Does Cisapride, as a 5HT₄Receptor Agonist, Aggravate the Severity of TNBS-Induced Colitis in Rat?

Abstract

There is a pressing need for research that will lead to the reveal of targets designed to analyse the possible pathways for the treatment of IBD. Because of the probable involvement of serotonin in inflammatory conditions of intestine and the important role of 5HT4 receptors in GI function, the investigation of the role of 5HT4 receptors in the pathogenesis of IBD will be interesting. The aim of this study was to investigate the effects of cisapride, a 5HT4 receptor agonist, in trinitrobenzenesulfonic-acid-(TNBS) induced rat colitis. Two hours subsequent to induction of colitis using TNBS in rats, cisapride (2 mg/kg, intraperitoneally (i.p); 4 mg/kg, orally (p.o)) and dexamethasone (1 mg/kg, i.p; 2 mg/kg, p.o) were administrated for 6 days. Animals were thereafter euthanized; macroscopic, histological, and biochemical assessments and ELISA test were carried out on distal colon samples. Our data showed that dexamethasone treatment (i.p, p.o) significantly decreased macroscopic and microscopic damage and also biochemical markers, but there were no significant differences in aforementioned parameters between cisapride (i.p or p.o) and TNBS-treated rats. It can be deduced that because the severity of colitis produced by TNBS is massive (through various pathways), cisapride could not bring about more colitis damages through 5HT4 receptors. Based on the present study further researches are required for investigating the exact roles of 5HT4 receptors in the pathogenesis of ulcerative colitis.

Anti-inflammatory effects of alosetron mediated through 5-HT3 receptors on experimental colitis

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Beneficial Effects of Maprotiline in a Murine Model of Colitis in Normal and Reserpinised Depressed Rats

“…Then paraffin sections were deparaffinized with xylene and then hydrated and stained with hematoxylin and eosin (H&E). The stained sections were assessed for any inflammatory changes including infiltration of cells, necrosis, or damage to tissue structures [ 31 ]. Inflammation severity and extent as well as crypt damage were evaluated on H&E stained according to the criteria previously described by Dieleman et al [ 32 ].…”

Electroacupuncture at ST25 Inhibits Cisapride‐Induced Gastric Motility in an Intensity‐Dependent Manner

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