Does casomorphin have a functional role?

Abstract

Degradation of buffalo &casein by various physioIogica1 enzymes was studied. Digestion with gastric and pancreatic proteases plus leucine aminopeptidase did not release casomorphins but a putative precursor (procasomorphin) which was further digested by brush border peptidases into peptides differing from casomorphins. CasomorphinBrush border protease Casein sigh-performance Iiquid chromatography

Peptides du lait à activité biologique

“…3). Si les caséines as et f3 apparaissent comme les principales sources de ce type de peptides, que ce soit dans le lait humain ou dans le lait bovin : des séquences proches voire identiques ont également été retrouvées dans les caséines de brebis (Richardson & Mercier 1979), de bufflesse (Petrilli et al, 1984) et de chamelle (Beg et al, 1986), il est à noter qu'ils sont également présents dans la séquence des deux principales protéines du lactosé-rum : l'a-lactalbumine de la {3-lactoglobuline (Fig. 3).…”

Peptides surviving the simulated gastrointestinal digestion of milk proteins: Biological and toxicological implications

“…For this reason, milk protein degradation in newborns is rather variable and products of digestion are somewhat complex to be rationalized. Petrilli et al [23] using an in vitro digestion model, which included a final hydrolysis step by using BBM, demonstrated that ␤-casomorphins did not survive the digestive degradation of buffalo ␤-casein (␤-CN). Similarly, pig pancreatic juice did not release ␤-casomorphins from a pro-␤-casomorphin tryptic peptide [24].…”

Isolation and characterization of dipeptidyl peptidase IV from human meconium

“…Purified DAP-IV was incubated with neuraminidase according to [23] or to [24]. Casomorphin-9 was obtained, by Edman degradation, from procasomorphin which was purified as in [15]. FAB mass spectra were recorded on a double-focusing VG ZAB HF mass spectrometer equipped with a FAB source of the same company and an M-Scan gun.…”