Does the Novel Class of (2R, 4S)-N-(2, 5-Difluorophenyl)-4-Hydroxy-1-(2, 2, 2-Trifluoroacetyl) Pyrrolidine-2-Carboxamide’s Have Any Effect on Cell Viability and Apoptosis of Human Hepatocellular Carcinoma Cells?

Ramezani, Mahnaz; Ramezani, Mohammad; Hassanshahi, Gholamhossein; Mahmoodi, Mehdi; Zainodini, Nahid; Darekordi, Ali; Falahati-Pour, Soudeh Khanamani; Mirzaei, Peiman

doi:10.5812/ijcm.8413

Cited by 10 publications

(7 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As a result, it is urgently required that new therapeutic modalities be introduced for the gastric tumor. The major sources of novel therapeutic molecules against cancers are reported to be natural products (Zainodini et al, 2018) or chemically synthesized such as, metallodrugs (Aliabad et al, 2018;Mohammadizadeh et al, 2018;Rezaei et al, 2018), proline-derived compounds (Ramezani et al, 2017) and etc. Herbal materials are increasingly examined aimed at finding new anti-proliferative/ cytostatic compounds which are active.…”

Section: Discussionmentioning

confidence: 99%

Evaluating of Induction of Apoptosis by Cornus mass L. Extract in the Gastric Carcinoma Cell Line (AGS)

Hosseini

Karimabad

Hajizadeh

et al. 2019

Asian Pac J Cancer Prev

Self Cite

View full text Add to dashboard Cite

Aim and objectives: Natural products and derivatives of medicinal vegetation can play an important role to the cure tumor. The Present study was focused to determine the effect of Cornus mass L. extract on the induction of apoptosis in AGS gastric carcinoma cell line in compared to L929 cells. Methods: In this experimental study, AGS and L929 cells were cultured and treated with different concentrations (0-10 mg/ml) of Cornus mass L. extract for 48 and 72 hours. Cell proliferation was assessed by MTT assay. The optical density of the colored solution was quantified at 570 nm wavelengths by an ELISA Reader. Making use of the apoptosis detection kit of Annexin V-FITC, PI and double staining with Annexin V-FITC were carried out for flow cytometry investigations. Data were analyzed by ANOVA. Variations with a P-value less than 0.05 were considered significant. Results: shows a noticeable deviation among various concentrations of extract when cells were treated for 48, 72 h declined cell viability in AGS cell line in comparison L929 cell lines in a dose and time-dependent manner (P < 0.05). This extract also displayed approximately several-fold increased anti-cancer potency in AGS compared to L929 cells. The IC 50 value in AGS cells (evaluated after 48,72h) of the extract against AGS cells was 5/44, 2/44 mg/ml (p≤0.05). The analysis results of flow cytometry indicated that apoptosis was induced by the extract in AGS cells treated, compared with L929 cells. Conclusion: Each of our results implicates the reality that Cornus mass L. extract acts as a novel, potent inhibitor of cancer proliferation in in vitro. This may result in developing a promising therapeutic agent for the treatment of indole-sensitive cancers.

show abstract

Section: Discussionmentioning

confidence: 99%

Evaluating of Induction of Apoptosis by Cornus mass L. Extract in the Gastric Carcinoma Cell Line (AGS)

Hosseini

Karimabad

Hajizadeh

et al. 2019

Asian Pac J Cancer Prev

Self Cite

View full text Add to dashboard Cite

show abstract

“…The present study examined the apoptotic effects of pyrrolidine derivatives on human hepatoma (HepG2) cells. In a similar study, the cytotoxic effects of this derivative were explored on HepG2 cells by the MTT assay, and the cells were double-stained with Annexin-V and PI for the in-vitro flow cytometric detection of apoptotic cells, with the results indicating that it induced HepG2 cell apoptosis significantly at 125µL concentrations after 48 hours (Ramezani et al, 2017). To achieve the research goals, the authors of this article extended the mentioned study making use of the PCR array technology.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, to explore the underlying molecular mechanisms of apoptosis induced by the pyrrolidine derivative, the PCR array technology was used in this article. Research results indicate that pyrrolidine is able to modify apoptosis-induced gene expression (Ramezani et al, 2017). To better understand molecular mechanisms, we specified the expression of apoptosis-related genes by a quantitative real-time PCR array technology and found out that this derivative reduced cell viability and induced apoptosis.…”

Section: Discussionmentioning

confidence: 99%

“…Next, they were supplemented with 10% fetal bovine serum (FBS) (Gibco, USA), penicillin-streptomycin (100 U/mL) and incubated at 37°C in a humidified atmosphere containing 95% O 2 and 5% CO 2 . As already mentioned in brief, cell growth inhibition (IC 50 ) was calculated to be 50% for pyrrolidine-2-carboxamide after 48 hours (62.5µg/mL) (Ramezani et al, 2017). The nonmalignant cells (L929) were also cultured under similar conditions in the RPMI 1640 medium containing 5% (v/v) of fetal bovine serum (FBS) and 100 units/mL of penicillin-streptomycin.…”

Section: The Cell Culture Methodsmentioning

confidence: 99%

“…Next, the produced stock solution was filtered through a 0.45µM filter, before use in each assay. In the following step, 400µL of the stock solution was mixed up gently and enriched with 600µL of RPMI 1640 at appropriate concentrations (125µg), which was required for examinations by serial dilution (Ramezani et al, 2017).…”

