Exploring the green transition of cultivated land use from the perspective of green utilization efficiency evaluation has become an important content of deepening the study of cultivated land use transition, which is of great significance to promote food security and ecological civilization construction. At present, there are few studies on the green utilization efficiency of cultivated land (GUECL), which covers the comprehensive benefits of economy, ecology and society, combined with the requirements of ecological civilization and green development. Taking 65 cities (regions and autonomous prefectures) of the Yellow River Basin as the basic evaluation unit, the GUECL of the Yellow River Basin is evaluated with a Super-SBM model. In general, the GUECL of the Yellow River Basin was not high at four time points of 2000, 2006, 2012 and 2018, which presents a trend of “rising first and then falling”. Analyzing its temporal and spatial evolution pattern, the GUECL in the upper, middle and lower reaches presented an order of the upper reaches area > the lower reaches area > the middle reaches area; and the spatial variation trend showed a decrease from west to east, and a U-shaped change in the south-north direction. Using spatial correlation analysis, except for the year 2000, the GUECL in the Yellow River Basin presents a general distribution characteristic of spatial agglomeration, which is positively correlated in 2006, 2012 and 2018. The change of spatio-temporal pattern is the result of internal and external factors. The former mainly displays in the main characteristics of farmers, family characteristics and farmers’ cognition, while the latter is reflected in natural, social and policy factors.