Does corazonin signal nutritional stress in insects?

Veenstra, Jan A.

doi:10.1016/j.ibmb.2009.09.008

Cited by 88 publications

(91 citation statements)

References 92 publications

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“…Specifically, CCAP and bursicon are key players in ecdysis and cuticular tanning (Arakane et al, 2008;Honegger et al, 2008;Lahr et al, 2012). The role of corazonin in molting is less clear (Veenstra, 2009;Boerjan et al, 2010), but this neurohormone regulates the melanization patterns associated with the gregarious phase of migratory locusts (Tawfik Fig .7. Effect of CCAP gene knockdown on mosquito heart physiology.…”

Section: Discussionmentioning

confidence: 99%

Cardioacceleratory function of the neurohormone CCAP in the mosquito Anopheles gambiae

Estévez‐Lao

Boyce

Honegger

et al. 2013

Journal of Experimental Biology

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SUMMARYCrustacean cardioactive peptide (CCAP) is a highly conserved arthropod neurohormone that is involved in ecdysis, hormone release and the modulation of muscle contractions. Here, we determined the CCAP gene structure in the malaria mosquito Anopheles gambiae, assessed the developmental expression of CCAP and its receptor and determined the role that CCAP plays in regulating mosquito cardiac function. RACE sequencing revealed that the A. gambiae CCAP gene encodes a neuropeptide that shares 100% amino acid identity with all sequenced CCAP peptides, with the exception of Daphnia pulex. Quantitative RT-PCR showed that expression of CCAP and the CCAP receptor displays a bimodal distribution, with peak mRNA levels in second instar larvae and pupae. Injection of CCAP revealed that augmenting hemocoelic CCAP levels in adult mosquitoes increases the anterograde and retrograde heart contraction rates by up to 28%, and increases intracardiac hemolymph flow velocities by up to 33%. Partial CCAP knockdown by RNAi had the opposite effect, decreasing the mosquito heart rate by 6%. Quantitative RT-PCR experiments showed that CCAP mRNA is enriched in the head region, and immunohistochemical experiments in newly eclosed mosquitoes detected CCAP in abdominal neurons and projections, some of which innervated the heart, but failed to detect CCAP in the abdomens of older mosquitoes. Instead, in older mosquitoes CCAP was detected in the pars lateralis, the subesophageal ganglion and the corpora cardiaca. In conclusion, CCAP has a potent effect on mosquito circulatory physiology, and thus heart physiology in this dipteran insect is under partial neuronal control. Supplementary material available online at

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Section: Discussionmentioning

confidence: 99%

Cardioacceleratory function of the neurohormone CCAP in the mosquito Anopheles gambiae

Estévez‐Lao

Boyce

Honegger

et al. 2013

Journal of Experimental Biology

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“…In D. melanogaster, immunoreactivity to receptors for AstA and DH31 has been found in the neurohemal release site of the corazonin neuroendocrine cells and these peptides may thus modulate corazonin release [41]. Furthermore, the AstA receptor DAR2 is expressed in insulin-producing neurons and the endocrine AKH cells in the corpora cardiaca [42].…”

Section: Functions Of Insect Epsmentioning

confidence: 99%

Chemical identity, function and regulation of enteroendocrine peptides in insects

Wegener

Veenstra

2015

Current Opinion in Insect Science

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“…But the biological functions of corazonin vary among different insects. Corazonin has been associated with polyphenism in migratory locusts, silk production in silkworms and ecdysis behavior in M. sexta (Veenstra 2009 ) . Corazonin receptor has been identi fi ed and functionally characterized in D. melanogaster , M. sexta and A. gambiae (Park et al 2002 ;Belmont et al 2006 ) .…”

Section: Neuropeptide Gpcrsmentioning

confidence: 99%