Do environmentally relevant concentrations of fluoxetine and citalopram impair stress-related behavior in zebrafish (Danio rerio) embryos?

Zindler, Florian; Stoll, Saskia; Baumann, Lisa; Knoll, Sarah; Hühn, Carolin; Braunbeck, Thomas

doi:10.1016/j.chemosphere.2020.127753

Cited by 28 publications

(23 citation statements)

References 68 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After 21 days of exposure, the change in locomotor activity during the light period became more evident. In a study carried out by Zindler et al (2020) [60], fluoxetine reduced the swimming distance of embryos in the dark and changed the maximum speed during light periods, after 96 h exposure. Thigmotaxis is seen as the tendency of an animal placed in a new environment, to remain close to the walls and avoid the center of the aquarium.…”

Section: Discussionmentioning

confidence: 95%

“…After 21 days of exposure, the change in locomotor activity during the light period became more evident. In a study carried out by Zindler et al, (2020) [60], fluoxetine reduced the swimming distance of embryos in the dark and changed the maximum speed during light periods, S6). Asterisks (*) indicate differences towards the control (One Way ANOVA, Dunnett's Method, p < 0.05).…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have demonstrated that fluoxetine can be toxic to fish, with exposure resulting in changes at different biological levels, from gene transcription, neurotransmission markers, enzymatic activities (e.g., oxidative stress, metabolism), hormone levels, reproductive processes, and accumulation in various tissues (e.g., brain and liver), resulting in a severe change in the histology of these organs [4][5][6][7]10,11,14,[40][41][42][43][44][45][46][47][48]. In addition, this pharmaceutical can cause changes in behavior (e.g., locomotor activity, stress response, feeding, aggression, social and anti-predatory behavior) [4][5][6][7][8][9]11,15,16,45,[49][50][51][52][53][54][55][56][57][58][59][60].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Chronic Effects of Fluoxetine on Danio rerio: A Biochemical and Behavioral Perspective

et al. 2022

View full text Add to dashboard Cite

Fluoxetine is an antidepressant widely used to treat depressive and anxiety states. Due to its mode of action in the central nervous system (selective serotonin reuptake inhibitor (SSRI)), it becomes toxic to non-target organisms, leading to changes that are harmful to their survival. In this work, the effects of fluoxetine on juvenile zebrafish (Danio rerio) were evaluated, assessing biochemical (phase II biotransformation—glutathione S-transferase (GST), neurotransmission—acetylcholinesterase (ChE), energy metabolism—lactate dehydrogenase (LDH), and oxidative stress—glutathione peroxidase (GPx)) and behavior endpoints (swimming behavior, social behavior, and thigmotaxis) after 21 days exposure to 0 (control), 0.1, 1 and 10 µg/L. Biochemically, although chronic exposure did not induce significant effects on neurotransmission and energy metabolism, GPx activity was decreased after exposure to 10 µg/L of fluoxetine. At a behavioral level, exploratory and social behavior was not affected. However, changes in the swimming pattern of exposed fish were observed in light and dark periods (decreased locomotor activity). Overall, the data show that juvenile fish chronically exposed to fluoxetine may exhibit behavioral changes, affecting their ability to respond to environmental stressors and the interaction with other fish.

show abstract

Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Chronic Effects of Fluoxetine on Danio rerio: A Biochemical and Behavioral Perspective

et al. 2022

View full text Add to dashboard Cite

show abstract

“…In line with the developmental time line, the nervous system is assumed to be fully developed only by 10 days post-fertilization (de Esch et al 2012 ). This implies that in case compounds tested in the FET test are likely to induce severe neurodevelopmental effects, only part of the endpoints of neurotoxicity may be observable in 96-h-old embryos (Zindler et al 2019a , b , 2020a , b). For some endpoints (such as swimming assays and anxiety tests), specific test setups might be required (Selderslaghs et al 2009 , 2012 , 2013 ; Zindler et al 2019a , 2020a , b ).…”

