DNA Synthesis in the Tracheae of Aging Mice by Light and Electron Microscopic Radioautography.

Sun, Lin; Gao, Fang; Jin, Chunji; Nagata, T.

doi:10.1267/ahc.30.211

Cited by 23 publications

(28 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…13B). The labeling indices of respective cell types were analyzed (Sun et al 1997a). As the results, the labeling indices of the epithelial cells showed their maxima on fetal day 18, then fell down from postnatal day 3 to 2 years (Fig.…”

Section: The Dna Synthesis In the Tracheamentioning

confidence: 94%

“…The changes of DNA synthesis of tracheal cells in aging mice were studied by LM and EMRAG (Sun et al 1997a, Nagata 2000d. The tracheae of 8 groups of mice from fetal day 18 to 2 years after birth were examined.…”

Section: The Dna Synthesis In the Tracheamentioning

confidence: 99%

“…When the lung tissues of mice were labeled with 3 H-uridine, RNA synthesis was observed in all cells of the lungs at various ages by RAG (Sun 1995, Sun et al 1997b, Nagata 2002. Observing the light microscopic radioautograms labeled with 3 H-uridine, the silver grains were found over both the karyoplasm and cytoplasm of almost all the cells not only at the perinatal stages from embryo day 16, 18, to postnatal day 1, 3, 7, 14, but also at the adult and senescent stages from postnatal month 1 to 2, 6, 12 and 22.…”

Section: Rna Synthesis Of Mouse Pulmonary Cellsmentioning

confidence: 99%

“…By electron microscopic radioautography, silver grains were observed in most pulmonary cells in respective aging groups localizing not only over euchromatin and nucleoli in the nuclei but also over many cell organelles such as endoplasmic reticulum, ribosomes, and mitochondria as well as cytoplasmic matrices from perinatal stage at embryonic day 16, 18, to postnatal day 1, 3, 7, 14, to adult and senescent stages at postnatal month 1, 2, 6, 12 and 22 (Sun 1995, Sun et al 1997b, Nagata 2002. The localizations of silver grains over the mitochondria were mainly on the mitochondrial matrices but a few over the mitochondrial membranes and cristae when observed by high power magnification.…”

Section: The Rna Synthesis Of Mitochondria In Pulmonary Cellsmentioning

confidence: 99%

See 3 more Smart Citations

Macromolecular Synthesis in the Digestive and Respiratory Systems

Nagata¹

2012

Senescence

Self Cite

View full text Add to dashboard Cite

IntroductionThis second chapter deals with the second parts of the application of microscopic radioautography to some of the visceral organ systems. The visceral organs can be divided into 5 organ systems according to anatomy and histology, i.e., the digestive system, the respiratory system, the urinary system, the reproductive system and the endocrine system. Among of them the digestive system consists of 2 parts, i.e., the digestive tract and the digestive glands. The former consists of simple tube structures such as the oral cavity, the esophagus, the stomach and the intestines, while the latter consists of complicated glandular structures such as the large digestive glands , i.e., the liver and the pancreas, while the respiratory system consists of 2 parts, one the respiratory tract such as the nose, the pharynx, the trachea, the bronchus, and final essential part the lungs. This chapter deals with the digestive organs and the respiratory organs, respectively. Macromolecular synthesis in the digestive systemThe digestive system consists of the digestive tract and the digestive glands. The digestive tract can be divided into several portions, from the upper part to the lower part, i.e., the oral cavity, the pharynx, the esophagus, the stomach, the small and large intestines and the anus, while the digestive glands consist of the large glands such as the salivary glands, the liver and the pancreas and the small glands affiliated to the digestive tracts in the gastrointestinal walls such as the gastric glands including the fundic gland and the pyloric gland, the intestinal glands of Lieberkühn and the duodenal glands of Brunner. We have published many papers from our laboratory dealing with the macromolecular synthesis in respective digestive organs from the oral cavity to the gastrointestinal tracts and the digestive glands

