DNA strand breaks and chromosomal aberrations induced by H2O2 and 60Co γ-radiation

Rueff, José; Brás, Aldina; Cristóvão, L.; Mexia, João T.; Costa, M Sá da; Pires, Virgínia

doi:10.1016/0027-5107(93)90070-v

Cited by 58 publications

(25 citation statements)

References 21 publications

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“…In the Comet assay, which was more sensitive than the yeast test, all the disinfectants were able to induce genotoxicity: the lowest effective dose for NaClO and PAA was 0.5 mg/l, whereas for ClO 2 , it was 0.2 mg/l. These findings confirm the mutagenicity of NaClO found by Wlodkowki and Rosenkranz [1975] using the Ames test, and the mutagenicity of H 2 O 2 , present at equilibrium in PAA solutions, found by Rueff et al [1993]. The genotoxicity of ClO 2 , first reported by Buschini et al [2004b], was also confirmed in the present study.…”

Section: Discussionsupporting

confidence: 93%

“…Therefore, health hazards could result from chronic exposure to these free disinfectant residues. Chlorine, the most commonly used disinfectant, has in fact been found to be carcinogenic [Soffritti et al, 1997], and sodium hypochlorite (NaClO) [Wlodkowski et al, 1975;Ishidate et al, 1988;Gauthier et al, 1989] and hydrogen peroxide (H 2 O 2 ) [Ishidate, 1988;Muller and Janz, 1993;Rueff et al, 1993] have displayed mutagenic activity in both in vitro and in vivo short-term tests.…”

Section: Introductionmentioning

confidence: 99%

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Genotoxicity of drinking water disinfectants in plant bioassays

Monarca

Feretti

Zani

et al. 2005

Environ and Mol Mutagen

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The genotoxicity of two widely used drinking water disinfectants, sodium hypochlorite (NaClO) and chlorine dioxide (ClO2), and a new disinfectant, peracetic acid (PAA, CH3‐CO‐COOH), was evaluated in three short‐term plant tests: (1) induction of anaphase chromosome aberrations in the root cells of Allium cepa, (2) micronucleus induction in the root cells of Vicia faba, and (3) micronucleus induction in Tradescantia pollen cells. The study was carried out in the laboratory by directly exposing the plants to several concentrations of the disinfectants in redistilled water at unadjusted (acid) and adjusted (neutral) pHs. Both 0.1 and 0.2 mg/l NaClO induced chromosome aberrations in the Allium cepa test at acid pH, but concentrations up to 0.5 mg/l of all the disinfectants were negative at neutral pH. Concentrations ranging from 0.1 to 0.5 mg/l NaClO, ClO2, and PAA induced micronuclei in Vicia faba at acid pH, while 1–2 mg/l NaClO and ClO2 and 0.5–2 mg/l PAA gave positive responses at neutral pH. Most of concentrations of ClO2 produced positive responses in the Tradescantia micronucleus test. In general, the highest levels of genotoxicity were observed under acid conditions; at acid pH, significant effects were induced by low concentrations of ClO2 and PAA. Since the test concentrations of disinfectants are typical of those encountered in the biocidal treatment of tap water and similar concentrations are consumed daily by a large number of people, the genotoxicity of these compounds may constitute a significant public health concern. Environ. Mol. Mutagen., 2005. © 2005 Wiley‐Liss, Inc.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Genotoxicity of drinking water disinfectants in plant bioassays

Monarca

Feretti

Zani

et al. 2005

Environ and Mol Mutagen

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“…Absorbance of each well was quantified at 550 nm using 620 nm as reference wavelength on a scanning multiwell spectrophotometer (automated plate reader). Genotoxicity was assessed by the short-term in vitro chromosomal aberration assay according to Rueff et al (1993). Briefly, cell cultures of peripheral blood lymphocytes from healthy donors were set up, and lymphoproliferation was induced with phytohemagglutinnin (2% v/v, 24 h incubation).…”

