Does concentration influence viability of the bronchial epithelial cell line chronically exposed to antiasthmatic drugs?

Abstract

A Ad dd dr re es ss s f fo or r c co or rr re es sp po on nd de en nc ce e: : Dominika Oleś MA, Laboratory of Molecular and Cell Biology, Poznan University of Medical Sciences, 27/33 Szpitalna St, 60-572 Poznan, Poland, phone: +48 61 849 13 37, fax: +48 61 848 01 11, e-mail: dominikaol@onet.eu Observations were performed every 24 h for 4 days. Cells viability was analyzed by fluorescent staining and XTT assay. R Re es su ul lt ts s: : An inverse correlation between drug concentration and cells viability was observed. The only exception was ipratropium bromide which was toxic at all studied concentrations. Steroids at the two highest concentrations led to a significant decrease in cells viability with fluticasone propionate being more potent than budesonide. Incubation with salbutamol also demonstrated decreased cells viability at the two highest concentrations. Ipratropium bromide was toxic for the bronchial cells at all concentrations leading to a significant decrease in cells viability. C Co on nc cl lu us si io on ns s: : Chronic exposure to the highest concentrations of steroids or β 2 -agonists decreases viability of epithelial cells, whereas ipratropium bromide has the strongest influence on cell viability regardless of its concentration. K Ke ey y w wo or rd ds s: : bronchial epithelial cells, bronchodilators, drug concentration, viability, steroids.

Experimental research The effects of budesonide on angiogenesis in a murine asthma model

“…Common medications used to treat asthma are glucocorticoids, leukotriene receptor antagonists and theophylline [4]. And corticosteroids are the most effective drugs to suppress airway inflammation, mainly by reducing pro-inflammatory cytokines [5–7]. In addition, corticosteroids seem to reverse asthma-induced airway remodeling, including the increased vascularity of the bronchial wall [8], partly through regulating target genes to suppress the vascular elements of angiogenesis [9].…”