Domibacillus mangrovi sp. nov. and Domibacillus epiphyticus sp. nov., isolated from marine habitats of the central west coast of India

Abstract

While studying culturable bacterial diversity in different marine habitats of the central west coast of India, two novel Gram-stain-positive, strictly aerobic, motile, endospore-forming and rod-shaped bacterial strains designated as SAOS 44T and SAB 38T were isolated from mangrove sediment soil and the surface of a macroalga, respectively. The strains were taxonomically characterized by using a polyphasic approach and genomic methods. The phylogenetic analysis based on 16S rRNA gene sequencing placed the strains firmly in the genus Domibacillus and were most closely related to Domibacillus antri KCTC 33636T. The predominant fatty acids were iso-C15 : 0 and anteiso-C15 : 0. The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol in addition to an aminophosphoglycolipid. MK-6 was the only respiratory quinone. The range of values of digital DNA-DNA hybridization (19.2-24.9 %) and the ortho-average nucleotide identity (74.1-81.4 %) among strains SAOS 44T, SAB 38T and other Domibacillus species clearly supports their status as a distinct and novel species for which the names Domibacillusmangrovi sp. nov. SAOS 44T (=DSM 100930T=KCTC 33820T=MTCC 12571T) and Domibacillus epiphyticus sp. nov. SAB 38T (=DSM 100929T=KCTC 33830T=MTCC 12575T) are proposed, respectively.

Microbial composition in seasonal time series of free tropospheric air and precipitation reveals community separation

“…? oxidation , Domibacillus (Firmicutes), a gram-positive, spore-forming, aerobic, nonmotile, rod-shaped organism isolated from deep-sea sediment (Sun and Sun 2016), ocean sediment (Sharma et al 2014), mangrove sediment soil and macroalgae (Verma et al 2017) and GP1 (Acidobacteria), found in alpine grassland on the Tibetian Plateau (Yuan et al 2014). Winter samples were more characterized by extremophile but omnipresent bacteria like Bifidobacterium (Actinobacteria) that can withstand a high range of pH (from 8 to 2) (Charteris et al 1998), Vulcaniibacterium, a thermotolerant Gammaproteobacteria isolated from a geothermally heated soil (Yu et al 2013), and Polaribacter (Bacteriodetes), which has been found in several arctic habitats (Cuthbertson et al 2017;Harding et al 2011;Collins et al 2010).…”

Microbial communities on UV cabins in the dermatology service of a Spanish hospital: ecology and resistance to UV light and antibiotics

“…Apart from the above-described ones, there is also a less abundant representation of some environmental-associated taxa, many of which have been isolated from varied environments such as Deinococcus, Domibacillus, Pantoea or Sphingomonas [57][58][59][60]. Others have been linked to isolated cases of fungaemia, bacteremia or sepsis, such as Aureobasidium, Kosakonia, Lysinibacillus or Massilia [61-64].…”

Halomonas icarae sp. nov., a moderately halophilic bacterium isolated from beach soil in India

“…For chemotaxonomic analysis, freeze-dried cells were prepared by growing in TSB+ASW for 2 days at 30 °C. The respiratory quinone of strain D1-1 T and its closely related taxa were extracted using the procedure described by Verma et al [33]. Briefly, 100 mg freeze-dried cells were incubated with 2 ml aqueous methanol and 2 ml light petrol (BP; 60-80 °C) in Teflon-lined screw cap tubes.…”