DNA tetrahedron-based CRISPR bioassay for treble-self-amplified and multiplex HPV-DNA detection with elemental tagging

Atomic spectrometry update: review of advances in the analysis of clinical and biological materials, foods and beverages

“…Elemental tagging for biological detection and imaging continues to be an expanding area of interest, with the emergence of various novel platforms for multiplexing in nucleic acid-based diagnostics . In the method developed by Zhan et al , 194 multiplexed HPV-DNA detection was achieved using a clustered regularly interspaced short palindromic repeats (CRISPR) platform with DNA tetrahedron (DTN) functionalised magnetic beads (MBs) and tagged metal NP probes. NP-bound linker probes identified by the CRISPR system were cleaved, preventing hybridisation with DTN capture probes on MBs, thus allowing for ICP-MS detection following magnetic separation.…”

Multiplexed Detection Strategies for Biosensors Based on the CRISPR-Cas System

“…To obtain high sensitivity and short detection time, Zhan et al proposed a DNA tetrahedron (DNT) based CRISPR biosensor for simultaneous detection of multiplex HR-HPV-DNA (Figure 4D). 98 Different space separated target DNAs were recognized by Cas12a to activate its trans-cleavage activity to cleave the surrounding corresponding ssDNA linker into fragments. Therefore, the metalnanoparticle probes were unable to bind to the DTN-modified magnetic bead probes (MBsDTN), resulting in significant inductively coupled plasma mass spectrometry (ICP-MS) signal changes.…”

“…(D) The working principle of DNA tetrahedron based CRISPR biosensor for simultaneous detection of multiplex HR-HPV-DNA. Reproduced from ref . Copyright 2023 Elsevier B.V.…”

“…The combination of QDs and CRISPR based detection broadens its application. To obtain high sensitivity and short detection time, Zhan et al proposed a DNA tetrahedron (DNT) based CRISPR biosensor for simultaneous detection of multiplex HR-HPV-DNA (Figure D) . Different space separated target DNAs were recognized by Cas12a to activate its trans-cleavage activity to cleave the surrounding corresponding ssDNA linker into fragments.…”

PER-CRISPR/Cas14a system-based electrochemical biosensor for the detection of ctDNA EGFR L858R

“…32,33 In the CRISPR/Cas family, CRISPR/Cas12a is a superstar for application in biosensors. It can be used in the detection of nucleic acids coupled with other technologies, such as hybridization chain reaction, 34 inductively coupled plasma mass spectrometry 35 and strand displacement/hybridization chain reaction. 36 CRISPR/Cas14a, as an endonuclease, has been used for the construction of an electrochemical biosensor, [37][38][39] which displayed high specicity, sensitivity and cleavage efficiency, revealing enormous potential in nucleic acid detection.…”