Does size matter? A proteomics-informed comparison of the effects of polystyrene beads of different sizes on macrophages

Collin-Faure, Véronique; Dalzon, Bastien; Devcic, Julie; Diemer, Hélène; Cianférani, Sarah; Rabilloud, Thierry

doi:10.1039/d2en00214k

Cited by 6 publications

(7 citation statements)

References 95 publications

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“…The absence of measurable cytotoxicity does not mean that NPs have no effect on cells. A recent study [ 36 ] observed extensive proteome changes in the absence of the toxic effects of PS NPs on the J774A.1 cell line (mouse macrophages), which are mostly adaptive changes, and proposed that NPs may affect the fine functioning of the innate immune system and have indirect adverse effects. Therefore, although no significant difference in the PBMC response to unwashed NPs and washed NPs was observed in our study, it cannot be excluded that the presence of SDS induced a different set of adaptive changes in cell proteome, particularly in chronic exposure settings.…”

Section: Resultsmentioning

confidence: 99%

Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes

Djapovic,

Apostolovic,

Postic

et al. 2023

Polymers

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Manufactured nanoplastic particles (NPs) are indispensable for in vitro and in vivo testing and a health risk assessment of this emerging environmental contaminant is needed. The high surface area and inherent hydrophobicity of plastic materials makes the production of NPs devoid of any contaminants very challenging. In this study, we produced nanoprecipitated polyethylene terephthalate (PET) NPs (300 nm hydrodynamic diameter) with an overall yield of 0.76%. The presence of the ionic surfactant sodium dodecyl sulfate (SDS) was characterized by 1H NMR, where the relative ratio of NP/surfactant was monitored on the basis of the chemical shifts characteristic of PET and SDS. For a wide range of surfactant/NP ratios (17:100 to 1.2:100), the measured zeta potential changed from −42.10 to −34.93 mV, but with an NP concentration up to 100 μg/mL, no clear differences were observed in the cellular assays performed in protein-rich media on primary human cells. The remaining impurities contributed to the outcome of the biological assays applied in protein-free buffers, such as human red blood cell hemolysis. The presence of SDS increased the NP-induced hemolysis by 1.5% in protein-rich buffer and by 7.5% in protein-free buffer. As the size, shape, zeta potential, and contaminants of NPs may all be relevant parameters for the biological effects of NPs, the relative quantification of impurities exemplified in our work by the application of 1H NMR for PET NPs and the ionic surfactant SDS could be a valuable auxiliary method in the quality control of manufactured NPs.

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Section: Resultsmentioning

confidence: 99%

Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes

Djapovic,

Apostolovic,

Postic

et al. 2023

Polymers

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“…As we observed modulations in some cell surface molecules involved in molecular patterns recognitions (e.g. TLR7 or CD204) upon exposure to polystyrene beads through a proteomic screen (25), we investigated this phenomenon into more detail. The results, shown on Figure 9, indicated an increase in the TLR7 fluorescence for highly phagocytic cells, and this increase propagated to lesser phagocytic cells for large beads (>3µm).…”

Section: Pamps/dampsmentioning

confidence: 99%

“…These effects have been shown to be plastic concentrationdependent (22,23). More recently, results on the effects of polystyrene beads of different sizes on macrophages have suggested that some effects may be dependent on the amount of plastic internalized by the cells (25). We have therefore decided to investigate this concept further, and tried to determine if the cell population heterogeneity leads to different reactions to the exposure to micro or nanoplastics.…”

mentioning

confidence: 99%

The internal dose makes the poison: higher internalization of polystyrene particles induce increased perturbation of macrophages

Collin-Faure,

Vitipon,

Torres

et al. 2023

Front. Immunol.

