DNA supercoiling during ATP-dependent DNA translocation by the type I restriction enzyme EcoAI

Janscak, Pavel; Bickle, Thomas A.

doi:10.1006/jmbi.1999.3414

Cited by 40 publications

(33 citation statements)

References 50 publications

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“…Given the nature of the activity of the enzyme, it is not sur-prising that the so-called R subunit of all type I restriction endonucleases contains the seven SF2 helicase motifs (10,15). This is consistent with the notion that the SF2 core acts as a ds translocase without opening the duplex (19). In this regard, it is noteworthy that experiments with psoralen-cross-linked DNA (13) showed that, at low levels, the interstrand crosslinks did not impede translocation, suggesting that the enzyme tracked along the DNA backbone without opening the strands.…”

Section: Related Systemsmentioning

confidence: 59%

Modularity and Specialization in Superfamily 1 and 2 Helicases

Singleton¹,

Wigley²

2002

J Bacteriol

198

167

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Section: Related Systemsmentioning

confidence: 59%

Modularity and Specialization in Superfamily 1 and 2 Helicases

Singleton¹,

Wigley²

2002

J Bacteriol

198

167

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“…Here, remodelers may remain in a fixed position on the nucleosome and rotate the DNA duplex. However, DNA helix rotation may also occur as a consequence of DNA translocation (Janscak and Bickle 2000). If the remodeler translocates by tracking along one strand and remains in a fixed position on the histone octamer, the DNA must undergo one full rotation for every 10.6 bp of translocation.…”

Section: Remodeling Is Less Efficient On Nicked Nucleosomesmentioning

confidence: 99%

Chromatin remodeling by RSC involves ATP-dependent DNA translocation

2002

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establish that RSC, as well as its isolated ATPase subunit Sth1, are DNA translocases. RSC/Sth1 ATPase activity is stimulated by single-stranded DNA, suggesting that Sth1 tracks along one strand of the DNA duplex. Each RSC complex appears to contain a single molecule of Sth1, and isolated Sth1 is capable of nucleosome remodeling. We propose that the remodeling enzyme remains in a fixed position on the octamer and translocates a segment of DNA (with accompanying DNA twist), which breaks histone-DNA contacts and propagates as a wave of DNA around the octamer. The demonstration of DNA translocation presented here provides a mechanistic basis for this DNA wave. To test the relative contribution of twist to remodeling, we use nucleosomes containing nicks in precise locations to uncouple twist and translocation. Nucleosomes bearing nicks are remodeled less efficiently than intact nucleosomes. These results suggest that RSC and Sth1 are DNA translocases that use both DNA translocation and twist to remodel nucleosomes efficiently.

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“…As EcoR124I is a restriction endonuclease, it is conceivable that the difference in rate corresponds to the cleavage state of the DNA. ATP would be hydrolyzed rapidly while translocating on scDNA against the generation of twist, 15,25 and once cleavage occurred, the topological constraint would be removed and the rate of ATP hydrolysis would decrease accordingly.…”

Section: The Effects Of Magnesium Ions On Atpase Activity Of Ecor124imentioning

confidence: 99%