Does massage therapy immediately prior to embryo transfer improve clinical pregnancy rate in IVF-PGT-A (in-vitro fertilization-preimplantation genetic testing for aneuploidy) cycles?

Gavrizi, Sarah Z.; Skillern, A.

doi:10.1016/j.fertnstert.2019.07.598

Cited by 3 publications

(1 citation statement)

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“…Endometriosis (EM) is a benign gynecological disease that affects more than 10% of women of reproductive age ( Practice Committee of the American Society for Reproductive Medicine 2012 , Broi et al 2019 ). Infertility is observed in approximately 30–50% of EM patients ( Gavrizi & Skillern 2019 , Sanchez et al 2020 ). Despite the use of assisted reproductive technology therapy, patients with EM have a significantly lower clinical pregnancy rate compared to those without EM, and they also experience a higher rate of pregnancy-related complications ( Breintoft et al 2021 , Corachan et al 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Loss of KLF15 impairs endometrial receptivity by inhibiting EMT in endometriosis

Huang,

Wang,

et al. 2024

Journal of Endocrinology

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The impaired endometrial receptivity is a major factor contributing to infertility in patients with endometriosis (EM), but the underlying mechanism remains unclear. Our study aimed to investigate the role of Kruppel-like factor 15 (KLF15) in endometrial receptivity and its regulation in EM. We observed a significant decrease in KLF15 expression in the mid-secretory epithelial endometrial cells of EM patients compared to normal females without EM. To confirm the role of KLF15 in endometrial receptivity, we found a significantly reduced KLF15 expression and a significant decrease of embryo implantations number in the rat model via uterine horn infection with siRNA. This highlighted importance of KLF15 as a regulator receptivity. Furthermore, through ChIP-qPCR, we discovered that progesterone receptor (PR) directly binds to KLF15 promoter regions, indicating that progesterone resistance may mediate the decrease in KLF15 expression in EM patients. Additionally, we found that the mid-secretory endometrium of EM patients exhibited impaired epithelial-mesenchymal transition (EMT). Knockdown of KLF15 upregulated E-cadherin and downregulated Vimentin expression, leading to inhibited invasiveness and migration of Ishikawa cells. Overexpression KLF15 promotes EMT, invasiveness, and migration ability, and increased attachment rate of JAR cells to Ishikawa cells. Through RNA-seq analysis, we identified TWIST2 as a downstream gene of KLF15. We confirmed that KLF15 directly binds to the promoter region of TWIST2 via ChIP-qPCR, promoting epithelial cells EMT during the establishment of endometrial receptivity. Our study reveals the involvement of KLF15 in the regulation of endometrial receptivity and its downstream effects on EMT. These findings provide valuable insights into potential therapeutic approaches for treating non-receptive endometrium in patients with EM.

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Section: Introductionmentioning

confidence: 99%

Loss of KLF15 impairs endometrial receptivity by inhibiting EMT in endometriosis

Huang,

Wang,

et al. 2024

Journal of Endocrinology

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Abdominal massage: A review of clinical and experimental studies from 1990 to 2021

Wang¹,

Zhang²,

Sun³

et al. 2022

Complementary Therapies in Medicine

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Interventions to optimize embryo transfer in women undergoing assisted conception: a comprehensive systematic review and meta-analyses

Tyler

Walford

Tamblyn

et al. 2022

Human Reproduction Update

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BACKGROUND Several interventions and techniques are suggested to improve the outcome of embryo transfer (ET) in assisted conception. However, there remains no consensus on the optimal practice, with high variations among fertility specialists. OBJECTIVE AND RATIONALE We conducted a comprehensive systematic review and meta-analyses of randomized controlled trials (RCTs) aiming to identify effective interventions that could be introduced around the time of ET to improve reproductive outcomes. SEARCH METHODS We searched the electronic databases (MEDLINE, EMBASE and Cochrane CENTRAL) from inception until March 2021 using a multi-stage search strategy of MeSH terms and keywords, and included all RCTs that evaluated an intervention in the 24-h period before/after ET in women undergoing IVF/ICSI. Our primary outcome was clinical pregnancy rate post-ET confirmed as viable pregnancy on ultrasound scan. We assessed the risk of bias in included trials and extracted data in duplicate. We pooled data using a random-effect meta-analysis and reported using risk ratio (RR) with 95% CI. We explored publication bias and effect modifiers using subgroup analyses. OUTCOMES Our search yielded 3685 citations of which we included 188 RCTs (38 interventions, 59 530 participants) with a median sample size of 200 (range 26–1761). The quality of included RCTs was moderate with most showing a low risk of bias for randomization (118/188, 62.8%) and attrition (105/188, 55.8%) but there was a significant risk of publication bias (Egger’s test P = 0.001). Performing ET with ultrasound guidance versus clinical touch (n = 24, RR 1.265, 95% CI 1.151–1.391, I2 = 38.53%), hyaluronic acid versus routine care (n = 9, RR 1.457, 95% CI 1.197–1.261, I2 = 46.48%) and the use of a soft versus hard catheter (n = 27, RR 1.122, 95% CI 1.028–1.224, I2 = 57.66%) led to higher clinical pregnancy rates. Other pharmacological add-ons also showed a beneficial effect including granulocyte colony-stimulating factor (G-CSF: n = 4, RR 1.774, 95% CI 1.252–2.512, I2 = 0), Atosiban (n = 7, RR 1.493, 95% CI 1.184–1.882, I2 = 68.27%) and hCG (n = 17, RR 1.232, 95% CI 1.099–1.382, I2 = 57.76%). Bed rest following ET was associated with a reduction in clinical pregnancy (n = 6, RR 0.857, 95% CI 0.741–0.991, I2 = 0.01%). Other commonly used interventions, such as non-steroidal anti-inflammatory drugs, prophylactic antibiotics, acupuncture and cervical mucus removal, did not show a significant benefit on reproductive outcomes. Our effect estimates for other important outcomes, including miscarriage and live birth, were limited by the varied reporting across included RCTs. WIDER IMPLICATIONS Using ultrasound guidance, soft catheters and hyaluronic acid at the time of ET appears to increase clinical pregnancy rates. The use of Atosiban, G-CSF and hCG showed a trend towards increased clinical pregnancy rate, but larger trials are required before adopting these interventions in clinical practice. Bed rest post-ET was associated with a reduction in clinical pregnancy and should not be recommended.

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Does massage therapy immediately prior to embryo transfer improve clinical pregnancy rate in IVF-PGT-A (in-vitro fertilization-preimplantation genetic testing for aneuploidy) cycles?

Cited by 3 publications

References 0 publications

Loss of KLF15 impairs endometrial receptivity by inhibiting EMT in endometriosis

Loss of KLF15 impairs endometrial receptivity by inhibiting EMT in endometriosis

Abdominal massage: A review of clinical and experimental studies from 1990 to 2021

Interventions to optimize embryo transfer in women undergoing assisted conception: a comprehensive systematic review and meta-analyses

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