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Plastics are emerging pollutants of great concern. Macroplastics released in the environment degrade into microplastics and nanoplastics. Because of their small size, these micro and nano plastic particles can enter the food chain and contaminate humans with still unknown biological effects. Plastics being particulate pollutants, they are handled in the human body by scavenger cells such as macrophages, which are important players in the innate immune system. Using polystyrene as a model of micro and nanoplastics, with size ranging from under 100 nm to 6 microns, we have showed that although non-toxic, polystyrene nano and microbeads alter the normal functioning of macrophages in a size and dose-dependent manner. Alterations in the oxidative stress, lysosomal and mitochondrial functions were detected, as well as changes in the expression of various surface markers involved in the immune response such as CD11a/b, CD18, CD86, PD-L1, or CD204. For each beads size tested, the alterations were more pronounced for the cell subpopulation that had internalized the highest number of beads. Across beads sizes, the alterations were more pronounced for beads in the supra-micron range than for beads in the sub-micron range. Overall, this means that internalization of high doses of polystyrene favors the emergence of subpopulations of macrophages with an altered phenotype, which may not only be less efficient in their functions but also alter the fine balance of the innate immune system.

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“…The modulated proteins could be classified majorly into: a) mitochondrial (NADH–ubiquinone oxidoreductase of respiratory complex I and ubiquinol–cytochrome c oxidoreductase of respiratory complex III), b) proteins involved in lipid metabolism, c) endo‐lysosomal (V‐type proton ATPases), d) structural/cytoskeletal (MICOS subunits Mic13 and Mic27, Sam50) and immune proteins (toll like receptors, CD antigens, integrin and their signaling proteins). [ 107 ]…”

Section: Pnps Alter the Protein Expression Patterns: Insights From Mo...mentioning

confidence: 99%

“…The modulated proteins could be classified majorly into: a) mitochondrial (NADH-ubiquinone oxidoreductase of respiratory complex I and ubiquinol-cytochrome c oxidoreductase of respiratory complex III), b) proteins involved in lipid metabolism, c) endo-lysosomal (V-type proton ATPases), d) structural/cytoskeletal (MICOS subunits Mic13 and Mic27, Sam50) and immune proteins (toll like receptors, CD antigens, integrin and their signaling proteins). [107] In environmental setup, PNPs do not remain as an individual polluting component and co-exposed to organisms with range of substances such as plasticizers, other environmental pollutants, industrial waste etc. Co-exposure of PNPs has been found to modulate the cellular interactome synergistically or antagonistically.…”

Section: Pnps Alter the Protein Expression Patterns: Insights From Mo...mentioning

confidence: 99%

Mechanistic Insights into Cellular and Molecular Basis of Protein‐Nanoplastic Interactions

Naidu,

Nagar,

Poluri

2023

Small

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Plastic waste is ubiquitously present across the world, and its nano/sub‐micron analogues (plastic nanoparticles, PNPs), raise severe environmental concerns affecting organisms’ health. Considering the direct and indirect toxic implications of PNPs, their biological impacts are actively being studied; lately, with special emphasis on cellular and molecular mechanistic intricacies. Combinatorial OMICS studies identified proteins as major regulators of PNP mediated cellular toxicity via activation of oxidative enzymes and generation of ROS. Alteration of protein function by PNPs results in DNA damage, organellar dysfunction, and autophagy, thus resulting in inflammation/cell death. The molecular mechanistic basis of these cellular toxic endeavors is fine‐tuned at the level of structural alterations in proteins of physiological relevance. Detailed biophysical studies on such protein‐PNP interactions evidenced prominent modifications in their structural architecture and conformational energy landscape. Another essential aspect of the protein‐PNP interactions includes bioenzymatic plastic degradation perspective, as the interactive units of plastics are essentially nano‐sized. Combining all these attributes of protein‐PNP interactions, the current review comprehensively documented the contemporary understanding of the concerned interactions in the light of cellular, molecular, kinetic/thermodynamic details. Additionally, the applicatory, economical facet of these interactions, PNP biogeochemical cycle and enzymatic advances pertaining to plastic degradation has also been discussed.

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Does size matter? A proteomics-informed comparison of the effects of polystyrene beads of different sizes on macrophages

Abstract: Macrophages treated with polystyrene beads of different sizes show different adaptive responses but no inflammatory responses.

Cited by 6 publications

References 95 publications

Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes

Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes

The internal dose makes the poison: higher internalization of polystyrene particles induce increased perturbation of macrophages

Mechanistic Insights into Cellular and Molecular Basis of Protein‐Nanoplastic Interactions

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