2019
DOI: 10.1177/2472555218791743
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DNA Switch: Toehold-Mediated DNA Isothermal Amplification for Dengue Serotyping

Abstract: The inherent ability of nucleic acids to recognize a complementary pair has gained wide popularity in DNA sensor applications. DNA molecules can be produced in bulk and easily incorporated with various nanomaterials for sensing applications. More complex designs and sophisticated DNA sensors have been reported over the years to allow DNA detection in a faster, cheaper, and more convenient manner. Here, we report a DNA sensor designed to function like a switch to turn "on" silver nanocluster (AgNC) generation i… Show more

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Cited by 4 publications
(6 citation statements)
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“…We plotted the intensity at 2 h interval against the complex concentration and obtained a linear fit between 10 nM to 100 nM with a goodness-of-fit R 2 = 0.9993. This showed that the model is sensitive to changes in the concentration of the trigger miRNA biomarker, satisfying a necessary biosensor property [50][51][52]. We repeated the process for a time interval of 4h where a similar trend was obtained (R 2 = 0.9992) (Fig.…”
Section: Generalization To Second Generation Toehold Switchesmentioning
confidence: 58%
“…We plotted the intensity at 2 h interval against the complex concentration and obtained a linear fit between 10 nM to 100 nM with a goodness-of-fit R 2 = 0.9993. This showed that the model is sensitive to changes in the concentration of the trigger miRNA biomarker, satisfying a necessary biosensor property [50][51][52]. We repeated the process for a time interval of 4h where a similar trend was obtained (R 2 = 0.9992) (Fig.…”
Section: Generalization To Second Generation Toehold Switchesmentioning
confidence: 58%
“…Whole blood and plasma were spiked with cultured isolates of each DENV serotype to replicate viral loads observed in acute infection. 5,6,23 As shown in Figure 6, all samples gave positive results. Following dilution of the eluates, estimated viral loads in the higher-concentration mock samples were consistent with or lower than viral loads that are commonly observed during the acute phase of dengue.…”
Section: ■ Discussionmentioning
confidence: 75%
“…Here, we evaluated the detection of DENV RNA in 16 spiked clinical samples. Whole blood and plasma were spiked with cultured isolates of each DENV serotype to replicate viral loads observed in acute infection. ,, As shown in Figure , all samples gave positive results. Following dilution of the eluates, estimated viral loads in the higher-concentration mock samples were consistent with or lower than viral loads that are commonly observed during the acute phase of dengue. , As all low-concentration samples were positive, the technology in this study detected DENV RNA concentrations that are at least 100-fold lower than values commonly observed in the first days of illness.…”
Section: Discussionmentioning
confidence: 97%
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“…The plot of intensity against the complex concentration over a 2-h interval obtained a sigmoidal fit with a steep rise between 10 nM and 100 nM (Section S4, Supplementary File S7). This showed that the model is sensitive to changes in the concentration of the trigger miRNA biomarker in the range of interest, satisfying a necessary biosensor property [ 25 , [55] , [56] , [57] , [58] , [59] , [60] ]. A similar trend of sigmoidal kinetics was observed for the hsa-miR-20a-5p biomarker over a complex concentration range of 100 nM and 1 μM (Supplementary File S7).…”
Section: Resultsmentioning
confidence: 99%