DNA sperm damage correlates with nuclear ultrastructural sperm defects in teratozoospermic men

Skowronek, Fernanda; Casanova, Gabriela; Alciaturi, Jimena; Capurro, Ana; Cantu, Lidia; Montes, José María de las Heras; Sapiro, Rossana

doi:10.1111/j.1439-0272.2010.01106.x

Cited by 33 publications

(28 citation statements)

References 29 publications

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“…On the other hand, the ultrastructural morphology of sperm of O. niloticus collected from LM displays some alternations including nuclear distortion changes in head morphology, incomplete chromatin condensation, and shortage of bilateral fins of the flagellum, wavy and ruptured plasma membrane. As well as, the presence of some vacuoles and the relative decreases in the density of chromatin condensation might be associated with chromosomal abnormalities, which is associated with decrease fertility potential [46,47]. Moreover, LM fish's spermatozoa revealed the presence of few numbers and large sized mitochondria in front of the proximal centriole toward the lateral aspects of the nucleus.…”

Section: Discussionmentioning

confidence: 99%

Impact of Environmental Contaminants on the Testes of Oreochromis niloticus with Special Reference to Ultrastructure of Spermatozoa in Lake Manzala (Egypt)

Shalaby¹,

Migeed²

2012

J Environ Anal Toxicol

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IntroductionThe importance of Nile tilapia, Oreochromis niloticus in aqua cultures is due to its spreading in most countries of the world [1]. It breads in captivity and wide variety of water conditions [2]. Lake Manzalah (LM) is one of the most important lakes in Egypt that provides more than 70% of the total fishery of the country [3] and considered as a highly polluted with toxic heavy metals and pesticides due to progressive increase of industrial and agricultural drainage, as well as sewage out fall from the surrounding governorates [4]. The mean values of some heavy metals (Cd, Pb, Fe, Cr and Mn) recorded in LM were higher than the world permissible levels [5] and according to the Egyptian laws [6]. Such heavy metals are not lethal to fish, but concentrated in their flesh and create a hazard to the consumer [7,8]. Cd, Pb, Zn, and Cr are considered the main causes of pollution in aquatic ecosystem [9]. The serious effect of these metals is derived from their persistence, toxicity and bioaccumulation, and consequently exert dangerous problem to man [10]. Experimental studies on heavy metals proved that they could impair the respiratory functions of the gills; consequently death in fish is probably caused by tissue hypoxia [11]. Metals affect not only the fish morphology, but also all biological activities [12]. Among the heavy metals; Cd and Pb are the serious heavy metals that produce histopathological alterations including liver damage [13], respiratory dysfunctions [14], testicular and ovarian alterations [15][16][17]. These pathological alterations were primarily attributed to damage of cell membranes which allows higher uptake of Cd and Pb and thus the injury extends to more critical targets [18]. Many studies focused on bioaccumulation of heavy metals in fish and biological studies. Thus we aimed in this work to study the testicular histopathological alterations of Oreochromis niloticus using light microscope and the mature sperm using TEM. Materials and Methods Samples collectionSamples of water and 48 specimen of Nile tilapia, Oreochromis niloticus about 14-20 cm in length and 120-200 g in weight were collected from two areas; Demitta branch of RN at Mansoura city as a control site and LM as a polluted area during the spawning season from March-June and transported to the laboratory in tanks provided with oxygen pump. The fish were weighed, dissected and the testes were Abstract Background: Pollution of the aquatic environment by inorganic and organic chemicals is a major factor posing serious threat to the survival of aquatic organisms including fish. Lake Manzala (LM) is one of the Egypt's northern Delta Lakes situated on the Mediterranean Coast of the Delta. It is affected by drainage water polluted by different heavy metals that their concentrations exceed the maximum world permissible levels.

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Section: Discussionmentioning

confidence: 99%

Impact of Environmental Contaminants on the Testes of Oreochromis niloticus with Special Reference to Ultrastructure of Spermatozoa in Lake Manzala (Egypt)

Shalaby¹,

Migeed²

2012

J Environ Anal Toxicol

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“…The mechanisms proposed to resolve the etiology of DNA damage include apoptosis, oxidative stress, and abnormal chromatin packaging [Ménézo et al 2010;Miller et al 2010]. Many studies have indicated that there is a positive relationship between sperm DNA fragmentation and large nuclear vacuoles in the sperm nuclear area [Oliveira et al 2010a;Skowronek et al 2012;Sakkas and Alvarez 2010]. Furthermore, several studies reported that large vacuoles were linked with chromatin condensation failure [Gopalkrishnan et al 2000;Perdrix et al 2011;Boitrelle et al 2011;Franco et al 2012;Cassuto et al 2012].…”

