DNA protection activity of a hydroethanol extract and six polyphenol monomers fromMorus alba L. (mulberry) twig

Abstract

Morus alba L. (mulberry) is one of the most important medicine and food crops in China and other developing countries in Asia due to its large biomass (twigs) and useful by-products. Additionally, its twigs are an excellent green resource for bioactive compounds that are resistant to ultraviolet (UV) radiation and that can whiten skin and scavenge free radicals, and are of potential interest to the cosmetics and health food industries. In this study, we evaluated the ability of Morus alba L. twig hydroethanol extract and isolated compounds to protect DNA against UV damage. Agarose gel electrophoresis showed that a hydroethanol extract and six different polyphenol monomers (oxyresveratrol, resveratrol, mulberroside A, rutin, quercetin, and morin) present in the extract protected pUC19 plasmid DNA from UV damage. The optimal concentrations of polyphenols and Morus alba L. twig extract for DNA protection were 0.500 mg/mL and 1.00 mg/mL, respectively. These compounds may have potential as skin-care products to prevent UV-induced damage to the skin. Additionally, activity contribution degree assessment revealed other Morus alba L. substances possessing significant DNA protection activity that are worthy of further investigation. ARTICLE HISTORY

Quantitative Comparison of the Marker Compounds in Different Medicinal Parts of Morus alba L. Using High-Performance Liquid Chromatography-Diode Array Detector with Chemometric Analysis

“…The root is considered a substantial site for the biosynthesis of mulberroside A via glycosylation of oxyresveratrol (hydroxy-resveratrol), which is found as a precursor in the root of M. alba [35]. Oxyresveratrol is known as one of the key compounds in M. alba twigs, following its monoor di-glucosylated moieties [36][37][38], which supports the non-statistically significant higher amount in the twigs than in the root barks in this study.…”

Mulberroside A repairs high fructose diet‐induced damage of intestinal epithelial and blood–brain barriers in mice: A potential for preventing hippocampal neuroinflammatory injury

“…Mulberroside A possesses pharmacological functions, such as antioxidant, anti-inflammatory, and anti-tyrosinase activities (Kim et al, 2010;Lu et al, 2017;Zhang & Shi, 2010). The results presented in the current study showed that mulberroside A reshaped gut dysbiosis, increased fecal and circulating SCFAs, reactivated colonic NLRP6 inflammasome signaling, and inhibited oxidative stress, resulting in its protection against IEB damage to reduce serum endotoxin (toxemia) in HFrD-fed mice.…”

“…Our previous study showed that dietary HFrD‐driven gut dysbiosis, SCFAs metabolic disorder and NLRP6 inflammation signaling disruption caused IEB damage, which were possibly associated with the occurrence of hippocampal neuroinflammation (Li et al., 2019). Mulberroside A possesses pharmacological functions, such as antioxidant, anti‐inflammatory, and anti‐tyrosinase activities (Kim et al., 2010; Lu et al., 2017; Zhang & Shi, 2010). The results presented in the current study showed that mulberroside A reshaped gut dysbiosis, increased fecal and circulating SCFAs, reactivated colonic NLRP6 inflammasome signaling, and inhibited oxidative stress, resulting in its protection against IEB damage to reduce serum endotoxin (toxemia) in HFrD‐fed mice.…”

UPLC-MS/MS Profiling, Antioxidant, α-Glucosidase Inhibitory, Cholinesterase Inhibitory, and Cardiovascular Protection Potentials of Jialing 20 (Morus multicaulis Perr.) Mulberry Branch Extract

“…Mulberry branches, also known as Ramulus mori (RM), are included in the Chinese Pharmacopoeia as a herbal beverage, and mulberry branch extract (MBE) has been approved as a food source by the Chinese and Korean governments [3,4], indicating that MBE is safe for consumption. Mulberry branches are rich in active components such as flavonoids [5], alkaloids [1], and polysaccharides [6], which have various activities such as antioxidation [7] and hypoglycemic effects [8]. However, the chemical composition and biological activities of MBE of different varieties of mulberry trees vary greatly [7,9,10].…”