Domain redistribution within ergosterol-containing model membranes in the presence of the antimicrobial compound fengycin

Mantil, Elisabeth; Crippin, Trinda; Avis, Tyler J.

doi:10.1016/j.bbamem.2019.01.003

Cited by 17 publications

(3 citation statements)

References 52 publications

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“…Morpho-structural alterations of the cell surface and membrane perturbations of several fungal pathogens have been attributed to the effect of CLP (Horn et al, 2013;Wise et al, 2014;Sur et al, 2018;Mantil et al, 2019). Although antifungal activity of CLP has been widely reported (Cawoy et al, 2015), there is less evidence about the effect of these antimicrobials on bacteria.…”

Section: Discussionmentioning

confidence: 99%

Fengycins From Bacillus amyloliquefaciens MEP218 Exhibit Antibacterial Activity by Producing Alterations on the Cell Surface of the Pathogens Xanthomonas axonopodis pv. vesicatoria and Pseudomonas aeruginosa PA01

Medeot

Fernandez²,

Morales³

et al. 2020

Front. Microbiol.

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Section: Discussionmentioning

confidence: 99%

Fengycins From Bacillus amyloliquefaciens MEP218 Exhibit Antibacterial Activity by Producing Alterations on the Cell Surface of the Pathogens Xanthomonas axonopodis pv. vesicatoria and Pseudomonas aeruginosa PA01

Medeot

Fernandez²,

Morales³

et al. 2020

Front. Microbiol.

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“…Fluid state of membrane lipids may also be key for effective CLPs insertion (Deleu et al, 2008; Mantil et al, 2019). In agreement, more recent studies, on model membrane, showed that bilayers containing higher levels of ergosterol exhibited an increased tolerance to activity of fengycin at low doses (Mantil et al, 2019). All these information could explain the differences measured in lipopeptide antifungal activity between tebuconazole sensitive and reduced sensitivity strains of V. inaequalis .…”

Section: Discussionmentioning

confidence: 99%

Antifungal Activities of Bacillus subtilis Lipopeptides to Two Venturia inaequalis Strains Possessing Different Tebuconazole Sensitivity

et al. 2019

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Within the framework of biocontrol development, three natural substances produced by Bacillus subtilis, called lipopeptides, have been studied: fengycin (F), surfactin (S), and mycosubtilin (M). Their antifungal properties were tested in vitro, in liquid medium, on two strains of Venturia inaequalis, ascomycete fungi causing apple scab. These two strains were, respectively sensitive and less sensitive to tebuconazole, an active substance of the triazole family. These three molecules were tested on their own, in binary (FS, FM, SM) and ternary mixtures (FSM). The antifungal activities of lipopeptides were estimated by calculating an IC50, compared to tebuconazole chemical substance. In tests involving the sensitive strain, all lipopeptide modalities exhibited antifungal activity. However, modalities involving fengycin and its mixtures exhibited the best antifungal activities; the activity of fengycin alone being very similar to that of tebuconazole. Interestingly, regarding the strain with reduced sensitivity to tebuconazole, surfactin and fengycin alone were not efficient while mycosubtilin and the different mixtures showed interesting antifungal activities. Specifically, the antifungal activity of FS and FSM mixture were equivalent to that of tebuconazole. For both fungal strains, microscopic observations revealed important morphological modifications in the presence of fengycin and in a less important proportion in the presence of surfactin but not in the presence of mycosubtilin. Overall, this study highlights the diversity in mode of action of lipopeptides on apple scab strains.

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“…The present work focused on the affects of the presence or absence of cholesterol, but going forward with ergosterol is the next logical step, especially the recent work showing that ergosterol also modulates fengycin function. (49,50) However, in the context of the MARTINI model cholesterol and ergosterol are very similar, so much so that distinguishing their effects appeared unlikely to succeed. We suspect that repeating these calculations with fully atomistic methods will be necessary.…”

Section: Discussionmentioning

confidence: 99%

Effects of Cholesterol on the mechanism of fengycin, a biofungicide

Sur

Grossfield

2021

Preprint

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Fengycins are a class of antifungal lipopeptides synthesized by the bacteria Bacillus subtilis, commercially available as the primary component of the agricultural fungicide Serenade. They are toxic to fungi, but far less to mammalian cells. One key difference between mammalian and fungal cell membranes is the presence of cholesterol only in the former; recent experimental work showed that the presence of cholesterol reduces fengycin-induced membrane leakage. Since our previous all-atom and coarse-grained simulations suggested that aggregation of membrane-bound fengycin is central to its ability to disrupt membranes, we hypothesized that cholesterol might reduce fengycin aggregation. Here, we test this hypothesis using coarse-grained molecular dynamics simulations, with sampling enhanced via the weighted ensemble method. The results indicate that cholesterol subtly alters the size distribution for fengycin aggregates, limits the lateral range of their membrane disordering, and reduces the ability of aggregates to bend the membrane. Taken together, these phenomena may account for cholesterols' affects on fengycin activity.

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Domain redistribution within ergosterol-containing model membranes in the presence of the antimicrobial compound fengycin

Cited by 17 publications

References 52 publications

Fengycins From Bacillus amyloliquefaciens MEP218 Exhibit Antibacterial Activity by Producing Alterations on the Cell Surface of the Pathogens Xanthomonas axonopodis pv. vesicatoria and Pseudomonas aeruginosa PA01

Fengycins From Bacillus amyloliquefaciens MEP218 Exhibit Antibacterial Activity by Producing Alterations on the Cell Surface of the Pathogens Xanthomonas axonopodis pv. vesicatoria and Pseudomonas aeruginosa PA01

Antifungal Activities of Bacillus subtilis Lipopeptides to Two Venturia inaequalis Strains Possessing Different Tebuconazole Sensitivity

Effects of Cholesterol on the mechanism of fengycin, a biofungicide

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