Does allicin combined with vitamin B-complex have superior potentials than alpha-tocopherol alone in ameliorating lead acetate-induced Purkinje cell alterations in rats? An immunohistochemical and ultrastructural study

Mustafa, Hesham N.; Hussein, Adel M.

doi:10.5603/fm.a2015.0076

Cited by 17 publications

(7 citation statements)

References 47 publications

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“…Additionally, degenerating pyknotic cells and vacuolation in the Purkinje cell layer were easily detected in the Pb group. Similarly, previous studies reported that Pb exposure during cerebellar development significantly decreased the number of Purkinje cells and ascorbic acid treatment mitigated Pb-induced impairments in the PA group [17,20,34,35,36]. Our results are consistently similar with previous studies, which observed that ascorbic acid prevents the harmful effects of other heavy metals including mercury and cadmium on brain development [37,38].…”

Section: Discussionsupporting

confidence: 92%

Effects of Ascorbic Acid on Osteopontin Expression and Axonal Myelination in the Developing Cerebellum of Lead-Exposed Rat Pups

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Seo

Nahm

et al. 2019

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Osteopontin (OPN) is a multi-functional protein that binds to integrin and calcium-binding phosphoprotein. OPN is required for normal neuronal development and its axonal myelination. We studied the combined effect of lead (Pb) and ascorbic acid treatment on OPN expression in the developing cerebellum. We randomly divided pregnant female rats into three groups: control, Pb (lead acetate, 0.3%, drinking water), and Pb plus ascorbic acid (PA; ascorbic acid, 100 mg/kg, oral intubation) groups. The blood level of Pb was significantly increased, while ascorbic acid reduced Pb levels in the dams and pups. At postnatal day (PND) 21, results from Nissl staining and OPN immunohistochemistry demonstrated that OPN was detected in the Purkinje cell layer in the cerebellum. Ascorbic acid treatment mitigated Pb exposure-induced reduction in the number of intact Purkinje cells and OPN immunoreactive Purkinje cells in the cerebellum of pups. In addition, Pb-induced reduction in the number of oligodendrocytes and myelin-associated glycoprotein is associated with the malformation of the myelin sheath. Ascorbic acid provided protection from Pb-induced impairments. Pb-induced structural deficits in the cerebellum resulted in functional deterioration observed during locomotive tests (bar holding test and wire mesh ascending test), while ascorbic acid ameliorated these harmful effects. Present results suggest that the change of OPN is associated with myelination in the developing cerebellum. The results also demonstrated that exposure to Pb is harmful, while ascorbic acid treatment is beneficial.

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Section: Discussionsupporting

confidence: 92%

Effects of Ascorbic Acid on Osteopontin Expression and Axonal Myelination in the Developing Cerebellum of Lead-Exposed Rat Pups

Nam

Seo

Nahm

et al. 2019

IJERPH

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“…For this reason, the cerebellum is especially vulnerable to developmental damages on exposure to oxidative stress [12,20,23,28,42]. reactive oxygen species in the CNS [33]. Another study described that allicin alters the GFAP content in various brain regions and has a principal neuroprotective influence in minimising neuroglia injury in the CNS [9]; in such a basis, the usage of garlic in the current study diminished GFAP expression in rats' cerebellum.…”

Section: Discussionmentioning

confidence: 51%

“…GFAP positive cells appeared brown and nuclei appeared blue. The positive results were indicated by brown coloration of the cell membrane and cytoplasm of the astrocytes [33]. Finally, all slides were examined with an Olympus BX53 microscope equipped with camera (Olympus, Tokyo, Japan) at different magnifications.…”

Section: Histological and Immunohistochemical Methodsmentioning

confidence: 99%

Protective effect of garlic extract against maternal and foetal cerebellar damage induced by lead administration during pregnancy in rats

et al. 2018

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This study was useful in evaluating the hazardous effects of uncontrolled use of Pb in general and in assessing the developmental and neurotoxicity of foetuses due to exposure during pregnancy in particular. Co-administration of garlic has beneficial effects in amelioration of Pb-induced neurotoxicity and reversing the histopathological changes of the cerebellum of mother rats and foetuses. (Folia Morphol 2018; 77, 1: 1-15).

