Does somaclonal variation play advantageous role in conservation practice of endangered species?: comprehensive genetic studies of in vitro propagated plantlets of Viola stagnina Kit. (Violaceae)

Żabicki, Piotr; Śliwińska, Elwira; Mitka, Józef; Sutkowska, Agnieszka; Tuleja, Monika; Migdałek, Grzegorz; Żabicka, Justyna; Słomka, Aneta; Kwiatkowska, Monika; Kuta, Elżbieta

doi:10.1007/s11240-018-1519-1

Cited by 23 publications

(18 citation statements)

References 61 publications

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“…This lack of genetic stability can be recognized as disadvantageous for conservation purposes. On the other hand, it is believed that it could be beneficial in the restitution of a small population [ 52 , 53 ]. Nevertheless, new genetic variation makes this propagation method a valuable source of new qualities potentially useful in horticulture.…”

Section: Discussionmentioning

confidence: 99%

Sourcing and Propagation of Pontechium maculatum for Horticulture and Species Restoration

Nowak

Sitek

Augustynowicz

2020

Biology

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Pontechium maculatum, a species of ornamental, apicultural, health and medicinal value, is threatened in some Central European countries including Poland. Its propagation using seeds or in vitro techniques is needed for multiple applications including conservation. Generative propagation efficacy of P. maculatum plants representing different genetic resources (received from botanical gardens in Germany and in Poland) propagated from seeds or in tissue culture was assessed. Moreover, an efficient technique of propagation of P. maculatum using in vitro shoot culture from seedlings was elaborated for the first time. The highest propagation efficacy was noted for German plants of seed origin. The ability of seeds to germinate was similar for all plants; however, seeds were in a state of dormancy, which was broken by GA3. After two years of storage, the seeds still retained the ability to germinate though seeds from propagated in vitro plants germinated more poorly than those from seed-originated plants. The ploidy assessment showed that some in vitro-origin plants had altered DNA content. The results indicate that efficacy of generative propagation of P. maculatum is resource dependent. Furthermore, results suggest that cultivation in vitro influenced some generative features of examined species, which makes this way of P. maculatum propagation a valuable source of genetic variation and a potential breeding tool.

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Section: Discussionmentioning

confidence: 99%

Sourcing and Propagation of Pontechium maculatum for Horticulture and Species Restoration

Nowak

Sitek

Augustynowicz

2020

Biology

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“…Depending on the genotype of plants and the type of explants, TDZ induces different regeneration responses (Passey et al, 2003). TDZ, with a high cytokinin-like activity (Huetteman and Preece, 1993), is widely used to promote shoot proliferation in many plant species, including the members of Violaceae (Sato et al, 1995;Slazak et al, 2015;Żabicki et al, 2019). The study by Slazak et al (2015) revealed that TDZ had the potential to induce direct and indirect organogenesis in leaf and petiole explants of Viola uliginosa Besser (Slazak et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, an efficient protocol is necessary for successful tissue culture and plant regeneration of Viola. In recent years there have been some effective reports on plant regeneration of the Viola species (Chalageri and Babu, 2012;Kaloo et al, 2013;Vishwakarma et al, 2013;Slazak et al, 2015;Żabicki et al, 2019), but there is no report on tissue culture and plant regeneration of V. caspia subsp. sylvestrioides.…”

Section: Introductionmentioning

confidence: 99%

In vitro regeneration and secondary metabolites of Viola caspia subsp. sylvestrioides Marcussen

