Does perfluorooctane sulfonate (PFOS) act as chemosensitizer in zebrafish embryos?

Keiter, Susanne; Burkhardt-Medicke, Kathleen; Wellner, Peggy; Kais, Britta; Färber, Harald; Skutlarek, Dirk; Engwall, Magnus; Braunbeck, Thomas; Keiter, Steffen H.; Luckenbach, Till

doi:10.1016/j.scitotenv.2015.12.089

Cited by 27 publications

(12 citation statements)

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“…Given its wide distribution, perfluorooctane sulfonate (PFOS) has attracted most attention among perfluorinated substances, since it represents the main perfluorinated alkylated compounds detected in aquatic environments (Keiter et al, 2016). PFOS is an extremely persistent fluorinated pollutant that does not hydrolyze, photolyase or biodegrade.…”

mentioning

confidence: 99%

Multi-Laboratory Hazard Assessment of Contaminated Microplastic Particles by Means of Enhanced Fish Embryo Test With the Zebrafish (Danio rerio)

Cormier

Batel

Cachot

et al. 2019

Front. Environ. Sci.

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As wide-spread pollutants in the marine environment, microplastics (MPs) have raised public concern about potential toxic effects in aquatic organisms, and, among others, MPs were suspected to act as a vector for organic pollutants to biota. The purpose of the present study was to investigate effects by three model pollutants, oxybenzone (BP3), benzo[a]pyrene (BaP), and perfluorooctane sulfonate (PFOS) adsorbed to polyethylene MPs on the basis of a standard assay, the acute fish embryo toxicity test (FET; OECD TG 236) with zebrafish (Danio rerio) supplemented by additional endpoints such as induction of ethoxyresorufin-O-deethylase (EROD) activity, modification of cyp1a gene transcription and changes in larval swimming behavior. FET assays were performed in three laboratories using slightly different husbandry and exposure conditions, which, however, were all fully compatible with the limits defined by OECD TG 236. This allowed for testing of potential changes in the FET assay due to protocol variations. The standard endpoints of the FET (acute embryotoxicity) did not reveal any acute toxicity for both virgin MPs and MPs spiked with BP3, BaP, and PFOS. With respect to sublethal endpoints, EROD activity was increased after exposure to MPs spiked with BP3 (3 h pulse) and MPs spiked with BaP (96 h continuous exposure). Cyp1a transcription was increased upon exposure to MPs spiked with BP3 or BaP. For the selected combination of MPs particles and contaminants, the basic FET proved not sensitive enough to reveal effects of (virgin and spiked) MPs. However, given that the FET can easily be supplemented by a broad variety of more subtle and sensitive endpoints, an enhanced FET protocol may provide a relevant approach with developmental stages of a vertebrate animal model, which is not protected by current EU animal welfare legislation (Directive EU 2010/63).

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confidence: 99%

Multi-Laboratory Hazard Assessment of Contaminated Microplastic Particles by Means of Enhanced Fish Embryo Test With the Zebrafish (Danio rerio)

Cormier

Batel

Cachot

et al. 2019

Front. Environ. Sci.

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“…Changes were studied using expression analysis of genes involved in toxicity mechanisms of the investigated compounds. It was shown in previous studies that several PFASs such as PFOS are capable of altering membrane permeability (Hu et al, 2003;Keiter et al, 2016;Liao et al, 2014;Liu et al, 2008;Matyszewska and Bilewicz, 2008). Therefore, we hypothesized that the activation of the toxicity pathways of PCB126 would be increased by the presence of PFOS or/and PFHxA as a result of its higher cellular concentration.…”

Section: Discussionmentioning

confidence: 94%

“…Moreover, research has demonstrated that PFASs are able to modulate the toxicity of other chemicals (Jernbro et al, 2007) as "chemosensitizers". The exact process remains unclear; however, it was hypothesized that they alter biological membrane fluidity (Hu et al, 2003;Liao et al, 2014;Liu et al, 2008;Matyszewska and Bilewicz, 2008) and inhibit efflux transporter systems (Fischer et al, 2013;Keiter et al, 2016). Both effects may lead to increased local concentrations of xenobiotics in the cells and therefore may increase their toxicity.…”