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“…Group II (LA treated group) (positive control [PC]) was given 10 mg/kg of LA, orally for 30 days [24,25]. Group III (NS treated group) was given 200 mg/kg BW of NS water suspension, orally for 30 days [26,27], while Group IV (NS followed by LA treated group) was pre-treated with NS 200 mg/kg BW, orally for 30 days [13], followed by LA 10 mg/kg BW daily for another 30 days [24,25]. …”

Section: Methodsmentioning

confidence: 99%

Prophylactic effect of Nigella sativa against lead acetate induced changes in spermiogram, reproductive hormones and gonadal histology of rats

et al. 2016

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Aim:This study was designed to evaluate the prophylactic effect of Nigella sativa (NS) treatment on toxic effects induced by lead acetate (LA) on the reproductive hormones, spermiogram and gonadal histology of rats.Materials and Methods:A total of 20 Sprague-Dawley rats were divided into four groups of five rats each. Group 1 (negative control [NC]) was the NC and was given distilled water, Group 2 served as the positive control (PC) and was administered 10 mg/kg/day of LA per overall survival (OS), Group 3 (T1) was administered 200 mg/kg/daily of NS per OS for a month, and Group 4 (T2) was pretreated with 200 mg/kg/daily of NS per OS for 1 month, followed by 10 mg/kg/daily of LA alone per OS for another. The rats were euthanized at the end of the experimental period for collection of blood and the right caudal epididymis and testis. Serum was used for determination of reproductive hormones by using radioimmunoassay kits. The epididymal segment was cut and homogenized in phosphate-buffered saline, and the homogenate was used for determination of the spermiogram parameters such as sperm concentration, sperm viability, percentage of live sperm, motility and abnormality. Both the epididymis and testis were fixed in 10% buffered formalin for histological processing.Results:The sperm concentration, general, and individual motilities were higher (p<0.05) in the NC and T1 animals, while the T2 had intermediate and the PC had lower (p<0.05) values of each parameter. The percentage sperm viability was higher (p<0.05) in the T1 and lower (p<0.05) in the PC group. However, percentage abnormality was lower in T1, comparable in NC and T2, and higher (p<0.05) in PC. Spermatogenic cell population and epididymal sperm reserve (ESR) were optimal in control and pretreated animals, while the PC had lower spermatids and ESR. The concentration of estradiol (EST) was lower (p<0.05) in the PC and T2, while leuteinizing hormone (LH) concentration was lower (p<0.05) in the PC, and comparable (p>0.05) between control and T2. The concentration of follicle-stimulating hormone (FSH) was comparable (p>0.05) in all groups, while testosterone (TS) hormone concentration was lower (p<0.05) in the PC and higher in the control and T1 groups.Conclusion:This study showed the preventive effects of NS administration against alterations in reproductive hormnes, sperm parameters and gonadal histology caused by LA in rats.

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Does allicin combined with vitamin B-complex have superior potentials than alpha-tocopherol alone in ameliorating lead acetate-induced Purkinje cell alterations in rats? An immunohistochemical and ultrastructural study

Abstract: (Folia Morphol 2016; 75, 1: 76-86)

Cited by 17 publications

References 47 publications

Effects of Ascorbic Acid on Osteopontin Expression and Axonal Myelination in the Developing Cerebellum of Lead-Exposed Rat Pups

Effects of Ascorbic Acid on Osteopontin Expression and Axonal Myelination in the Developing Cerebellum of Lead-Exposed Rat Pups

Protective effect of garlic extract against maternal and foetal cerebellar damage induced by lead administration during pregnancy in rats

Prophylactic effect of Nigella sativa against lead acetate induced changes in spermiogram, reproductive hormones and gonadal histology of rats

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