Section: Toxicity Studiesmentioning

confidence: 99%

Non-toxic Salvia sclareoides Brot. extracts as a source of functional food ingredients: Phenolic profile, antioxidant activity and prion binding properties

et al. 2012

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a b s t r a c tSalvia sclareoides is an aromatic herb native to Portugal, of which phenolic content (Folin-Ciocalteau method), chemical profile (HPLC/DAD), antioxidant activity (DPPH, b-carotene/linoleic acid assays), acute toxicity (MTT method, adapted for non-adherent cells), genotoxicity (short-term chromosomal aberration assay) and prion binding properties were evaluated in the acetone, water, ethanol, methanol and n-butanol extracts. The latter presented the highest phenolic content and antioxidant activity (DPPH assay), and was the single one with the flavonoids (+)-catechin, kaempferol O-glucoside and quercetin. Vanillic acid was the major component of all extracts but gallic, gentisic, caffeic, syringic, coumaric and ferulic acids were also found in some extracts. Only the n-butanol extract had components binding to the cellular form of human prion protein detected by NMR which showed specificity for two regions of the folded domain and for the unstructured N-terminal region. Extracts were not cytotoxic nor genotoxic, reinforcing the potential of S. sclareoides for nutraceutical purposes.

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“…Hydrogen peroxide was chosen as the model ROS due to its ease of administration and as it is thought to represent a diffusible form of ROS. This dose was chosen as it was subtoxic and has previously been shown to cause DNA/chromosomal damage (Rueff et al, 1993;Perwez Hussain et al, 1994;Duthie et al, 1997;Fenech et al, 1999;Umegaki and Fenech, 2000;Jenkins et al, 2001). Initially, the micronucleus assay was performed to determine if chromosome damage was induced following standard protocols (Fenech and Morley, 1985;Fenech et al, 1999).…”

Section: In Vitro Ros Studymentioning

confidence: 99%

Fluorescence in situ hybridisation analysis of chromosomal aberrations in gastric tissue: the potential involvement of Helicobacter pylori

et al. 2005

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In this series of experiments, a novel protocol was developed whereby gastric cells were collected using endoscopic cytology brush techniques, and prepared, such that interphase fluorescence in situ hybridization (FISH) could be performed. In total, 80 distinct histological samples from 37 patients were studied using four chromosome probes (over 32 000 cells analysed). Studies have previously identified abnormalities of these four chromosomes in upper GI tumours. Using premalignant tissues, we aimed to determine how early in Correa's pathway to gastric cancer these chromosome abnormalities occurred. Aneuploidy of chromosomes 4, 8, 20 and 17(p53) was detected in histologically normal gastric mucosa, as well as in gastritis, intestinal metaplasia, dysplasia and cancer samples. The levels of aneuploidy increased as disease severity increased. Amplification of chromosome 4 and chromosome 20, and deletion of chromosome 17(p53) were the more common findings. Hence, a role for these abnormalities may exist in the initiation of, and the progression to, gastric cancer. Helicobactor pylori infection was determined in premalignant tissue using histological analysis and PCR technology. Detection rates were comparable. PCR was used to subtype H. pylori for CagA status. The amplification of chromosome 4 in gastric tissue was significantly more prevalent in H. pylori-positive patients (n ¼ 7) compared to H. pylori-negative patients (n ¼ 11), possibly reflecting a role for chromosome 4 amplification in H. pylori-induced gastric cancer. The more virulent CagA strain of H. pylori was associated with increased disease pathology and chromosomal abnormalities, although numbers were small (CagA þ n ¼ 3, CagAÀ n ¼ 4). Finally, in vitro work demonstrated that the aneuploidy induced in a human cell line after exposure to the reactive oxygen species (ROS) hydrogen peroxide was similar to that already shown in the gastric cancer pathway, and may further strengthen the hypothesis that H. pylori causes gastric cancer progression via an ROS-mediated mechanism.

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DNA strand breaks and chromosomal aberrations induced by H2O2 and 60Co γ-radiation

Cited by 58 publications

References 21 publications

Genotoxicity of drinking water disinfectants in plant bioassays

Genotoxicity of drinking water disinfectants in plant bioassays

Non-toxic Salvia sclareoides Brot. extracts as a source of functional food ingredients: Phenolic profile, antioxidant activity and prion binding properties

Fluorescence in situ hybridisation analysis of chromosomal aberrations in gastric tissue: the potential involvement of Helicobacter pylori

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