Does microcystin disrupt the induced effect of Daphnia kairomone on colony formation in Scenedesmus?

Combined effects of nitrogen levels and Daphnia culture filtrate on colony size of Scenedesmus obliquus

“…obliquus grew well under all nitrogen levels in this study. Two-way ANOVA results indicated that adding Daphnia culture filtrate did not affect the growth of S. obliquus; this result is consistent with that described in other studies [8,14,15]. Although nitrogen level significantly affected the growth rate of S. obliquus (Table 1), the differential degree was very low; in particular, growth rate only decreased by approximately 6% from 0.54 d −1 at 32 mg L − 1 N to 0.48 d − 1 at 2 mg L − 1 N (Fig.…”

“…2); this peak was evidently higher than that of the cultures with added Scenedesmus culture filtrate. This observation was in line with that described in other studies [6][7][8]14,15]. The effect of nitrogen level was also determined in this study.…”

“…The zooplankton grazer Daphnia magna was cultured in beakers and fed with S. obliquus (5 × 10 5 cells mL −1 ) under the same conditions as described above. Daphnia culture filtrate was then obtained as previously described [14,15]. In brief, D. magna cultures were collected, washed to remove surface residual medium, and allowed to fast in water for at least 12 h; afterward, D. magna cultures were incubated at a density of 200 ind L − 1 and fed with sufficient S. obliquus.…”

Waterborne copper impairs grazer-induced colony formation and photosynthetic efficiency inScenedesmus obliquus

“…Prior to the experiment, the filtrate of Daphnia ‐cultured medium ( Daphnia filtrate), which contained aliphatic sulfates and sulfamates (Yasumoto et al ), was prepared following standard previously described procedures (Lürling and Van Donk ; Lürling and Van Donk ; Zhu et al ). BG11 medium culturing S. obliquus that had not been exposed to Daphnia was filtered in the same way and used as the control filtrate.…”

Temperature-dependent effect of food size on the reproductive performances of the small-sized cladoceran Moina micrura

“…The two types of food size, namely, unicells and small colonies, were obtained by normal culturing or adding a small amount of the filtrate of Daphnia cultures (Lürling and Van Donk, 1996;Zhu et al, 2014), respectively. The combinations of treatment were 2 Â 3, and each treatment was performed in ten replicates.…”