DNA Robots for CRISPR/Cas12a Activity Management and Universal Platforms for Biosensing
Xinyi Xia,
Qiutong Chen,
Tongshan Zuo
et al.
Abstract:The CRISPR/Cas12a system is a revolutionary genome editing technique that is widely employed in biosensing and molecular diagnostics. However, there are few reports on precisely managing the trans-cleavage activity of Cas12a by simple modification since the traditional methods to manage Cas12a often require difficult and rigorous regulation of core components. Hence, we developed a novel CRISPR/Cas12a regulatory mechanism, named DNA Robots for Enzyme Activity Management (DREAM), by introducing two simple DNA r… Show more
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