Donor tissue and culture condition effects on mesophyll protoplasts of Medicago sativa

Dijak, M.; Brown, Daniel C.

doi:10.1007/bf00040807

Cited by 5 publications

(2 citation statements)

References 23 publications

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“…red-enriched light radiations, low phosphorus and low calcium concentrations, influenced subsequent development of the protoplasts. In the same way the isolation procedures and protoplasts pretreatment modified the protoplasts embryogenic capacity [6,7].…”

Section: Introductionmentioning

confidence: 99%

Plant donor tissue and isolation procedure effect on early formation of embryoids from protoplasts of Helianthus annuus L.

Dupuis¹,

Péan²,

Chagvardieff

1990

Plant Cell Tiss Organ Cult

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The effects of factors influencing sunflower protoplast isolation yield, plating efficiency (PE) and the early differentiation into embryoids (embryogenic capacity, EC) have been studied. Only hypocotyl-derived protoplasts divided. The variations of PE and EC in the various treatments did not seem to be linked to the protoplast yields. From statistical analysis of the data, we concluded that, the sunflower genotype, the age and height of seedlings, the part of hypocotyl used, the incubation time (from 6 to 16 hours) in enzymes of explants or of protoplasts alone, influenced PE but large variations were detected for EC. A comparison of the factors effecting EC suggested an origin, inside the hypocotyl, of cells able to give rise, after induction, to embryogenic protoplasts.

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Section: Introductionmentioning

confidence: 99%

Plant donor tissue and isolation procedure effect on early formation of embryoids from protoplasts of Helianthus annuus L.

Dupuis¹,

Péan²,

Chagvardieff

1990

Plant Cell Tiss Organ Cult

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“…For example, dipping petunia leaf segments, leaves or other explants in cytokinin solutions such as benzyl adenine prior to culture (Read et al, 1978;Read, 1980, 1982) has resulted in production of shoot numbers equivalent to, or higher than numbers expected if the cytokinin had been incorporated into the medium in the customary fashion. While treating excised shoots with ABA or NAA was only slightly beneficial to embryo production by alfalfa protoplasts (Dijak and Brown, 1987), von Arnold and Tilberg (1987) found that pulsing buds of Picea abies with 250 ~M BA for three hours led to the highest adventitious bud yield of all treatments tested.…”

Section: Chemical and Physicalmentioning

confidence: 97%

Environmental and Hormonal Effects in Micropropagation

Read

1992

Transplant Production Systems

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ABSTRACT. Micropropagation has become important in the latter quarter of the Twentieth Century, both in a commercial sense and as an exceedingly valuable research tool for the study of plant physiology, growth and development. A myriad of papers have been published on a wide variety of subjects related to micropropagation including the critically important areas of environment and hormones. In this paper, special attention is given to the influence of environment and hormones, and their interactions as they affect the stock plant and the subsequent performance of the explant when cultured in vitro. Explant selection (genotype, organ chosen, age and physiological stage, health), stock plant management (nutrition; light intensity, quality and photoperiod; temperature; chemical and physical manipulations), gaseous atmosphere (RH, CO2, O2, ethylene) and both conventioml and novel plant growth regulating chemicals are topics presented. These topics are discussed in relation to future challenges and opportunities, such as automation and robotization of micropropagation technology.

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Somatic Embryogenesis in Herbaceous Dicots

KrishnaRaj¹,

Vasil²

1995

In Vitro Embryogenesis in Plants

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Donor tissue and culture condition effects on mesophyll protoplasts of Medicago sativa

Cited by 5 publications

References 23 publications

Plant donor tissue and isolation procedure effect on early formation of embryoids from protoplasts of Helianthus annuus L.

Plant donor tissue and isolation procedure effect on early formation of embryoids from protoplasts of Helianthus annuus L.

Environmental and Hormonal Effects in Micropropagation

Somatic Embryogenesis in Herbaceous Dicots

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