Section: Discussionmentioning

confidence: 99%

Sperm with large nuclear vacuoles and semen quality in the evaluation of male infertility

Komiya

Watanabe

Kawauchi

et al. 2012

Systems Biology in Reproductive Medicine

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This study compared the sperm nuclear vacuoles and semen quality in the evaluation of male infertility. One hundred and forty-two semen samples were obtained from patients who visited the Male Infertility Clinic at Toyama University Hospital. Semen samples were evaluated by conventional semen analyses and the Sperm Motility Analysis System (SMAS). In addition, spermatozoa were analyzed at 3,700-6,150x magnification on an inverted microscope equipped with DIC/Nomarski differential interference contrast optics. A large nuclear vacuole (LNV) was defined as one or more vacuoles with the maximum diameter showing > 50% width of the sperm head. The percentage of spermatozoa with LNV (% LNV) was calculated for each sample. Correlations between the % LNV and parameters in SMAS and conventional semen analyses were analyzed. Processed motile spermatozoa from each sample were evaluated. The mean age of patients was 35 years old. Semen volume was 2.9 ± 1.6mL (0.1-11.0; mean ± standard deviation, minimum-maximum), sperm count was 39.3 ± 54.9 (x10 6 /mL, 0.01-262.0), sperm motility was 25.1 ± 17.8% (0-76.0), and normal sperm morphology was 10.3 ± 10.1% (0-49.0). After motile spermatozoa selection, we could evaluate % LNV in 125 ejaculates (88.0%) and at least one spermatozoon with LNV was observed in 118 ejaculates (94.4%). The percentage of spermatozoa with LNV was 28.0 ± 22.4% (0-100) and % LNV increased significantly when semen quality decreased. The correlation between the % LNV and the semen parameters was weak to moderate; correlation coefficients were -0.3577 in sperm count ( p < 0.0001), -0.2368 in sperm motility ( p = 0.0084), -0.2769 in motile sperm count ( p = 0.019), -0.2419 in total motile sperm count ( p = 0.0070), and -0.1676 in normal sperm morphology ( p = 0.0639). The % LNV did not show a significant correlation with the SMAS parameters except for weak correlation to beat/cross frequency (r = -0.2414, p = 0.0071). The percentage of spermatozoa with LNV did not have a strong correlation with parameters in conventional semen analysis and SMAS in the patients with male infertility; however, a certain level of negative influence of LNV to sperm quality cannot be excluded.

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“…The ultrastructure of sperm head, neck and tail can be analyzed and quantified by scanning or transmission electron microscopy (Moretti et al 2007, Collodel et al 2009a,b, Skowronek et al 2010, Visco et al 2010, Skowronek et al 2012, Collodel et al 2013, Gatimel et al 2013.…”

Section: Evaluation Of Sperm Morphologymentioning

confidence: 99%

Evaluation of morphological criteria of sperm quality before in vitro fertilization and intracytoplasmic sperm injection

Lasienë

Gedrimas

Vitkus

et al. 2013

Polish Journal of Veterinary Sciences

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The quality of sperm has a direct influence on the fertilization and developmental competence of embryos. In the literature we did not find defined criteria for evaluation of normal sperm parameters in various species of domestic mammals. Therefore we attempted to review evaluation of criteria of morphologically normal human sperm and their abnormalities. All sperm cells observed in the stained sample are classified as normal or abnormal. Any abnormalities in morphology of sperm have a negative effect on the outcome in in vitro fertilization and intracytoplasmic sperm injection. Abnormal sperm are categorized into subgroups according to the observed defects (concerning the head and/or midpiece and/or tail). Most morphologically abnormal sperm have multiple defects. This article can be considered as guideline for the manual of sperm quality evaluation in different species of domestic mammals.

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DNA sperm damage correlates with nuclear ultrastructural sperm defects in teratozoospermic men

Cited by 33 publications

References 29 publications

Impact of Environmental Contaminants on the Testes of Oreochromis niloticus with Special Reference to Ultrastructure of Spermatozoa in Lake Manzala (Egypt)

Impact of Environmental Contaminants on the Testes of Oreochromis niloticus with Special Reference to Ultrastructure of Spermatozoa in Lake Manzala (Egypt)

Sperm with large nuclear vacuoles and semen quality in the evaluation of male infertility

Evaluation of morphological criteria of sperm quality before in vitro fertilization and intracytoplasmic sperm injection

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