Section: Introductionmentioning

confidence: 99%

“…Genes of the aryl hydrocarbon receptor (AhR) mediated pathway (ahr2, cyp1a) (Fang et al, 2012;Jonsson et al, 2007a;Jonsson et al, 2007b), the oxidative stress response (gpx1a) (Shi and Zhou, 2010), and a marker for apoptosis (tp53) (Shi et al, 2008) were studied. In order to address the possibility whether PFASs interfere with membrane integrity by altering lipid homeostasis, genes belonging to lipid metabolism (acaa2, osbpl1a) were monitored (Hu et al, 2005;Shi et al, 2009) Moreover, expression of two methyltransferases genes, dnmt1 and dnmt3ba, was measured in order to highlight underlying mechanisms of toxicity (Aluru et al, 2015 (Keiter et al, 2016): 25 mg/L (50µM) of PFOS, 15.7 mg/L (50 µM) of PFHxA and 7.5 µg/L (23 nM) of PCB126. Beakers used for exposures were pre-incubated for 24 hours with the chemical solutions in order to limit loss of compounds due to glass adsorption.…”

Section: Introductionmentioning

confidence: 99%

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Mixture-specific gene expression in zebrafish ( Danio rerio ) embryos exposed to perfluorooctane sulfonic acid (PFOS), perfluorohexanoic acid (PFHxA) and 3,3′,4,4′,5-pentachlorobiphenyl (PCB126)

Blanc

Kärrman

Kukučka

et al. 2017

Science of The Total Environment

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“…PFOS can alter the gene expression of the hypothalamic-pituitary-thyroid (HPT) axis of zebrafish and disrupt the synthesis and regulation of thyroid hormones (Shi et al 2009). PFOS can also suppress the activity of efflux transport proteins in zebrafish as a chemosensitizer (Keiter et al 2016).…”

Section: Speciesmentioning

confidence: 99%

A review on perfluoroalkyl acids studies: Environmental behaviors, toxic effects, and ecological and health risks

Liu

et al. 2019

Ecosyst Health Sustain

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Perfluoroalkyl acids (PFAAs) are emerging persistent organic pollutants that are globally distributed in the environment. In the present review, the occurrence of PFAAs and their behavior in aquatic ecosystem were summarized, and the health and ecological risk assessment and the multimedia fate simulation were investigated. PFAAs are most likely to exist in the aqueous phase, and PFAAs in atmosphere are also able to enter water bodies through diffusion and wet and dry deposition and eventually become widely distributed in various environmental media. The air-solid partition is considered to be one of the major factors in the long-distance transportation of the pollutants. The pKa values and organic carbon fraction of the sediment could influence the partition of PFAAs between water and sediment. Otherwise, PFAAs have teratogenic, mutagenic and other toxic effects and they could be accumulated by biota, and magnified through trophic level. The ecological and health risks of PFOA and PFOS were assessment. In order to explore the partition mechanism and reduce the uncertainty of the simulation of the transport, transformation and fate, the experimental methods on physicochemical properties of PFAAs should be developed. Moreover, further studies on toxicities of PFAAs are necessary for health and ecological risk assessment.

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Does perfluorooctane sulfonate (PFOS) act as chemosensitizer in zebrafish embryos?

Cited by 27 publications

References 50 publications

Multi-Laboratory Hazard Assessment of Contaminated Microplastic Particles by Means of Enhanced Fish Embryo Test With the Zebrafish (Danio rerio)

Multi-Laboratory Hazard Assessment of Contaminated Microplastic Particles by Means of Enhanced Fish Embryo Test With the Zebrafish (Danio rerio)

Mixture-specific gene expression in zebrafish ( Danio rerio ) embryos exposed to perfluorooctane sulfonic acid (PFOS), perfluorohexanoic acid (PFHxA) and 3,3′,4,4′,5-pentachlorobiphenyl (PCB126)

A review on perfluoroalkyl acids studies: Environmental behaviors, toxic effects, and ecological and health risks

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