DNA purification using a novel γ-Fe2O3/PEDOT hybrid nanocomposite

Immobilization of Multivalent Titanium Cations on Magnetic Composite Microspheres for Highly Efficient DNA Extraction and Amplification

“…To better understand the binding nature of the adsorption process, we tested the variation of the adsorption efficiency at various DNA concentrations and fitted the experimental data to Langmuir and Freundlich isothermal adsorption models (Figure ). , An isotherm obeys the Langmuir model when the adsorption occurs with formation of a monolayer on the adsorbed surface, and the relationship is as following C normale Q normale = 1 b q m + C normale q normalm where C e (mg/L) is the concentration of DNA in the solution at equilibrium state, Q e (mg/g) is the amount of DNA per unit mass of MM@PGMA–PA-Ti 4+ , b is the Langmuir constant, and q m (mg/g) is the maximum DNA adsorption capacity.…”

“…To better understand the binding nature of the adsorption process, we tested the variation of the adsorption efficiency at various DNA concentrations and fitted the experimental data to Langmuir and Freundlich isothermal adsorption models (Figure 5). 19,47 An isotherm obeys the Langmuir model when the adsorption occurs with formation of a monolayer on the adsorbed surface, and the relationship is as following…”

“…In order to improve DNA extraction efficiency, recent research has an increased interest in using cationic-functionalized magnetic composite microspheres. ,,– Efficient DNA loading can be achieved by the electrostatic interaction of positively charged magnetic beads with the phosphate groups of DNA. However, despite their potential benefits, there are still some limitations for practical applications, such as excessive elution time and harsh desorption conditions. , , , For example, the maghemite nanoparticles coated with poly­(3,4-ethylenedioxythiophene) chains (γ-NP/PEDOT) was developed by Da Silva et al, and the electrostatic interaction can be adjusted by varying pH for efficient DNA adsorption, but the use of γ-NP/PEDOT raised concerns about the total time required for complete DNA retrieval and the quality of the purified DNA.…”

“…In order to improve DNA extraction efficiency, recent research has an increased interest in using cationic-functionalized magnetic composite microspheres. ,,– Efficient DNA loading can be achieved by the electrostatic interaction of positively charged magnetic beads with the phosphate groups of DNA. However, despite their potential benefits, there are still some limitations for practical applications, such as excessive elution time and harsh desorption conditions. , , , For example, the maghemite nanoparticles coated with poly­(3,4-ethylenedioxythiophene) chains (γ-NP/PEDOT) was developed by Da Silva et al, and the electrostatic interaction can be adjusted by varying pH for efficient DNA adsorption, but the use of γ-NP/PEDOT raised concerns about the total time required for complete DNA retrieval and the quality of the purified DNA. Meanwhile, Maciel et al synthesized magnetic composite nanoparticles with chitosan and polyaniline shell to extract and purify DNA from whole human blood.…”

Advances in the use of nanomaterials for nucleic acid detection in point-of-care testing devices: A review

“…The result demonstrated the extraction efficiency of 1 CFU/ml was 10 times better than that of the commercial kit, and that the process had good stability and accuracy. A novel g-Fe 2 O 3 /PEDOT hybrid nanocomposite was synthesized by da Silva et al (2021 ). Here modified nanomagnetic beads were used to extract the DNA from blood and bacteria, and the yields after verification were 6.4 mg and 7.3 mg, respectively, and the captured DNA could be directly used for downstream experiments, such as PCR amplification, without being detached from the nanocomposite.…”