Section: Chemical Compound Preparationmentioning

confidence: 99%

See 2 more Smart Citations

The New Compound of (2R, 4S)-N-(2, 5-difluorophenyl)-4- Hydroxy-1-(2, 2, 2-Trifluoroacetyl) Pyrrolidine-2-Carboxamide to Mediate the Expression of Some Apoptosis Genes by the HepG2 Cell Line

Ramezani

Darehkordi

et al. 2019

Asian Pac J Cancer Prev

Self Cite

View full text Add to dashboard Cite

Objectives:Hepatocellular carcinoma is one of the most frequent cancers worldwide, for the treatment of which various therapy protocols and drugs have been introduced; however, none of them has suppressed cancer tissues completely. New research programs have been developed on cancer and the accompanied effects of novel synthesized compounds on cancer cell lines. Our latest reports on the molecular basis of cancer revealed a pattern of changes in gene expression triggered in the cancer pathway.Methods:HepG2 cell lines were cultured under similar conditions in both test and control groups. The IC50 concentration of the (2R, 4S)-N-(2, 5-difluorophenyl)-4-hydroxy-1-(2, 2, 2-trifluoroacetyl) pyrrolidine-2-carboxamide compound was used in the treatment group. After 48 hours from the culture, the expressional profiles of apoptosis pathway genes (84 genes) were studied using the PCR array method.Results:The findings demonstrated that the expression of some apoptosis-related genes pertaining to TNF, BCL2, IAP, and caspase families was regulated by (2R, 4S)-N-(2, 5-difluorophenyl)-4-Hydroxy-1-(2, 2, 2-Trifluoroacetyl) Pyrrolidine-2-Carboxamide. In the same vein, an alteration was observed in the expression of both pro-apoptotic and anti-apoptotic genes associated with the extrinsic and intrinsic apoptosis signaling pathways.Conclusions:According to the data obtained, the pyrrolidine-2-carboxamide compound was demonstrated to be able to regulate the apoptotic activities of HepG2 cells by affecting both pro-apoptotic and anti-apoptotic relevant genes.

show abstract

A novel copper (II) complex activated both extrinsic and intrinsic apoptotic pathways in liver cancerous cells

Rezaei

Mahmoodi

Mohammadizadeh

et al. 2019

J of Cellular Biochemistry

Self Cite

View full text Add to dashboard Cite

Recent advances have put fundamental focus on the application of copper (II) (Cu [II]) complexes as agents for fighting against cancer. To determine whether [Cu(L)(2imi)] complex as a novel Cu complex can induce apoptosis in HepG2 as cancerous cells and L929 as normal cells via extrinsic or intrinsic apoptotic pathways, both cell lines were treated for 24 and 48 hours at IC 50 concentrations of [Cu(L)(2imi)] complex. Then, the expression of some apoptosis-related genes including p53, caspase-8, bcl-2, and bax were assayed by real-time polymerase chain reaction. The [Cu(L)(2imi)] complex seems to inhibit the expression of bcl-2 in complex-treated HepG2 cancerous cells following the 24-and 48-hour treatment. The complex upregulated the p53, bax, and caspase-8 genes, therefore treatment of HepG2 cancerous cells with [Cu(L)(2imi)] complex induces programmed cell death via the upregulation of relative bax/bcl-2 ratio. Finally, this copper complex triggered apoptosis in HepG2 cells via both intrinsic and extrinsic pathway, whereas treatment of normal L929 cells with this complex induce apoptosis only via intrinsic pathway with the upregulation of relative bax/bcl-2 ratio and does not affect the expression level of caspase-8 gene and does not trigger the extrinsic pathway. Finally, these results obtained from present study confirm the role of a novel Cu complex on the induction of apoptosis process in HepG2 and L929 cells by overexpression of bax, inhibition of bcl-2 and increase of the relative bax/bcl-2 ratio. These results support that the [Cu(L)(2imi)] complex is able to induce apoptosis in cancerous cells, therefore, it has a potential for development as a novel anticancer drug. K E Y W O R D S bax, bcl-2, caspase-8, [Cu(L)(2imi)] complex, hepatocellular carcinoma, p53

show abstract

Does the Novel Class of (2R, 4S)-N-(2, 5-Difluorophenyl)-4-Hydroxy-1-(2, 2, 2-Trifluoroacetyl) Pyrrolidine-2-Carboxamide’s Have Any Effect on Cell Viability and Apoptosis of Human Hepatocellular Carcinoma Cells?

Cited by 10 publications

References 16 publications

Evaluating of Induction of Apoptosis by Cornus mass L. Extract in the Gastric Carcinoma Cell Line (AGS)

Evaluating of Induction of Apoptosis by Cornus mass L. Extract in the Gastric Carcinoma Cell Line (AGS)

The New Compound of (2R, 4S)-N-(2, 5-difluorophenyl)-4- Hydroxy-1-(2, 2, 2-Trifluoroacetyl) Pyrrolidine-2-Carboxamide to Mediate the Expression of Some Apoptosis Genes by the HepG2 Cell Line

A novel copper (II) complex activated both extrinsic and intrinsic apoptotic pathways in liver cancerous cells

Contact Info

Product

Resources

About