DNAzyme recognition triggered cascade signal amplification for rapid and highly sensitive visual detection of uranyl ions

Abstract

This work presents a rapid and highly sensitive colorimetric assay using bifunctional DNA probe decorated agarose microbeads (MBs) coupled with a cascade signal amplification system, including rolling circle amplification (RCA)...

A cascade signal-amplified fluorescent biosensor combining APE1 enzyme cleavage-assisted target cycling with rolling circle amplification

“…Additionally, the rolling circle amplification (RCA) reaction is an isothermal amplification technique employed for DNA and RNA replication by producing DNA segments with repetitive units, which play a signal amplification role in nucleic acid biosensors with improved sensitivity. 21–25 However, in most biosensors where the RCA reaction is triggered directly by the target miRNA as the primer, only single signal amplification is achieved. 26 To address this limitation, we propose triggering the rolling circle amplification reaction using the primer released from the preceding target cycle reaction.…”

Colorimetric sensing for the sensitive detection of UO22+via the phosphorylation functionalized mesoporous silica-based controlled release system

“…Zhang et al 23 also developed a simple portable colorimetric sensing device for UO 2 2+ detection in aqueous solvents based on vinyl phosphonic acid functionalized gold nanoparticles. Zhang et al 24 used DNAzyme-decorated agarose beads to form a coupled cascade signal amplification system to realize rapid and high-sensitivity visual detection of UO 2 2+ in water. The above methods exhibited high sensitivity and specificity for UO 2 2+ detection.…”