Gharari¹,

Sharafi²,

Bagheri³

et al. 2019

bta

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Viola caspia subsp. sylvestrioides, a small important medicinal herb, belongs to the subsection Rostratae (sec. Viola) of genus Viola L. This study was performed to develop an efficient protocol for in vitro regeneration of V. caspia subsp. sylvestrioides as an ex situ technique for conservation of endangered plants and extraction of secondary metabolites. Leaf and petiole explants were cultured on half and full-strength Murashige and Skoog (MS) media supplemented with different concentrations and combinations of 6-benzylaminopurine (BAP), isopentenyl adenine (2ip), thidiazuron (TDZ), and naphthalene acetic acid (NAA). Direct organogenesis was induced on half and full-strength MS media supplemented with TDZ (0.7 to 3.5 mg/l) from leaf and petiole explants. Indirect shoot organogenesis was induced on half-strength MS supplemented with 3.5 mg/l 2ip and 2.5 mg/l NAA followed by transferring the obtained callus onto a half-strength MS containing 0.5 mg/l BAP. The highest frequency of shoot organogenesis (100 and 86.66%) was noted for petiole and leaf explants, respectively, on halfstrength MS medium supplemented with 2.8 mg/l TDZ (approx. 7.66 and 4.33 shoots per petiole and leaf explant, respectively). The induced shoots were used for root induction on a half-strength MS medium with 0.5 mg/l indole-3-butyric acid (IBA). Phytochemical constituents in leaf tincture were determined by gas chromatography/mass spectrometry (GC/MS) analysis. GC/MS results revealed that vitamin E was the main component in the tincture of Viola leaves. The present study provides a simple and rapid protocol for in vitro regeneration of V. caspia subsp. sylvestrioides plants which can be used for gene transfer and conservation purposes, and in pharmaceutical studies.

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“…However, it should be kept in mind that it can stimulate genetic variability (Us-Camas et al, 2014), and thus, plants obtained through micropropagation should be carefully evaluated. Presently, this evaluation involves an array of molecular techniques (Moharana et al, 2018;Nalousi et al, 2019;Sharma et al, 2019); however, a complete comparison should also include ploidy assessment and qualitative features (Jędrzejczyk et al, 2018;Nalousi et al, 2019;Żabicki et al, 2019). Molecularly confirmed genetic fidelity only concerns fragments of the genome, not necessarily associated with trait inheritance.…”

Section: In Vitro Propagationmentioning

confidence: 99%

“…Effects of environmental conditions during seed production on seed dormancy and germination have already been described. Low temperature before seed maturation may interfere with ABA biosynthesis or signaling and can markedly increase the final depth of seed dormancy (Chahtane et al, 2017;Chen et al, 2014;Kar et al, 2019;Sharma et al, 2019;Trejgell et al, 2012;Żabicki et al, 2019). However, numerous studies have shown that seeds maturing at higher temperatures produce more impermeable seeds when compared with those growing in a low-temperature environment (Jaganathan, 2016).…”

Section: Comparison Of Seed-derived and Micropropagated Plantsmentioning

confidence: 99%

Application of Horticultural and Tissue Culture Methods for Ex Situ Conservation of Endangered Primula farinosa L.

et al. 2020

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Our study aimed at active conservation of the last location of Primula farinosa, an endangered species in Poland, and assessed reproduction by seeds and plant propagation on sterile media in tissue culture conditions. We identified gibberellic acid (GA3) as the key factor stimulating germination of P. farinosa seeds. Growing juvenile plants under controlled temperature of 18/16 °C day/night yielded good quality plant material without mycorrhization. In tissue culture, the most favorable medium for shoot propagation was MS supplemented with the lowest tested concentration of indole-3-butyric acid (IBA; 0.05 mg dm−3) and 6-benzyl-aminopurine (BAP; 0.1 mg dm−3). The rooting ability of shoots was high and comparable for all auxins used. 2C DNA content of seed-derived and micropropagated plants did not indicate any change in the ploidy level during in vitro cultivation. Plants derived from seeds and tissue cultures were compared in a 2-year study. Of all the characteristics compared, only the number of flowers per inflorescence was lower for micropropagated plants when compared with the seed-origin plants in the first year of observation. The difference was of transient nature and was not observed in the second year of the study. Effective protocols for in vivo and in vitro propagation of P. farinosa were developed, which can be used in practical species protection.

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Does somaclonal variation play advantageous role in conservation practice of endangered species?: comprehensive genetic studies of in vitro propagated plantlets of Viola stagnina Kit. (Violaceae)

Cited by 23 publications

References 61 publications

Sourcing and Propagation of Pontechium maculatum for Horticulture and Species Restoration

Sourcing and Propagation of Pontechium maculatum for Horticulture and Species Restoration

In vitro regeneration and secondary metabolites of Viola caspia subsp. sylvestrioides Marcussen

Application of Horticultural and Tissue Culture Methods for Ex Situ Conservation of Endangered Primula farinosa L.

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