show abstract

Section: The Dna Synthesis In the Tracheamentioning

confidence: 94%

Section: The Dna Synthesis In the Tracheamentioning

confidence: 99%

Section: Rna Synthesis Of Mouse Pulmonary Cellsmentioning

confidence: 99%

Section: The Rna Synthesis Of Mitochondria In Pulmonary Cellsmentioning

confidence: 99%

See 2 more Smart Citations

Macromolecular Synthesis in the Digestive and Respiratory Systems

Nagata¹

2012

Senescence

Self Cite

View full text Add to dashboard Cite

show abstract

“…It was later commonly found in various cells and tissues not only in vitro obtained from various organs in vivo (Nagata, 1984;Nagata and Murata, 1977;Nagata et al, 1977a-c), but also in vivo cells of various organs such as the salivary glands (Nagata et al, 2000), the liver (Nagata et al, 1979(Nagata et al, , 1982aMa and Nagata, 1988;Ma et al, 1994), the pancreas (Nagata, 1992;Nagata et al, 1986), the trachea (Sun et al, 1997), the kidneys (Hanai et al, 1994), the testis (Gao et al, , 1995, the uterus (Yamada and Nagata, 1994a,b), the adrenals (Ito and Nagata, 1996;Liang et al, 1999), the brains , the retina (Gunarso, 1984;Gunarso et al, 1996Gunarso et al, , 1997Kong and Nagata, 1994) of mice, rats, and chickens. The relationship between the intramitochondrial RNA synthesis and cell cycle was formerly studied and it was clarified that the intramitochondrial DNA synthesis was performed without nuclear involvement (Nagata, 1972b).…”

Section: Introductionmentioning

confidence: 99%

Electron microscopic radioautographic study of RNA synthesis in hepatocyte mitochondria of aging mouse

Nagata

2005

Microsc. Res. Tech.

Self Cite

View full text Add to dashboard Cite

In order to study the aging changes of intramitochondrial RNA synthesis in mouse hepatocytes, 10 groups of aging mice, each consisting of three individuals (total 30) from fetal day 19 to postnatal month 24 were injected with 3 H-uridine, an RNA precursor, sacrificed 1 hour later, and the liver tissues processed for electron microscopic radioautography. On EM radioautograms obtained from each animal the number of mitochondria, the number of labeled mitochondria, and the mitochondrial labeling index labeled with 3 H-uridine showing RNA synthesis in each hepatocytes, both mononucleate and binucleate cells, were counted and the averages in respective aging groups were compared. From the results it was demonstrated that the numbers of mitochondria, the numbers of labeled mitochondria, and the labeling indices of intramitochondrial RNA syntheses in both mononucleate and binucleate hepatocytes of mice at various ages increased and decreased according to the age of the animals. INTRODUCTIONIntramitochondrial nucleic acid syntheses, both DNA and RNA, in mammalian and avian cells were first demonstrated morphologically by the present authors by means of electron microscopic radioautography with accurate localization in primary cultured cells of the livers and kidneys of mice and chickens in vitro (Nagata et al., 1967a,b) and then in some other established cell lines such as HeLa cells (Nagata, 1972a-d) or mitochondrial fractions prepared from in vivo cells (Nagata, 1974;Nagata et al., 1975Nagata et al., , 1976. It was later commonly found in various cells and tissues not only in vitro obtained from various organs in vivo (Nagata, 1984; Nagata and Murata, 1977; Nagata et al., 1977a-c), but also in vivo cells of various organs such as the salivary glands (Nagata et al., 2000), the liver (Nagata et al., 1979(Nagata et al., , 1982a Ma and Nagata, 1988;Ma et al., 1994), the pancreas (Nagata, 1992;Nagata et al., 1986), the trachea (Sun et al., 1997), the kidneys (Hanai et al., 1994), the testis (Gao et al., , 1995, the uterus (Yamada and Nagata, 1994a,b), the adrenals (Ito and Nagata, 1996;Liang et al., 1999), the brains , the retina (Gunarso, 1984;Gunarso et al., 1996Gunarso et al., , 1997Kong and Nagata, 1994) of mice, rats, and chickens. The relationship between the intramitochondrial RNA synthesis and cell cycle was formerly studied and it was clarified that the intramitochondrial DNA synthesis was performed without nuclear involvement (Nagata, 1972b). However, the relationship between RNA synthesis and the aging of individual animals has not yet been clarified. This article deals with the relationship between RNA synthesis and aging in hepatocytes of mice in vivo at various ages by means of electron microscopic radioautography as part of a serial studies on special cytochemistry (Nagata, 2001) and radioautographology (Nagata, 2002).

show abstract

Macromolecular synthesis in the livers of aging mice as revealed by electron microscopic radioautography

Nagata

2010

Progress in Histochemistry and Cytochemistry

View full text Add to dashboard Cite

DNA Synthesis in the Tracheae of Aging Mice by Light and Electron Microscopic Radioautography.

Cited by 23 publications

References 31 publications

Macromolecular Synthesis in the Digestive and Respiratory Systems

Macromolecular Synthesis in the Digestive and Respiratory Systems

Electron microscopic radioautographic study of RNA synthesis in hepatocyte mitochondria of aging mouse

Macromolecular synthesis in the livers of aging mice as revealed by electron microscopic radioautography

Contact Info

Product

Resources

About