Section: Resultsmentioning

confidence: 99%

“…This implies that in case et al 2019a, b, 2020a, b). For some endpoints (such as swimming assays and anxiety tests), specific test setups might be required (Selderslaghs et al 2009(Selderslaghs et al , 2012(Selderslaghs et al , 2013Zindler et al 2019aZindler et al , 2020a.…”

Section: Time-dependent Toxicity Profilementioning

confidence: 99%

Specificity of time- and dose-dependent morphological endpoints in the fish embryo acute toxicity (FET) test for substances with diverse modes of action: the search for a “fingerprint”

Hellfeld

Pannetier

Braunbeck

2021

Environ Sci Pollut Res

Self Cite

View full text Add to dashboard Cite

The fish embryo acute toxicity (FET) test with the zebrafish (Danio rerio) embryo according to OECD TG 236 was originally developed as an alternative test method for acute fish toxicity testing according to, e.g., OECD TG 203. Given the versatility of the protocol, however, the FET test has found application beyond acute toxicity testing as a common tool in environmental hazard and risk assessment. Whereas the standard OECD guideline is restricted to four core endpoints (coagulation as well as lack of somite formation, heartbeat, and tail detachment) for simple, rapid assessment of acute toxicity, further endpoints can easily be integrated into the FET test protocol. This has led to the hypothesis that an extended FET test might allow for the identification of different classes of toxicants via a “fingerprint” of morphological observations. To test this hypothesis, the present study investigated a set of 18 compounds with highly diverse modes of action with respect to acute and sublethal endpoints. Especially at higher concentrations, most observations proved toxicant-unspecific. With decreasing concentrations, however, observations declined in number, but gained in specificity. Specific observations may at best be made at test concentrations ≤ EC10. The existence of a “fingerprint” based on morphological observations in the FET is, therefore, highly unlikely in the range of acute toxicity, but cannot be excluded for experiments at sublethal concentrations.

show abstract

The sensitivity of the zebrafish embryo coiling assay for the detection of neurotoxicity by compounds with diverse modes of action

Hellfeld

Gade

Baumann

et al. 2023

Environ Sci Pollut Res

Self Cite

View full text Add to dashboard Cite

In the aim to determine neurotoxicity, new methods are being validated, including tests and test batteries comprising in vitro and in vivo approaches. Alternative test models such as the zebrafish (Danio rerio) embryo have received increasing attention, with minor modifications of the fish embryo toxicity test (FET; OECD TG 236) as a tool to assess behavioral endpoints related to neurotoxicity during early developmental stages. The spontaneous tail movement assay, also known as coiling assay, assesses the development of random movement into complex behavioral patterns and has proven sensitive to acetylcholine esterase inhibitors at sublethal concentrations. The present study explored the sensitivity of the assay to neurotoxicants with other modes of action (MoAs). Here, five compounds with diverse MoAs were tested at sublethal concentrations: acrylamide, carbaryl, hexachlorophene, ibuprofen, and rotenone. While carbaryl, hexachlorophene, and rotenone consistently induced severe behavioral alterations by ~ 30 h post fertilization (hpf), acrylamide and ibuprofen expressed time- and/or concentration-dependent effects. At 37–38 hpf, additional observations revealed behavioral changes during dark phases with a strict concentration-dependency. The study documented the applicability of the coiling assay to MoA-dependent behavioral alterations at sublethal concentrations, underlining its potential as a component of a neurotoxicity test battery.

show abstract

Do environmentally relevant concentrations of fluoxetine and citalopram impair stress-related behavior in zebrafish (Danio rerio) embryos?

Cited by 28 publications

References 68 publications

Chronic Effects of Fluoxetine on Danio rerio: A Biochemical and Behavioral Perspective

Chronic Effects of Fluoxetine on Danio rerio: A Biochemical and Behavioral Perspective

Specificity of time- and dose-dependent morphological endpoints in the fish embryo acute toxicity (FET) test for substances with diverse modes of action: the search for a “fingerprint”

The sensitivity of the zebrafish embryo coiling assay for the detection of neurotoxicity by compounds with diverse modes of action

Contact Info

Product

Resources

About