, indicating that samples returning low DNA yield was due to interference during DNA extraction and not PCR inhibition. DNA profiles were recovered from the majority of fingerprint powders with only a single powder, Sirchie Magnetic Silver, failing to produce a profile using any of the methods tested. A link was observed between the DNA extraction chemistry, fingerprint powder property, that is, nonmagnetic, magnetic and aqueous, and the brand of fingerprint powder. Overall, the DNA IQ TM method was favorable for nonmagnetic fingerprint powders, while magnetic fingerprint powders produced more DNA profiles when extracted with the PrepFiler TM chemistry. This study highlights the importance of screening DNA extraction chemistries for the type of fingerprint powder used, as there is not a single DNA extraction method that suits all fingerprint powder brands and properties.

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DNA Profiles from Fingerprint Lifts—Enhancing the Evidential Value of Fingermarks Through Successful DNA Typing

Cited by 20 publications

References 28 publications

Latent Fingerprint Enhancement Techniques: A Review

Latent Fingerprint Enhancement Techniques: A Review

From your eyes only: Efficiency of nuclear and mitochondrial DNA isolation from contact lenses at crime scenes

Evaluation of DNA Extraction Methods for Processing Fingerprint Powder‐Coated Forensic Evidence

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