Dog mitochondrial genome sequencing to enhance dog mtDNA discrimination power in forensic casework

Verscheure, Sophie; Backeljau, Thierry; Desmyter, Stijn

doi:10.1016/j.fsigen.2014.05.001

Cited by 19 publications

(16 citation statements)

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“…The LH ratio was calculated by dividing the signal intensity of the minor peak visible below the LH marker of the major haplotype by the sum of the signal intensities of the major and minor haplotype peaks at that position (Online Resource 2). The LH ratio and ma/mi haplotype combination was established for all pCpTpC stretch sequences generated in this study and those of the 214 dogs from a previous Belgian population study [36,44].…”

Section: Discussionmentioning

confidence: 99%

“…The dogs were selected from a Belgian population sample of 214 dogs [36,44] (specimens 16,22,24,30,36,37,50,52,61,68,75,79,83,106,110,117, and 128 in Suppl. PCR amplification and sequencing of the pCpTpC stretch Amplification of the pCpTpC stretch of DNA extracted from buccal swabs and hair shafts, with Novagen® Dog Genomic DNA (Merck KGaA, Darmstadt, Germany) as a positive control, was performed using primer pair cL16546-cH00036 ( Table 1).…”

Section: Specimensmentioning

confidence: 99%

“…Sequencing was performed using the amplification primers. The sequencing reactions, subsequent product purification, and capillary electrophoresis were performed according to the mitochondrial Primer names start with cL or cH denoting a forward or reverse primer, respectively; the numbers refer to the 3′ position of the primer binding site relative to the Kim et al [35] reference sequence genome sequencing protocol by Verscheure et al [44]. .…”

Section: Specimensmentioning

confidence: 99%

“…The goal of the present study was threefold: (1) to determine the range of pCpTpC haplotypes that exist in a Belgian population sample of 214 dogs [36,44], (2) to assess whether pCpTpC haplotypes differed intraindividually between DNA from buccal cells and hairs, and (3) to verify whether in vitro polymerase errors contribute to the LH. The impact of the findings on the forensic relevance and the manner of difference-coded annotation of the pCpTpC stretch was also discussed.…”

Section: Introductionmentioning

confidence: 99%

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Length heteroplasmy of the polyC-polyT-polyC stretch in the dog mtDNA control region

2014

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Previously, the mitochondrial control region of 214 Belgian dogs was sequenced. Analysis of this data indicated length heteroplasmy of the polyT stretch in the polyC-polyT-polyC stretch from positions 16661 to 16674. Nine polyC-polyT-polyC haplotype combinations were observed, consisting of seven major haplotypes (highest signal intensity) combined with minor haplotypes (lower signal intensity) one T shorter than the major haplotype in all but three dogs. The longer the polyT stretch, the smaller was the difference in signal intensity between the major and minor haplotype peaks. Additional sequencing, cloning, and PCR trap experiments were performed to further study the intra-individual variation of this mitochondrial DNA (mtDNA) region. Cloning experiments demonstrated that the proportion of clones displaying the minor haplotypes also increased with the length of the polyT stretch. Clone amplification showed that in vitro polymerase errors might contribute to the length heteroplasmy of polyT stretches with at least 10 Ts. Although major and minor polyC-polyT-polyC haplotypes did not differ intra-individually within and between tissues in this study, interpretation of polyT stretch variation should be handled with care in forensic casework.

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Section: Discussionmentioning

confidence: 99%

Section: Specimensmentioning

confidence: 99%

Section: Specimensmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Length heteroplasmy of the polyC-polyT-polyC stretch in the dog mtDNA control region

2014

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“…The STR marker-sets which have been developed have relevant population studies, occasionally extended with genetic variance [258][259][260][261][262][263][264], inbreeding [258,261] and mutation data [265]. Sequence databases of canine mitochondrial DNA have also been developed [266][267][268].…”

Section: Dna Analysis Of Nonhuman Remainsmentioning

confidence: 99%

Forensic DNA Technological Advancements as an Emerging Perspective on Medico-Legal Autopsy: A Mini Review

Pádár¹,

Zenke²,

Kozma³

2018

Post Mortem Examination and Autopsy - Current Issues From Death to Laboratory Analysis

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The importance of biological traces and evidences related to a criminal matter has been recognized for a long time. The examination of the expression of genetic polymorphism has been an integral part of the multidisciplinary field of medico-legal autopsy for over a century. Since the initial application of blood group antigens for personalization of a putative perpetrator in a murder case, the discipline of forensic genetics has evolved as a standard of forensic sciences. The real breakthrough, the application of molecular tools and processes for the in-vitro replication of genetic substances, has increasingly allowed the exploitation of advances of molecular genetics for both forensic and criminal investigations. Although there are certainly many more applications and scientific fields in the medico-legal arena, the relatively fast progress of genetics, which has accelerated recently with state-of-art technologies, can provide ever more relevant information in relation to a corpse or the cause and manner that resulted in the corpse for autopsy. This topic concerns the currently accepted forensic DNA technology, and the last section reviews commonly used markers for nuclear and mitochondrial DNA analysis as well as ongoing research. This review also focuses on the increasingly important non-human sources of DNA, and shortly covers the main aspects of animal forensic DNA examination.

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Forensic Hair Microscopy

Beckert

2020

Handbook of Trace Evidence Analysis

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Dog mitochondrial genome sequencing to enhance dog mtDNA discrimination power in forensic casework

Cited by 19 publications

References 50 publications

Length heteroplasmy of the polyC-polyT-polyC stretch in the dog mtDNA control region

Length heteroplasmy of the polyC-polyT-polyC stretch in the dog mtDNA control region

Forensic DNA Technological Advancements as an Emerging Perspective on Medico-Legal Autopsy: A Mini Review

Forensic Hair Microscopy

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